Category: 79642-50-5

On May 20, 2022, Kasu, Yasar Arfat T.; Arva, Akshaya; Johnson, Jess; Sajan, Christin; Manzano, Jasmin; Hennes, Andrew; Haynes, Jacy; Brower, Christopher S. published an article.Electric Literature of 79642-50-5 The title of the article was BAG6 prevents the aggregation of neurodegeneration-associated fragments of TDP43. And the article contained the following:

Neurodegeneration is associated with the aggregation of proteins bearing solvent-exposed hydrophobicity as a result of their misfolding and/or proteolytic cleavage. An understanding of the cellular protein quality control mechanisms which prevent protein aggregation is fundamental to understanding the etiol. of neurodegeneration. By examining the metabolism of disease-linked C-terminal fragments of the TAR DNA-binding protein 43 (TDP43), we found that the Bcl-2 associated athanogene 6 (BAG6) functions as a sensor of proteolytic fragments bearing exposed hydrophobicity and prevents their intracellular aggregation. In addition, BAG6 facilitates the ubiquitylation of TDP43 fragments by recruiting the Ub-ligase, Ring finger protein 126 (RNF126). Authenticating its role in preventing aggregation, we found that TDP43 fragments form intracellular aggregates in the absence of BAG6. Finally, we found that BAG6 could interact with and solubilize addnl. neurodegeneration-associated proteolytic fragments. Therefore, BAG6 plays a general role in preventing intracellular aggregation associated with neurodegeneration. The experimental process involved the reaction of Bis(2,5-dioxopyrrolidin-1-yl) glutarate(cas: 79642-50-5).Electric Literature of 79642-50-5

The Article related to aggregation bag6 neurodegeneration proteolytic fragment ubiquitylation tdp43 rnf126, biological sciences, cell biology, molecular biology, molecular neuroscience, neuroscience and other aspects.Electric Literature of 79642-50-5

Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics

On March 30, 2019, Hernychova, Lucie; Rosulek, Michal; Kadek, Alan; Mareska, Vaclav; Chmelik, Josef; Adamkova, Ljubina; Grobarova, Valeria; Sebesta, Ondrej; Kukacka, Zdenek; Skala, Kristian; Spiwok, Vojtech; Cerny, Jan; Novak, Petr published an article.Computed Properties of 79642-50-5 The title of the article was The C-type lectin-like receptor Nkrp1b: Structural proteomics reveals features affecting protein conformation and interactions. And the article contained the following:

Here, we characterized the Nkrp1b structure and structural features that affect its interactions. To study the Nkrp1b protein structure and the functional importance of its stalk, two Nkrp1b protein variants differing by the presence of the stalk were prepared These variants were studied using a combination of structural mass spectrometry approaches with computational modeling to derive structural models. In addition, information about biol. activity and localization in mammalian cells was acquired using scanning microscopy techniques and western blotting. Based on these methods, we obtained the structure of Nkrp1b ectodomain in its monomeric and dimeric conformations, identified the dimerization interface, and determined disulfide connections within the mol. We found that Nkrp1b occurs as a mixture of monomers and homodimers, both in vitro and in vivo. Despite the long-standing assumption that Nkrp1 proteins are homodimers connected by disulfide bonds in the stalk region, our data showed that both Nkrp1b protein variants form monomers and homodimers irresp. of the presence of the stalk. Using a unique combination of computational, biochem., and biol. methods, we revealed the structural conformation and behavior of Nkrp1b in its native state. In addition, it is a first report utilizing the intermol. chem. crosslinking of light- and heavy-labeled protein chains together with ion mobility-mass spectrometry to design the structural models of protein homodimers. The experimental process involved the reaction of Bis(2,5-dioxopyrrolidin-1-yl) glutarate(cas: 79642-50-5).Computed Properties of 79642-50-5

The Article related to proteome nkrp1b oligomerization disulfide bond protein conformation interaction, chemical cross-linking, homodimers, ion mobility, natural killer cells, nkrp1b, structural mass spectrometry and other aspects.Computed Properties of 79642-50-5

Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics

Santos, Luiz F. A.; Iglesias, Amadeu H.; Gozzo, Fabio C. published an article in 2011, the title of the article was Fragmentation features of intermolecular cross-linked peptides using N-hydroxy- succinimide esters by MALDI- and ESI-MS/MS for use in structural proteomics.Electric Literature of 79642-50-5 And the article contains the following content:

The use of mass spectrometry coupled with chem. crosslinking of proteins has become one of the most useful tools for proteins structure and interactions studies. One of the challenges in these studies is the identification of the cross-linked peptides. The interpretation of the MS/MS data generated in crosslinking experiments using N-hydroxy succinimide esters is not trivial once a new amide bond is formed allowing new fragmentation pathways, unlike linear peptides. Intermol. cross-linked peptides occur when two different peptides are connected by the cross-linker and they yield information on the spatial proximity of different domains (within a protein) or proteins (within a complex). In this article, the authors report a detailed fragmentation study of intermol. cross-linked peptides, generated from a set of synthetic peptides, using both ESI and MALDI to generate the precursor ions. The fragmentation features observed here can be helpful in the interpretation and identification of cross-linked peptides present in crosslinking experiments and be further implemented in search engine’s algorithms. Copyright © 2011 John Wiley & Sons, Ltd. The experimental process involved the reaction of Bis(2,5-dioxopyrrolidin-1-yl) glutarate(cas: 79642-50-5).Electric Literature of 79642-50-5

The Article related to maldi mass spectrometry fragmentation intermol crosslinked peptide hydroxysuccinimide ester, electrospray ionization mass spectrometry fragmentation intermol crosslinked peptide hydroxysuccinimide and other aspects.Electric Literature of 79642-50-5

Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics

Russ Algar, W.; Krull, Ulrich J. published an article in 2011, the title of the article was Interfacial chemistry and the design of solid-phase nucleic acid hybridization assays using immobilized quantum dots as donors in fluorescence resonance energy transfer.Related Products of 79642-50-5 And the article contains the following content:

The use of quantum dots (QDs) as donors in fluorescence resonance energy transfer (FRET) offer several advantages for the development of multiplexed solid-phase QD-FRET nucleic acid hybridization assays. Designs for multiplexing have been demonstrated, but important challenges remain in the optimization of these systems. In this work, we identify several strategies based on the design of interfacial chem. for improving sensitivity, obtaining lower limits of detection (LOD) and enabling the regeneration and reuse of solid-phase QD-FRET hybridization assays. FRET-sensitized emission from acceptor dyes associated with hybridization events at immobilized QD donors provides the anal. signal in these assays. The minimization of active sensing area reduces background from QD donor PL and allows the resolution of smaller amounts of acceptor emission, thus lowering the LOD. The association of multiple acceptor dyes with each hybridization event can enhance FRET efficiency, thereby improving sensitivity. Many previous studies have used interfacial protein layers to generate selectivity; however, transient destabilization of these layers is shown to prevent efficient regeneration. To this end, we report a protein-free interfacial chem. and demonstrate the specific detection of as little as 2 pmol of target, as well as an improved capacity for regeneration. The experimental process involved the reaction of Bis(2,5-dioxopyrrolidin-1-yl) glutarate(cas: 79642-50-5).Related Products of 79642-50-5

The Article related to interfacial chem nucleic acid hybridization assay quantum dot fret, dna, active area, biosensor, fluorescence resonance energy transfer, immobilization, quantum dots, regeneration, signal enhancement and other aspects.Related Products of 79642-50-5

Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics

On January 1, 2018, Zhou, Zilong; Peng, Shanshan; Sui, Minghao; Chen, Shiguang; Huang, Lishi; Xu, Hui; Jiang, Tingting published an article.Formula: C13H14N2O8 The title of the article was Multifunctional nanocomplex for surface-enhanced Raman scattering imaging and near-infrared photodynamic antimicrobial therapy of vancomycin-resistant bacteria. And the article contained the following:

Since vancomycin (Van)-resistant enterococci (VRE) strains first emerged as a serious threat to public health, extensive studies focused on optical imaging and antimicrobial therapy have been performed for monitoring and microbiol. control. In this study, we developed silicon 2,3-naphthalocyanine dihydroxide (Nc) and Van functionalized silica-encapsulated, silver-coated gold nanoparticles (Au@AgNP@SiO2@Nc-Van) as a novel theranostic system for surface-enhanced Raman scattering (SERS) detection and antimicrobial photodynamic therapy (aPDT) of VRE strains. The silver-coated gold nanoparticle, as the SERS-active core, exhibited excellent Raman enhancement efficacy. Results of in vitro bacterial SERS imaging revealed Van-enhanced specific binding affinity toward VRE. Meanwhile, Si(IV) naphthalocyanine, serving as a near-IR (NIR) photosensitizer, was axially linked to the nanoparticle surface, yielding nanostructured hybrid materials that could photoinactivate VRE. Almost 4-5 logs of bacterial reduction were obtained upon in vitro photodynamic therapy of VRE treated with a nanomolar concentration of the nanocomplex. Mouse infection assays were applied for an in vivo evaluation of VRE lethality. Upon near-IR light irradiation, this hybrid nanomaterial caused obvious infection regression and even complete eradication compared to the findings in the non-treated groups. Therefore, this novel nanosystem integrating SERS imaging and noninvasive aPDT has huge potential for applications in theranostics with regard to VRE management. The experimental process involved the reaction of Bis(2,5-dioxopyrrolidin-1-yl) glutarate(cas: 79642-50-5).Formula: C13H14N2O8

The Article related to nir radiation antimicrobial raman scattering imaging, antimicrobial photodynamic therapy, multifunctional nanoparticles, sers imaging, silver-coated gold nanoparticles, vancomycin-resistant enterococci and other aspects.Formula: C13H14N2O8

Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics

On April 30, 2020, Fiala, Jan; Kukacka, Zdenek; Novak, Petr published an article.Related Products of 79642-50-5 The title of the article was Influence of cross-linker polarity on selectivity towards lysine side chains. And the article contained the following:

The combination of chem. crosslinking and mass spectrometry is currently a progressive technol. for deriving structural information of proteins and protein complexes. In addition, chem. crosslinking is a powerful tool for stabilizing macromol. complexes for single particle cryo-electron microscopy. However, the effect of cross-linker polarity on the outcome of crosslinking reaction has never been studied. Herein, we use both polar (bis(sulfosuccinimidyl) glutarate) and non-polar (disuccinimidyl glutarate) cross-linkers and systematically investigated the impact of cross-linker hydrophobicity on resulting distance constraints, using bovine serum albumin as a model protein. Even though the amine reactive BS2G and DSG cross-linkers have the same length of spacer and are based on N-hydroxysuccinimidic group, our data showed that each of them formed preferentially different cross-links. We demonstrated that the choice of cross-linker can have a significant impact on the output data for structural characterization of biomols. Using equimolar mixtures of DSG with d6-BS2G, and BS2G with d6-DSG, we established that the polar BS2G preferentially bound to polar regions of modified mol., whereas non-polar DSG bound to hydrophobic regions. This phenomenon established that the mixture of polar and non-polar cross-linkers acted as an efficient tool for the determination of distance constraints in proteins. The experimental process involved the reaction of Bis(2,5-dioxopyrrolidin-1-yl) glutarate(cas: 79642-50-5).Related Products of 79642-50-5

The Article related to protein structure lysine cross linker polarity mass spectrometry, bis(sulfosuccinimidyl) glutarate, bovine serum albumin, chemical cross-linking, disuccinimidyl glutarate, protein structure, structural mass spectrometry and other aspects.Related Products of 79642-50-5

Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics

Park, Jong-Min; Kim, Mi Yeon; Jose, Joachim; Park, Min published an article in 2022, the title of the article was Covalently Immobilized Regenerable Immunoaffinity Layer with Orientation-Controlled Antibodies Based on Z-Domain Autodisplay.Application In Synthesis of Bis(2,5-dioxopyrrolidin-1-yl) glutarate And the article contains the following content:

A regenerable immunoaffinity layer comprising covalently immobilized orientation-controlled antibodies was developed for use in a surface plasmon resonance (SPR) biosensor. For antibody orientation control, antibody-binding Z-domain-autodisplaying Escherichia coli (E. coli) cells and their outer membrane (OM) were utilized, and a disuccinimidyl crosslinker was employed for covalent antibody binding. To fabricate the regenerable immunoaffinity layer, capture antibodies were bound to autodisplayed Z-domains, and then treated with the crosslinker for chem. fixation to the Z-domains. Various crosslinkers, namely disuccinimidyl glutarate (DSG), disuccinimidyl suberate (DSS) and poly (ethylene glycol)-ylated bis (sulfosuccinimidyl)suberate (BS(PEG)5), were evaluated, and DSS at a concentration of 500μM was confirmed to be optimal. The E. coli-cell-based regenerable HRP immunoassay was evaluated employing three sequential HRP treatment and regeneration steps. Then, the Oms of E. coli cells were isolated and layered on a microplate and regenerable OM-based HRP immunoassaying was evaluated. Five HRP immunoassays with four regeneration steps were found to be feasible. This regenerable, covalently immobilized, orientation-controlled OM-based immunoaffinity layer was applied to an SPR biosensor, which was capable of quantifying C-reactive protein (CRP). Five regeneration cycles were repeated using the demonstrated immunoaffinity layer with a signal difference of <10%. The experimental process involved the reaction of Bis(2,5-dioxopyrrolidin-1-yl) glutarate(cas: 79642-50-5).Application In Synthesis of Bis(2,5-dioxopyrrolidin-1-yl) glutarate

The Article related to covalently immobilized regenerable immunoaffinity layer orientation antibody zdomain autodisplay, spr biosensor, z-domains, autodisplay, covalent immobilization, crosslinker, immunoaffinity layer, orientation control, regeneration and other aspects.Application In Synthesis of Bis(2,5-dioxopyrrolidin-1-yl) glutarate

Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics

On October 16, 2015, Wang, Lizhen; Feng, Shaojie; An, Lian; Gu, Guofeng; Guo, Zhongwu published an article.Application In Synthesis of Bis(2,5-dioxopyrrolidin-1-yl) glutarate The title of the article was Synthetic and Immunological Studies of Mycobacterial Lipoarabinomannan Oligosaccharides and Their Protein Conjugates. And the article contained the following:

Lipoarabinomannan (LAM) is one of the major constituents of the Mycobacterium tuberculosis cell wall and an attractive mol. scaffold for antituberculosis drug and vaccine development. In this paper, a convergent strategy was developed for the synthesis of LAM oligosaccharides with an α-1,2-linked dimannopyranose cap at the nonreducing end. The strategy was highlighted by efficient coupling of sep. prepared non-reducing end and reducing end oligosaccharides. Glycosylation were mainly achieved with thioglycoside donors, which gave excellent yields and stereoselectivity even for reactions between complex oligosaccharides. The strategy was utilized to successfully synthesize tetra-, hepta-, and undecasaccharides of LAM from D-arabinose in 10, 15, and 14 longest linear steps and 7.84, 7.50, and 2.59% overall yields, resp. The resultant oligosaccharides with a free amino group at their reducing end were effectively conjugated with carrier proteins, including bovine serum albumin and keyhole limpet hemocyanin (KLH), via a bifunctional linker. Preliminary immunol. studies on the KLH conjugates revealed that they could elicit robust antibody responses in mice and that the antigen structure had some influence on their immunol. property, thus verifying the potential of the oligosaccharides for vaccine development and other immunol. studies. The experimental process involved the reaction of Bis(2,5-dioxopyrrolidin-1-yl) glutarate(cas: 79642-50-5).Application In Synthesis of Bis(2,5-dioxopyrrolidin-1-yl) glutarate

The Article related to antituberculosis vaccine oligosaccharide dimannopyranose stereoselective glycosylation were thioglycoside antibacterial, oligosaccharide preparation mycobacterial lipoarabinomannan immunol protein antibody thioglycoside glycosylation and other aspects.Application In Synthesis of Bis(2,5-dioxopyrrolidin-1-yl) glutarate

Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics

Liu, Minglong; Sovrovic, Miha; Suga, Hiroaki; Jongkees, Seino A. K. published an article in 2022, the title of the article was Phosphine addition to dehydroalanine for peptide modification.SDS of cas: 79642-50-5 And the article contains the following content:

Thiols are a functional group commonly used for selective reactions in a biochem. setting because of their high nucleophilicity. Phosphorus nucleophiles can undergo some similar reactions to thiols, but remain underexploited in this setting. In this work we show that phosphine nucleophiles react cleanly and quickly with a dehydroalanine electrophile, itself generated from cysteine, to give a stable adduct in a peptide context. NMR reveals the product to be a phosphonium ion and indicates some backbone conformational constraint, possibly arising from transient carbonyl coordination. The reaction proceeded quickly, with a pseudo-first order rate constant of 0.126 min-1 at 1 mM peptide (80% conversion in 10 min), and with no detectable side products on the peptide. A broad peptide sequence scope and water-soluble phosphines with alkyl as well as aromatic groups were all shown to react efficiently. Phosphine addition proved to be efficient on nisin as a model Dha-containing biol.-derived peptide and on an mRNA-displayed peptide, as well as on TCEP-modified agarose for peptide capture from solution This reaction thus presents a promising approach for modification of peptides for cargo attachment or altered phys. properties in peptide discovery. The experimental process involved the reaction of Bis(2,5-dioxopyrrolidin-1-yl) glutarate(cas: 79642-50-5).SDS of cas: 79642-50-5

The Article related to phosphine addition dehydroalanine peptide modification thiol cysteine, peptide addition phosphine reaction kinetics product distribution decomposition stability, solid phase peptide synthesis phosphine addition, nisin rna peptide phosphine addition and other aspects.SDS of cas: 79642-50-5

Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics

On November 20, 2020, Wang, Junchang; Zhang, Yiyue; Zhu, Yirong; Liu, Junru; Chen, Yan; Cao, Xin; Yang, You published an article.Application of 79642-50-5 The title of the article was Total Synthesis and Immunological Evaluation of the Tri-D-glycero-D-manno-heptose Antigen of the Lipopolysaccharide as a Vaccine Candidate against Helicobacter pylori. And the article contained the following:

Helicobacter pylori, the most common cause of chronic gastritis, peptic ulcers and gastric cancers, infects around half of the world’s population. Although the drawbacks of the antibiotic-based combination therapy are emerging, no effective vaccine is available to prevent H. pylori infections. Here, we describe the total synthesis of the unique α-(1→3)-linked triD-glycero-D-manno-heptose antigen from the lipopolysaccharide of H. pylori sero-groups O3, O6 and strains MO19, D2, D4 and D5 based on de novo synthesis of the differentially protected D-glycero-D-manno-heptosyl building blocks. Immunization of mice with the semi-synthetic glycoconjugate elicited a very robust T-cell dependent antigen-specific immune response, resulting in very high titers of IgG1 and IgG2b protective antibody iso-types. The post-immune sera recognized H. pylori NCTC 11637 and bound strongly to the surface of the intact bacteria. The experimental process involved the reaction of Bis(2,5-dioxopyrrolidin-1-yl) glutarate(cas: 79642-50-5).Application of 79642-50-5

The Article related to human serum albumin crm197 diphteria toxin glycoconjugate, antibiotic antigen antitumor combination chemotherapy immunization glycoconjugate ig, lipopolysaccharide glyceromannoheptose antigen immunol synthesis vaccine helicobacter pylori antibacterial and other aspects.Application of 79642-50-5

Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics