Category: 79642-50-5

Yoneda, Kozo; Hu, Yaping; Watanabe, Rei; Kita, Masaki; Kigoshi, Hideo published an article in 2016, the title of the article was Binding position analysis of target proteins with the use of amidopyrene probes as LA-LDI enhancing tags.Safety of Bis(2,5-dioxopyrrolidin-1-yl) glutarate And the article contains the following content:

Amidopyrene-conjugated compounds can be detected by label-assisted laser desorption/ionization mass spectrometry (LA-LDI MS) without matrixes. When actin, a cytoskeletal protein, was labeled with an excess amount of amidopyrene N-hydroxysuccinate (apy-OSu), eight apy-labeled actin peptides were predominantly detected by LA-LDI MS. Then actin was labeled with an amidopyrene NHS ester of the antitumor marine macrolide aplyronine A (ApA-apy-OSu) to form a 1 : 1 conjugate. The sequence of an apy-labeled peptide was established as A108PLNPKANR116 by MS/MS anal., in which the NHS ester moiety specifically reacted with the ε-amino group of K113. While the fragmentation at the linker part reduces the detection sensitivity of apy-labeled peptides on LA-LDI MS, our chem. probe method is useful for analyzing the binding modes of various ligands and target biomacromols. that include multiple and weak interactions. The experimental process involved the reaction of Bis(2,5-dioxopyrrolidin-1-yl) glutarate(cas: 79642-50-5).Safety of Bis(2,5-dioxopyrrolidin-1-yl) glutarate

The Article related to binding position analysis protein amidopyrene probe affinity labeling ms, Biochemical Methods: Other (Not Covered At Other Subsections) and other aspects.Safety of Bis(2,5-dioxopyrrolidin-1-yl) glutarate

Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics

On February 1, 2011, Cyrus, Kedra; Wehenkel, Marie; Choi, Eun-Young; Han, Hyeong-Jun; Lee, Hyosung; Swanson, Hollie; Kim, Kyung-Bo published an article.Application In Synthesis of Bis(2,5-dioxopyrrolidin-1-yl) glutarate The title of the article was Impact of linker length on the activity of PROTACs. And the article contained the following:

Conventional genetic approaches have provided a powerful tool in the study of proteins. However, these techniques often preclude selective manipulation of temporal and spatial protein functions, which is crucial for the investigation of dynamic cellular processes. To overcome these limitations, a small mol.-based novel technol. termed “PROteolysis TArgeting ChimeraS (PROTACs)” has been developed, targeting proteins for degradation at the post-translational level. Despite the promising potential of PROTACs to serve as mol. probes of complex signaling pathways, their design has not been generalized for broad application. Here, we present the first generalized approach for PROTAC design by fine-tuning the distance between the two participating partner proteins, the E3 ubiquitin ligase and the target protein. As such, we took a chem. approach to create estrogen receptor (ER)-α targeting PROTACs with varying linker lengths and the loss of the ER in cultured cells was monitored via western blot and fluorometric analyses. We found a significant effect of chain length on PROTAC efficacy, and, in this case, the optimum distance between the E3 recognition motif and the ligand was a 16 atom chain length. The information gathered from this experiment may offer a generalizable PROTAC design strategy to further the expansion of the PROTAC toolbox, opening new possibilities for the broad application of the PROTAC strategy in the study of multiple signaling pathways. The experimental process involved the reaction of Bis(2,5-dioxopyrrolidin-1-yl) glutarate(cas: 79642-50-5).Application In Synthesis of Bis(2,5-dioxopyrrolidin-1-yl) glutarate

The Article related to protein linker length breast cancer protac western blot fluorometry, Biochemical Methods: Other (Not Covered At Other Subsections) and other aspects.Application In Synthesis of Bis(2,5-dioxopyrrolidin-1-yl) glutarate

Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics

van Dongen, Stijn F. M.; Janvore, Julie; van Berkel, Sander S.; Marie, Emmanuelle; Piel, Matthieu; Tribet, Christophe published an article in 2012, the title of the article was Reactive protein-repellent surfaces for the straightforward attachment of small molecules up to whole cells.Quality Control of Bis(2,5-dioxopyrrolidin-1-yl) glutarate And the article contains the following content:

Here we present a readily accessible strategy for creating protein-resistant surfaces that contain azides, based on the spontaneous adsorption of a comb-like copolymer. Using a strain-promoted azide alkyne cycloaddition, we illustrate the single-step conjugation of various representatives of important applications, being the labeling with small organic mols., capture of nanoparticles, attachment of native enzymes at a controlled d., and finally control of cell adhesion. We thus demonstrate that advanced biomaterials can be created in two straightforward steps without requiring addnl. reagents, which should make this strategy a valuable tool for researchers in diverse fields. The experimental process involved the reaction of Bis(2,5-dioxopyrrolidin-1-yl) glutarate(cas: 79642-50-5).Quality Control of Bis(2,5-dioxopyrrolidin-1-yl) glutarate

The Article related to protein repellent surface mol cell, Biochemical Methods: Other (Not Covered At Other Subsections) and other aspects.Quality Control of Bis(2,5-dioxopyrrolidin-1-yl) glutarate

Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics

Cao, Cheng; Zhao, Peng; Li, Ze; Chen, Zitian; Huang, Yanyi; Bai, Yu; Li, Xiaoyu published an article in 2014, the title of the article was A DNA-templated synthesis of encoded small molecules by DNA self-assembly.Recommanded Product: 79642-50-5 And the article contains the following content:

We report a novel method for the synthesis of DNA-encoded libraries without the need for discrete DNA template. Reactant DNAs self-assemble to enable chem. reactions and photo-cleavage transfers the product to the DNA terminus, making it suitable for the subsequent affinity-based selection and hit deconvolution. The experimental process involved the reaction of Bis(2,5-dioxopyrrolidin-1-yl) glutarate(cas: 79642-50-5).Recommanded Product: 79642-50-5

The Article related to dna templated synthesis acylthiazolidine dihydropyran self assembly library, Biochemical Methods: Other (Not Covered At Other Subsections) and other aspects.Recommanded Product: 79642-50-5

Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics

On January 17, 2012, Rozbesky, Daniel; Man, Petr; Kavan, Daniel; Chmelik, Josef; Cerny, Jiri; Bezouska, Karel; Novak, Petr published an article.Recommanded Product: 79642-50-5 The title of the article was Chemical Cross-Linking and H/D Exchange for Fast Refinement of Protein Crystal Structure. And the article contained the following:

A combination of chem. crosslinking and hydrogen-deuterium exchange coupled to high resolution mass spectrometry was used to describe structural differences of NKR-P1A receptor. The loop region extended from the compact core in the crystal structure is closely attached to the protein core in solution The authors’ approach has potential to refine protein structures in solution within a few days and has very low sample consumption. The experimental process involved the reaction of Bis(2,5-dioxopyrrolidin-1-yl) glutarate(cas: 79642-50-5).Recommanded Product: 79642-50-5

The Article related to crosslinking hydrogen deuterium exchange protein structure refinement mass spectrometry, Biochemical Methods: Other (Not Covered At Other Subsections) and other aspects.Recommanded Product: 79642-50-5

Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics

On October 12, 2020, Bacon, Kaitlyn; Blain, Abigail; Burroughs, Matthew; McArthrur, Nikki; Rao, Balaji M.; Menegatti, Stefano published an article.Name: Bis(2,5-dioxopyrrolidin-1-yl) glutarate The title of the article was Isolation of Chemically Cyclized Peptide Binders Using Yeast Surface Display. And the article contained the following:

Cyclic peptides with engineered protein-binding activity have gained increasing attention for use in therapeutic and biotechnol. applications. The authors describe the efficient isolation and characterization of cyclic peptide binders from genetically encoded combinatorial libraries using yeast surface display. Here, peptide cyclization is achieved by disuccinimidyl glutarate-mediated crosslinking of amine groups within a linear peptide sequence that is expressed as a yeast cell surface fusion. Using this approach, the authors first screened a library of cyclic heptapeptides using magnetic selection, followed by fluorescence activated cell sorting (FACS) to isolate binders for a model target (lysozyme) with low micromolar binding affinity (KD ~1.2-3.7μM). The isolated peptides bind lysozyme selectively and only when cyclized. Importantly, yeast surface displayed cyclic peptides can be used to efficiently obtain quant. estimates of binding affinity, circumventing the need for chem. synthesis of the selected peptides. Subsequently, to demonstrate broader applicability of the authors’ approach, the authors isolated cyclic heptapeptides that bind human interleukin-17 (IL-17) using yeast-displayed IL-17 as a target for magnetic selection, followed by FACS using recombinant IL-17. Mol. docking simulations and follow-up exptl. analyses identified a candidate cyclic peptide that likely binds IL-17 in its receptor binding region with moderate apparent affinity (KD ~300 nM). Taken together, the authors’ results show that yeast surface display can be used to efficiently isolate and characterize cyclic peptides generated by chem. modification from combinatorial libraries. The experimental process involved the reaction of Bis(2,5-dioxopyrrolidin-1-yl) glutarate(cas: 79642-50-5).Name: Bis(2,5-dioxopyrrolidin-1-yl) glutarate

The Article related to cyclized peptide binder yeast surface display, affinity ligands, cyclic peptides, interleukin-17 (il-17), library screening, yeast-display libraries, Biochemical Methods: Other (Not Covered At Other Subsections) and other aspects.Name: Bis(2,5-dioxopyrrolidin-1-yl) glutarate

Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics

Corbille, Anne-Gaelle; Neunlist, Michel; Derkinderen, Pascal published an article in 2016, the title of the article was Cross-linking for the analysis of α-synuclein in the enteric nervous system.Quality Control of Bis(2,5-dioxopyrrolidin-1-yl) glutarate And the article contains the following content:

Since the observation that aggregated α-synuclein, the pathol. hallmark of Parkinson’s disease (PD), is found in the gut in almost all patients, it has been suggested that the enteric nervous system (ENS) could be a starting point for α-synuclein pathol. α-synuclein has long been thought to occur as a monomer in living cells, but recent studies reported that it instead exists as a tetramer in non-neuronal cells and in neurons. Given the possible key role of the ENS in PD pathophysiol., we undertook the current research to characterize the native state of α-synuclein in rat primary culture of ENS and in adult human healthy ENS. Using amine-reactive crosslinking, we showed that, by contrast to cell lines and brain neurons, α-synuclein exists primarily as a monomer in intact enteric neurons, suggesting that the native state of α-synuclein is different between the ENS and the brain. Our results provide new insights into the widely discussed concepts of α-synuclein aggregation and misfolding in PD and raise issue about the possible transmission of α-synuclein from the ENS to the brain. Aggregated α-synuclein is found in the gut in almost all Parkinson’s disease patients. We used amine-reactive crosslinking to study the native state of α-synuclein in the enteric nervous system (ENS). We showed that, by contrast to erythroid cells and brain neurons, α-synuclein exists primarily as a monomer in intact enteric neurons. Our results provide new insights into the possible role of the ENS in the pathophysiol. of Parkinson’s disease. The experimental process involved the reaction of Bis(2,5-dioxopyrrolidin-1-yl) glutarate(cas: 79642-50-5).Quality Control of Bis(2,5-dioxopyrrolidin-1-yl) glutarate

The Article related to synuclein analysis crosslinking enteric nervous system brain human parkinson, parkinson’s disease, cross-linking, enteric nervous system, α-synuclein, Biochemical Methods: Other (Not Covered At Other Subsections) and other aspects.Quality Control of Bis(2,5-dioxopyrrolidin-1-yl) glutarate

Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics

On November 1, 2015, Kukacka, Zdenek; Rosulek, Michal; Strohalm, Martin; Kavan, Daniel; Novak, Petr published an article.Application of 79642-50-5 The title of the article was Mapping protein structural changes by quantitative cross-linking. And the article contained the following:

Chem. crosslinking is a promising technol. for protein tertiary structure determination Though the data has low spatial resolution, it is possible to obtain it at physiol. conditions on proteins that are not amenable to standard high resolution techniques such as X-ray, NMR anal. and cryo-EM. Here we demonstrate the utilization of isotopically labeled chem. crosslinking to visualize protein conformation rearrangements. Since calmodulin exists in two distinct conformations (calcium-free and calcium-containing forms), we selected this protein for testing the potential and the limits of a new technique. After crosslinking of both calmodulin forms, the calcium-free and calcium-containing forms were mixed together and digested under different conditions and the products of proteolysis were monitored using high resolution mass spectrometry. Finally, the ratios of heavy/light cross-links were calculated by mMass open source platform. The experimental process involved the reaction of Bis(2,5-dioxopyrrolidin-1-yl) glutarate(cas: 79642-50-5).Application of 79642-50-5

The Article related to protein mapping structural change quant cross linking, chemical cross-linking, mass spectrometry, protein structure design, proteolysis, quantification, Biochemical Methods: Other (Not Covered At Other Subsections) and other aspects.Application of 79642-50-5

Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics

On December 31, 2014, McEnaney, Patrick J.; Fitzgerald, Kelly J.; Zhang, Andrew X.; Douglass, Eugene F. Jr.; Shan, Weifang; Balog, Aaron; Kolesnikova, Mariya D.; Spiegel, David A. published an article.Recommanded Product: Bis(2,5-dioxopyrrolidin-1-yl) glutarate The title of the article was Chemically Synthesized Molecules with the Targeting and Effector Functions of Antibodies. And the article contained the following:

This article reports the design, synthesis, and evaluation of a novel class of mols. of intermediate size (approx. 7000 Da), which possess both the targeting and effector functions of antibodies. These compounds-called synthetic antibody mimics targeting prostate cancer (SyAM-Ps)-bind simultaneously to prostate-specific membrane antigen and Fc gamma receptor I, thus eliciting highly selective cancer cell phagocytosis. SyAMs have the potential to combine the advantages of both small-mol. and biol. therapies, and may address many drawbacks associated with available treatments for cancer and other diseases. The experimental process involved the reaction of Bis(2,5-dioxopyrrolidin-1-yl) glutarate(cas: 79642-50-5).Recommanded Product: Bis(2,5-dioxopyrrolidin-1-yl) glutarate

The Article related to antitumor neoplasm prostate specific membrane antigen, Pharmacology: Effects Of Neoplasm Inhibitors and Cytotoxic Agents and other aspects.Recommanded Product: Bis(2,5-dioxopyrrolidin-1-yl) glutarate

Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics

On March 20, 2019, Kishimoto, Satoshi; Nakashimada, Yuichi; Yokota, Riri; Hatanaka, Takaaki; Adachi, Motoyasu; Ito, Yuji published an article.Safety of Bis(2,5-dioxopyrrolidin-1-yl) glutarate The title of the article was Site-Specific Chemical Conjugation of Antibodies by Using Affinity Peptide for the Development of Therapeutic Antibody Format. And the article contained the following:

Artificially modified IgG mols. are increasingly utilized in industrial and clin. applications. In the present study, the method of chem. conjugation by affinity peptide (CCAP) for site-specific chem. modification has been developed by using a peptide that bound with high affinity to human IgG-Fc. This method enabled a rapid modification of a specific residue (Lys248 on Fc) in a one-step reaction under mild condition to form a stable amide bond between the peptide and Fc. The monovalent peptide-IgG conjugate not only maintained complete antigen binding but also bound to Fc receptors (FcRn, FcγRI, and FcγRIIIa), indicating that it is a suitable conjugate form that can be further developed into highly functional antibody therapeutics. CCAP was applied for the preparation of an antibody-drug conjugate and a bispecific antibody to demonstrate the usefulness of this method. The experimental process involved the reaction of Bis(2,5-dioxopyrrolidin-1-yl) glutarate(cas: 79642-50-5).Safety of Bis(2,5-dioxopyrrolidin-1-yl) glutarate

The Article related to igg fc heavy chain peptide conjugation drug conjugate bispecific, Pharmacology: Effects Of Neoplasm Inhibitors and Cytotoxic Agents and other aspects.Safety of Bis(2,5-dioxopyrrolidin-1-yl) glutarate

Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics