Category: 79642-50-5

Zhou, Zhifang; Ding, Wenzhang; Li, Chen; Wu, Zhimeng published an article in 2017, the title of the article was Synthesis and immunological study of a wall teichoic acid-based vaccine against E. faecium U0317.Safety of Bis(2,5-dioxopyrrolidin-1-yl) glutarate And the article contains the following content:

A repeat unit of cell wall teichoic acids (WTA) isolated from Enterococcus faecium U0317 was chem. synthesized efficiently by a stepwise strategy. It was derivatized with a 5-aminopentanyl linker to facilitate conjugation with carrier proteins KLH and HSA. Immunol. studies of the KLH conjugate demonstrated that it could provoke robust immune responses and high titers of IgG antibodies, which could successfully recognize the synthesized WTA repeat unit . This result suggested that synthetic glycoconjugate could be a promising vaccine candidate against E. faecium for further studies. The experimental process involved the reaction of Bis(2,5-dioxopyrrolidin-1-yl) glutarate(cas: 79642-50-5).Safety of Bis(2,5-dioxopyrrolidin-1-yl) glutarate

The Article related to synthesis wall teichoic acid vaccine, Immunochemistry: Adjuvants, Antigens, Haptens, and Vaccines and other aspects.Safety of Bis(2,5-dioxopyrrolidin-1-yl) glutarate

Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics

On March 18, 2015, Liao, Guochao; Zhou, Zhifang; Burgula, Srinivas; Liao, Jun; Yuan, Cheng; Wu, Qiuye; Guo, Zhongwu published an article.Recommanded Product: Bis(2,5-dioxopyrrolidin-1-yl) glutarate The title of the article was Synthesis and Immunological Studies of Linear Oligosaccharides of β-Glucan As Antigens for Antifungal Vaccine Development. And the article contained the following:

Antifungal vaccines have recently engendered considerable excitement for counteracting the resurgence of fungal infections. In this context, β-glucan, which is abundantly expressed on all fungal cell surfaces, functionally necessary for fungi, and immunol. active, is an attractive target antigen. Aiming at the development of effective antifungal vaccines based on β-glucan, a series of its oligosaccharide derivatives was designed, synthesized, and coupled with a carrier protein, keyhole limpet hemocyanin (KLH), to form new semisynthetic glycoconjugate vaccines. In this article, a convergent and effective synthetic strategy using preactivation-based iterative glycosylation was developed for the designed oligosaccharides. The strategy can be widely useful for rapid construction of large oligo-β-glucans with shorter oligosaccharides as building blocks. The KLH conjugates of the synthesized β-glucan hexa-, octa-, deca-, and dodecasaccharides were demonstrated to elicit high titers of antigen-specific total and IgG antibodies in mice, suggesting the induction of functional T cell-mediated immunity. Moreover, it was revealed that octa-, deca-, and dodeca-β-glucans were much more immunogenic than the hexamer and that the octamer was the best among these. The results suggested that the optimal oligosaccharide sequence of β-glucan required for exceptional immunogenicity was a hepta- or octamer and that longer glucans are not necessarily better antigens, a finding that may be of general importance. Most importantly, the octa-β-glucan-KLH conjugate provoked protective immunity against Candida albicans infection in a systemic challenge model in mice, suggesting the great potential of this glycoconjugate as a clin. useful immunoprophylactic antifungal vaccine. The experimental process involved the reaction of Bis(2,5-dioxopyrrolidin-1-yl) glutarate(cas: 79642-50-5).Recommanded Product: Bis(2,5-dioxopyrrolidin-1-yl) glutarate

The Article related to oligosaccharide beta glucan antifungal vaccine, Immunochemistry: Adjuvants, Antigens, Haptens, and Vaccines and other aspects.Recommanded Product: Bis(2,5-dioxopyrrolidin-1-yl) glutarate

Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics

On November 1, 2019, Li, Qingjiang; Jiang, Wenjie; Guo, Jiatong; Jaiswal, Mohit; Guo, Zhongwu published an article.Formula: C13H14N2O8 The title of the article was Synthesis of Lewis Y Analogues and Their Protein Conjugates for Structure-Immunogenicity Relationship Studies of Lewis Y Antigen. And the article contained the following:

Analogs of cancer-associated Lewis Y (Ley) antigen with varying structures at the reducing end were synthesized by a highly efficient strategy involving one-pot preactivation-based iterative glycosylation to obtain the key tetra-/pentasaccharide intermediates, which was followed by stereoselective fucosylation. After global deprotection, these oligosaccharides were coupled with carrier protein keyhole limpet hemocyanin. The resultant glycan-protein conjugates were subjected to immunol. studies in mice. It was disclosed that the conjugate of the pentasaccharide analog of Lewis Y antigen was more immunogenic than that of the hexasaccharide analog, but the antisera of both conjugates could indiscriminately recognize each carbohydrate hapten. These results suggested that the short Lewis Y analog may be utilized to develop functional conjugate cancer vaccines. More importantly, the results also proved that the reducing-end glucose residue in the hexasaccharide analog of Lewis Y was probably not involved in its interaction with the immune system, whose discovery can have a broad impact on the design of new cancer vaccines. The experimental process involved the reaction of Bis(2,5-dioxopyrrolidin-1-yl) glutarate(cas: 79642-50-5).Formula: C13H14N2O8

The Article related to lewis y analog protein conjugate structure immunity relationship, Immunochemistry: Adjuvants, Antigens, Haptens, and Vaccines and other aspects.Formula: C13H14N2O8

Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics

Zhao, Yisheng; Wang, Subo; Wang, Guirong; Li, Hui; Guo, Zhongwu; Gu, Guofeng published an article in 2019, the title of the article was Synthesis and immunological studies of group A Streptococcus cell-wall oligosaccharide-streptococcal C5a peptidase conjugates as bivalent vaccines.Electric Literature of 79642-50-5 And the article contains the following content:

Group A Streptococcus (GAS) cell-wall polysaccharides and streptococcal C5a peptidase (ScpA) are identified as potential target antigens for the development of anti-GAS vaccines. Structurally well-defined mono-, di-, and trimers of the trisaccharide repeating unit of the major and conserved cell-wall polysaccharide of various GAS serotypes were synthesized by a convergent and efficient strategy. These synthetic oligosaccharides, which had a free amino group at the reducing end, were conjugated with a novel ScpA mutant ScpA193 protein through the bifunctional glutaryl linker. The resultant neoglycoproteins were evaluated as conjugate vaccines and compared with other glycoconjugates using the common carrier proteins diphtheria toxin mutant CRM197 and tetanus toxoid (TT). Immunol. studies using mice revealed that the ScpA193 conjugates could stimulate not only robust GAS oligosaccharide-specific antibody responses that were comparable to the immunol. activities of CRM197 and TT conjugates but also robust ScpA193-specific antibodies, making the ScpA193-oligosaccharide conjugates promising bivalent anti-GAS vaccine candidates. The experimental process involved the reaction of Bis(2,5-dioxopyrrolidin-1-yl) glutarate(cas: 79642-50-5).Electric Literature of 79642-50-5

The Article related to tetanus crm197 scpa193 streptococcus polysaccharide conjugate vaccine, Immunochemistry: Adjuvants, Antigens, Haptens, and Vaccines and other aspects.Electric Literature of 79642-50-5

Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics

Liao, Guochao; Zhou, Zhifang; Guo, Zhongwu published an article in 2015, the title of the article was Synthesis and immunological study of α-2,9-oligosialic acid conjugates as anti-group C meningitis vaccines.Safety of Bis(2,5-dioxopyrrolidin-1-yl) glutarate And the article contains the following content:

α-2,9-Di-, tri-, tetra-, and pentasialic acids were prepared and conjugated with a carrier protein. The resultant glycoconjugates elicited robust T cell-mediated immunity in mice. α-2,9-Trisialic acid was identified as a promising antigen for developing glycoconjugate vaccines against group C Neisseria meningitidis. The experimental process involved the reaction of Bis(2,5-dioxopyrrolidin-1-yl) glutarate(cas: 79642-50-5).Safety of Bis(2,5-dioxopyrrolidin-1-yl) glutarate

The Article related to synthesis immunol oligosialate conjugate neisseria antibacterial vaccine meningitis, Immunochemistry: Adjuvants, Antigens, Haptens, and Vaccines and other aspects.Safety of Bis(2,5-dioxopyrrolidin-1-yl) glutarate

Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics

On February 12, 2016, Liao, Guochao; Zhou, Zhifang; Liao, Jun; Zu, Luning; Wu, Qiuye; Guo, Zhongwu published an article.Recommanded Product: Bis(2,5-dioxopyrrolidin-1-yl) glutarate The title of the article was 6-O-branched oligo-β-glucan-based antifungal glycoconjugate vaccines. And the article contained the following:

With the rapid growth in fungal infections and drug-resistant fungal strains, antifungal vaccines have become an especially attractive strategy to tackle this important health problem. β-Glucans, a class of extracellular carbohydrate antigens abundantly and consistently expressed on fungal cell surfaces, are intriguing epitopes for antifungal vaccine development. β-Glucans have a conserved β-1,3-glucan backbone with sporadic β-1,3- or β-1,6-linked short glucans as branches at the 6-O-positions, and the branches may play a critical role in their immunol. functions. To study the immunol. properties of branched β-glucans and develop β-glucan-based antifungal vaccines, three branched β-glucan oligosaccharides with 6-O-linked β-1,6-tetraglucose, β-1,3-diglucose, and β-1,3-tetraglucose branches on a β-1,3-nonaglucan backbone, which mimic the structural epitopes of natural β-glucans, were synthesized and coupled with keyhole limpet hemocyanin (KLH) to form novel synthetic conjugate vaccines. These glycoconjugates were proved to elicit strong IgG antibody responses in mice. It was also discovered that the number, size, and structure of branches linked to the β-glucan backbone had a significant impact on the immunol. property. Moreover, antibodies induced by the synthetic oligosaccharide-KLH conjugates were able to recognize and bind to natural β-glucans and fungal cells. Most importantly, these conjugates elicited effective protection against systemic Candida albicans infection in mice. Thus, branched oligo-β-glucans were identified as functional epitopes for antifungal vaccine design and the corresponding protein conjugates as promising antifungal vaccine candidates. The experimental process involved the reaction of Bis(2,5-dioxopyrrolidin-1-yl) glutarate(cas: 79642-50-5).Recommanded Product: Bis(2,5-dioxopyrrolidin-1-yl) glutarate

The Article related to branched beta glucan antifungal glycoconjugate vaccine, carbohydrate, fungus, glycoconjugate, vaccine, β-glucan, Immunochemistry: Adjuvants, Antigens, Haptens, and Vaccines and other aspects.Recommanded Product: Bis(2,5-dioxopyrrolidin-1-yl) glutarate

Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics

On March 22, 2017, Mandal, Satadru Sekhar; Duncombe, Lucy; Ganesh, N. Vijaya; Sarkar, Susmita; Howells, Laurence; Hogarth, Philip J.; Bundle, David R.; McGiven, John published an article.SDS of cas: 79642-50-5 The title of the article was Novel solutions for vaccines and diagnostics to combat brucellosis. And the article contained the following:

Brucellosis is diagnosed by detection of antibodies in the blood of animals and humans that are specific for two carbohydrate antigens, termed A and M, which are present concurrently in a single cell wall O-polysaccharide. Animal brucellosis vaccines contain these antigenic determinants, and consequently infected and vaccinated animals cannot be differentiated as both groups produce A and M specific antibodies. We hypothesized that chem. synthesis of a pure A vaccine would offer unique identification of infected animals by a synthetic M diagnostic antigen that would not react with antibodies generated by this vaccine. Two forms of the A antigen, a hexasaccharide and a heptasaccharide conjugated to tetanus toxoid via reducing and nonreducing terminal sugars, were synthesized and used as lead vaccine candidates. Mouse antibody profiles to these immunogens showed that to avoid reaction with diagnostic M antigen it was essential to maximize the induction of anti-A antibodies that bind internal oligosaccharide sequences and minimize production of antibodies directed toward the terminal nonreducing monosaccharide. This objective was achieved by conjugation of Brucella O-polysaccharide to tetanus toxoid via its periodate oxidized terminal nonreducing monosaccharide, thereby destroying terminal epitopes and focusing the antibody response on internal A epitopes. This establishes the method to resolve the decades-long challenge of how to create effective brucellosis vaccines without compromising diagnosis of infected animals. The experimental process involved the reaction of Bis(2,5-dioxopyrrolidin-1-yl) glutarate(cas: 79642-50-5).SDS of cas: 79642-50-5

The Article related to vaccine brucella a antigen polysaccharide tetanus toxoid brucellosis, diagnosis brucellosis brucella synthetic m antigen polysaccharide, Immunochemistry: Adjuvants, Antigens, Haptens, and Vaccines and other aspects.SDS of cas: 79642-50-5

Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics

Zhou, Zhifang; Liao, Guochao; Mandal, Satadru S.; Suryawanshi, Sharad; Guo, Zhongwu published an article in 2015, the title of the article was A fully synthetic self-adjuvanting globo H-Based vaccine elicited strong T cell-mediated antitumor immunity.Product Details of 79642-50-5 And the article contains the following content:

Therapeutic cancer vaccines based on the abnormal glycans expressed on cancer cells, such as the globo H antigen, have witnessed great progress in recent years. For example, the keyhole limpet hemocyanin (KLH) conjugate of globo H has been on clin. trials as a cancer vaccine. However, such vaccines have intrinsic problems, such as inconsistence in eliciting T cell-mediated immunity in cancer patients and difficult quality control. To address the issue, a structurally defined fully synthetic glycoconjugate vaccine composed of globo H and monophosphoryl lipid A (MPLA) was developed. The new vaccine was shown to elicit robust IgG1 antibody responses and T cell-dependent immunity, which is desired for anticancer vaccines, and induce significantly faster and stronger immune responses than the globo H-KLH conjugate. Moreover, it was self-adjuvanting, namely, inducing immune responses without the use of an external adjuvant, thus MPLA was not only a vaccine carrier but also a build-in adjuvant. It was also found that antibodies induced by the new vaccine could selectively bind to and mediate strong complement-dependent cytotoxicity to globo H-expressing MCF-7 cancer cell. All of the results have demonstrated that the globo H-MPLA conjugate is a better cancer vaccine than the globo H-KLH conjugate under exptl. conditions and is worth further investigation and development. The experimental process involved the reaction of Bis(2,5-dioxopyrrolidin-1-yl) glutarate(cas: 79642-50-5).Product Details of 79642-50-5

The Article related to t cell antitumor immunity self adjuvanting globo h vaccine, mcf-7 cancer cell, cancer vaccine, globo h, glycoconjugate, monophosphoryl lipid a, Immunochemistry: Adjuvants, Antigens, Haptens, and Vaccines and other aspects.Product Details of 79642-50-5

Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics

On July 1, 2019, Liao, Jun; Pan, Bo; Liao, Guochao; Zhao, Qingjie; Gao, Yun; Chai, Xiaoyun; Zhuo, Xiaobin; Wu, Qiuye; Jiao, Binghua; Pan, Weihua; Guo, Zhongwu published an article.SDS of cas: 79642-50-5 The title of the article was Synthesis and immunological studies of β-1,2-mannan-peptide conjugates as antifungal vaccines. And the article contained the following:

Fungal cell surface carbohydrates and proteins are useful antigens for the development of antifungal vaccines. In this study, glycopeptides consisting of the β-1,2-mannan and N-terminal peptide epitopes of Candida albicans (C. albicans) cell wall phosphomannan complex and Als1p (rAls1p-N) protein, resp., were synthesized and covalently conjugated with keyhole limpet hemocyanin (KLH) and human serum albumin (HSA) through homobifunctional disuccinimidyl glutarate. The resultant KLH-conjugates were immunol. evaluated using Balb/c mice to reveal that they induced high levels of IgG antibodies. Furthermore, these conjugates showed self-adjuvanting property, as they could promote robust antibody responses without the presence of an external adjuvant. More significantly, the obtained antisera could effectively recognize both the carbohydrate and the Als1 peptide epitopes and immunofluorescence and flow cytometry assays also demonstrated that the elicited antibodies could react with the cell surface of a number of fungi, including C. albicans, C. tropicalis, C. lusitaniae and C. glabrata. These results suggested the great potential of these conjugates as antifungal vaccines. The experimental process involved the reaction of Bis(2,5-dioxopyrrolidin-1-yl) glutarate(cas: 79642-50-5).SDS of cas: 79642-50-5

The Article related to fungus candida oligosaccharide als1 peptide glycopeptide glycoconjugate antifungal vaccine, candida albicans, fungus, glycoconjugate, glycopeptide, vaccine, β-1,2-mannan, Immunochemistry: Adjuvants, Antigens, Haptens, and Vaccines and other aspects.SDS of cas: 79642-50-5

Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics

On June 30, 2018, Xiao, Kunhong; Zhao, Yang; Choi, Minjung; Liu, Hongda; Blanc, Adi; Qian, Jiang; Cahill, Thomas J. III; Li, Xue; Xiao, Yunfang; Clark, Lisa J.; Li, Sheng published an article.Application of 79642-50-5 The title of the article was Revealing the architecture of protein complexes by an orthogonal approach combining HDXMS, CXMS, and disulfide trapping. And the article contained the following:

Many cellular functions necessitate structural assemblies of two or more associated proteins. The structural characterization of protein complexes using standard methods, such as X-ray crystallog., is challenging. Herein, we describe an orthogonal approach using hydrogen-deuterium-exchange mass spectrometry (HDXMS), crosslinking mass spectrometry (CXMS), and disulfide trapping to map interactions within protein complexes. HDXMS measures changes in solvent accessibility and hydrogen bonding upon complex formation; a decrease in HDX rate could account for newly formed intermol. or intramol. interactions. To distinguish between inter- and intramol. interactions, we use a CXMS method to determine the position of direct interface regions by trapping intermol. residues in close proximity to various crosslinkers (e.g., disuccinimidyl adipate (DSA)) of different lengths and reactive groups. Both MS-based experiments are performed on high-resolution mass spectrometers (e.g., an Orbitrap Elite hybrid mass spectrometer). The physiol. relevance of the interactions identified through HDXMS and CXMS is investigated by transiently co-expressing cysteine mutant pairs, one mutant on each protein at the discovered interfaces, in an appropriate cell line, such as HEK293. Disulfide-trapped protein complexes are formed within cells spontaneously or are facilitated by addition of oxidation reagents such as H2O2 or diamide. Western blotting anal., in the presence and absence of reducing reagents, is used to determine whether the disulfide bonds are formed in the proposed complex interface in physiol. relevant milieus. The procedure described here requires 1-2 mo. We demonstrate this approach using the β2-adrenergic receptor-β-arrestin1 complex as the model system. The experimental process involved the reaction of Bis(2,5-dioxopyrrolidin-1-yl) glutarate(cas: 79642-50-5).Application of 79642-50-5

The Article related to hdxms cxms disulfide trapping combining orthogonal approach protein complex, Biochemical Methods: Other (Not Covered At Other Subsections) and other aspects.Application of 79642-50-5

Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics