Category: 79642-50-5

On March 7, 2011, McKee, Mireya L.; Evans, Amanda C.; Gerrard, Simon R.; O’Reilly, Rachel K.; Turberfield, Andrew J.; Stulz, Eugen published an article.Name: Bis(2,5-dioxopyrrolidin-1-yl) glutarate The title of the article was Peptidomimetic bond formation by DNA-templated acyl transfer. And the article contained the following:

The efficiencies of DNA-templated acyl transfer reactions between a thioester modified oligonucleotide and a series of amine and thiol based nucleophiles are directly compared. The reactivity of the nucleophile, reaction conditions (solvent, buffer, pH) and linker length all play important roles in determining the efficiency of the transfer reaction. Careful optimization of the system enables the use of DNA-templated synthesis to form stable peptide-like bonds under mild aqueous conditions close to neutral pH. The experimental process involved the reaction of Bis(2,5-dioxopyrrolidin-1-yl) glutarate(cas: 79642-50-5).Name: Bis(2,5-dioxopyrrolidin-1-yl) glutarate

The Article related to peptidomimetic bond formation dna templated acyl transfer, Carbohydrates: Nucleic Acid Chemical Synthesis and other aspects.Name: Bis(2,5-dioxopyrrolidin-1-yl) glutarate

Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics

On May 1, 2016, Kowata, Keiko; Kojima, Naoshi; Komatsu, Yasuo published an article.Application of 79642-50-5 The title of the article was Development of a 3′-amino linker with high conjugation activity and its application to conveniently cross-link blunt ends of a duplex. And the article contained the following:

The 2-aminoethyl carbamate linker (ssH linker) exhibits high activity in modifying the 5′-termini of oligonucleotides; however, the ssH linker is not appropriate for 3′-terminal modification because it undergoes intramol. trans-acylation under heat-aqueous ammonia conditions. We developed an N-(2-aminoethyl)carbamate linker (revH linker), in which the carbamate is oriented in the reverse direction relative to that in 2-aminoethyl carbamate. The revH linker was tolerant to heat-alk. conditions and retained its high reactivity in conjugation with exogenous mols. The 3′-revH linker was efficiently linked with the 5′-ssH linker at the termini of complementary double strands with a bifunctional mol., producing a synthetic loop structure. An anti-microRNA oligonucleotide (AMO) was prepared from the chem. ligation of three-stranded 2′-O-Me RNAs, and the AMO with two alkyl loops exhibited high inhibition activity toward miRNA function. The revH linker is not only useful for 3′-terminal modification of oligonucleotides but also expands the utility range in combination with the 5′-ssH linker. The experimental process involved the reaction of Bis(2,5-dioxopyrrolidin-1-yl) glutarate(cas: 79642-50-5).Application of 79642-50-5

The Article related to microrna dna duplex preparation aminoethylcarbamate linker ligation, amino linker, cross-link, labeling, nucleic acids, oligonucleotides, anti-microrna, Carbohydrates: Nucleic Acid Chemical Synthesis and other aspects.Application of 79642-50-5

Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics

On March 31, 2011, Santos, Luiz Fernando Arruda; Eberlin, Marcos Nogueira; Gozzo, Fabio Cesar published an article.Reference of Bis(2,5-dioxopyrrolidin-1-yl) glutarate The title of the article was IRMPD and ECD fragmentation of intermolecular cross-linked peptides. And the article contained the following:

Despite the increasing number of studies using mass spectrometry for three dimensional analyses of proteins (MS3D), the identification of crosslinked peptides remains a bottleneck of the method. One of the main reasons for this is the lack of knowledge about the fragmentation of these species. Intermol. crosslinked peptides are considered the most informative species present in MS3D experiment, since different peptides are connected by a crosslinker, the peptides chain can be either from a single protein, providing information about protein folding, or from two different proteins in a complex, providing information about binding partners, complex topol. and interaction sites. These species tend to be large and highly charged in ESI, making comprehensive fragmentation by CID MS/MS problematic. On the other hand, these highly charged peptides are very suitable for dissociation using both IR multiphoton dissociation (IRMPD) and electron capture dissociation (ECD). Herein, we report the fragmentation study of intermol. crosslinked peptides using IRMPD and ECD. Using synthetic peptides and different com. crosslinkers, a series of intermol. crosslinked peptides were generate, and subsequently fragmented by IRMPD and ECD in a FT-ICR-MS instrument. Due to the high mass accuracy and resolution of the FT-ICR, the fragment ions could be attributed with high confidence. The peptides sequence coverage and fragmentation features obtained from IRMPD and ECD were compared for all charge states. The experimental process involved the reaction of Bis(2,5-dioxopyrrolidin-1-yl) glutarate(cas: 79642-50-5).Reference of Bis(2,5-dioxopyrrolidin-1-yl) glutarate

The Article related to irmpd ecd fragmentation intermol cross linked peptide ms, Biochemical Methods: Spectral and Related Methods and other aspects.Reference of Bis(2,5-dioxopyrrolidin-1-yl) glutarate

Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics

On August 17, 2016, Pagoto, Amerigo; Stefania, Rachele; Garello, Francesca; Arena, Francesca; Digilio, Giuseppe; Aime, Silvio; Terreno, Enzo published an article.HPLC of Formula: 79642-50-5 The title of the article was Paramagnetic Phospholipid-Based Micelles Targeting VCAM-1 Receptors for MRI Visualization of Inflammation. And the article contained the following:

Inflammation is signaled by the overexpression of epitopes on the vascular endothelium that primarily aim at recruiting immune cells into the inflamed area. The intravascular localization of these biomarkers makes them suitable targets for the MRI visualization of inflammation. Phospholipid-based nanosystems appear excellent candidates in virtue of their good biocompatibility, ability to deliver a high number of imaging units at the target site, and for the easy functionalization with targeting vectors. In this work, phospholipid-based micelles (hydrodynamic diameter of 20 nm) loaded with the amphiphilic Gd(III)-complex Gd-DOTAMA(C18)2 were vectorized with a small peptide able to specifically bind VCAM-1 receptors. The micelles displayed a high longitudinal relaxivity (36.4 s-1mmolGd-1 at 25 °C and 0.7 T). A 1H- and 17O-water relaxometry study indicated that the paramagnetic complex embedded in the nanoparticles adopted two isomeric conformations, likely reflecting the well-known square antiprismatic (SAP) and twisted square antiprismatic (TSAP) configurations typically observed in DOTA-like lanthanide complexes. Interestingly, the TSAP structure, showing a much faster exchange rate for the water mol. coordinated to the metal ion, was the most abundant, thus explaining the high relaxivity of the micellar agent. The systemic administration of the micelles into a lipopolysaccharide-induced murine model of acute inflammation successfully demonstrated the ability of the targeting agents to detect the diseased area by T1 contrast enhanced MRI. The experimental process involved the reaction of Bis(2,5-dioxopyrrolidin-1-yl) glutarate(cas: 79642-50-5).HPLC of Formula: 79642-50-5

The Article related to paramagnetic phospholipid micelle vcam1 receptor mri inflammation, Biochemical Methods: Spectral and Related Methods and other aspects.HPLC of Formula: 79642-50-5

Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics

On September 30, 2017, Stojko, Johann; Dupin, Adrien; Chaignepain, Stephane; Beaurepaire, Lionel; Vallet-Courbin, Amelie; Van Dorsselaer, Alain; Schmitter, Jean-Marie; Minvielle-Sebastia, Lionel; Fribourg, Sebastien; Cianferani, Sarah published an article.HPLC of Formula: 79642-50-5 The title of the article was Structural characterization of the yeast CF IA complex through a combination of mass spectrometry approaches. And the article contained the following:

The cleavage/polyadenylation factor IA (CF IA) is a yeast multiprotein complex that consists of Rna14, Rna15, Pcf11 and Clp1 proteins, and is involved in the 3′-end maturation of mRNAs. Structural data have been reported for the individual protein partners and binary complexes; however, little is known about the mol. architecture of the entire CF IA assembly. Here, we report a thorough characterization of complete recombinant CF IA assembly and its subcomplexes using a combination of mass spectrometry (MS) approaches. We first focused on the Rna14p:Rna15p and Pcf11p:Clp1p subcomplexes in order to obtain a detailed picture of their interactions. Native MS and crosslinking MS showed that the intact CF IA assembly exists in solution as pentameric and hexameric species, composed of two copies of Rna14p, one each of Pcf11p and Clp1p, and one or two of Rna15p, resp. Partial denaturation experiments followed by native MS along with crosslinking anal. revealed two building blocks: Rna14p:Rna15p multimer subcomplexes assemble with Pcf11p:Clp1p heterodimers to form the CF IA complex. We then used ion mobility-MS (IM-MS) to investigate the conformational changes induced upon CF IA assembly. The new information on the CF IA assembly process provided by this combination of MS approaches (native MS, crosslinking MS and IM-MS) allowed us to discuss a topol. model of the CF IA assembly. The experimental process involved the reaction of Bis(2,5-dioxopyrrolidin-1-yl) glutarate(cas: 79642-50-5).HPLC of Formula: 79642-50-5

The Article related to cleavage polyadenylation factor protein yeast ion mobility crosslinking, Biochemical Methods: Spectral and Related Methods and other aspects.HPLC of Formula: 79642-50-5

Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics

On April 5, 2011, Mealman, Tiffany D.; Bagai, Ireena; Singh, Pragya; Goodlett, David R.; Rensing, Christopher; Zhou, Hongjun; Wysocki, Vicki H.; McEvoy, Megan M. published an article.Reference of Bis(2,5-dioxopyrrolidin-1-yl) glutarate The title of the article was Interactions between CusF and CusB Identified by NMR Spectroscopy and Chemical Cross-Linking Coupled to Mass Spectrometry. And the article contained the following:

The Escherichia coli periplasmic proteins CusF and CusB, as part of the CusCFBA efflux system, aid in the resistance of elevated levels of copper and silver by direct metal transfer between the metallochaperone CusF and the membrane fusion protein CusB before metal extrusion from the periplasm to the extracellular space. Although previous in vitro experiments have demonstrated highly specific interactions between CusF and CusB that are crucial for metal transfer to occur, the structural details of the interaction have not been determined Here, the interactions between CusF and CusB are mapped through NMR spectroscopy and chem. crosslinking coupled with high-resolution mass spectrometry to better understand how recognition and metal transfer occur between these proteins. The NMR 1H-15N correlation spectra reveal that CusB interacts with the metal-binding face of CusF. In vitro chem. crosslinking with a 7.7 Å homobifunctional amine-reactive cross-linker, BS2G, was used to capture the CusF/CusB interaction site, and mass spectral data acquired on an LTQ-Orbitrap confirm the following two cross-links: CusF K31 to CusB K29 and CusF K58 to CusB K32, thus revealing that the N-terminal region of CusB interacts with the metal-binding face of CusF. The proteins transiently interact in a metal-dependent fashion, and contacts between CusF and CusB are localized to regions near their resp. metal-binding sites. The experimental process involved the reaction of Bis(2,5-dioxopyrrolidin-1-yl) glutarate(cas: 79642-50-5).Reference of Bis(2,5-dioxopyrrolidin-1-yl) glutarate

The Article related to interaction cusf cusb nmr spectroscopy crosslinking coupled mass spectrometry, Biochemical Methods: Spectral and Related Methods and other aspects.Reference of Bis(2,5-dioxopyrrolidin-1-yl) glutarate

Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics

Natrajan, Anand; Wen, David published an article in 2015, the title of the article was A comparison of chemiluminescent acridinium dimethylphenyl ester labels with different conjugation sites.Reference of Bis(2,5-dioxopyrrolidin-1-yl) glutarate And the article contains the following content:

Chemiluminescent acridinium dimethylphenyl esters are highly sensitive labels that were used in automated assays for clin. diagnosis. Light emission from these labels and their conjugates is triggered by treatment with alk. peroxide. Conjugation of acridinium ester labels is normally done at the phenol. During the chemiluminescent reaction of these acridinium esters, the phenolic ester is cleaved and the light emitting acridone moiety is liberated from its conjugate partner. In the current study, the authors report the synthesis of three new acridinium esters with conjugation sites at the acridinium nitrogen and compare their properties with that of a conventional acridinium ester with a conjugation site at the phenol. The authors’ study is the first that provides a direct comparison of the emissive properties of acridinium dimethylphenyl esters (free labels and protein conjugates) with different conjugation sites, one where the light emitting acridone remains attached to its conjugate partner vs. conventional labeling which results in cleavage of the acridone from the conjugate. The authors’ results indicate that the conjugation at the acridinium nitrogen, which also alters how the acridinium ring and phenol are oriented with respect to the protein surface, has a minimal impact on emission kinetics and emission spectra. However, this mode of conjugation to three different proteins led to a significant increase in light yield which should be useful for improving the assay sensitivity. The experimental process involved the reaction of Bis(2,5-dioxopyrrolidin-1-yl) glutarate(cas: 79642-50-5).Reference of Bis(2,5-dioxopyrrolidin-1-yl) glutarate

The Article related to chemiluminescent acridinium dimethylphenyl ester label conjugation site protein, Biochemical Methods: Spectral and Related Methods and other aspects.Reference of Bis(2,5-dioxopyrrolidin-1-yl) glutarate

Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics

On December 31, 2017, Afroz, Tariq; Hock, Eva-Maria; Ernst, Patrick; Foglieni, Chiara; Jambeau, Melanie; Gilhespy, Larissa A. B.; Laferriere, Florent; Maniecka, Zuzanna; Pluckthun, Andreas; Mittl, Peer; Paganetti, Paolo; Allain, Frederic H. T.; Polymenidou, Magdalini published an article.Synthetic Route of 79642-50-5 The title of the article was Functional and dynamic polymerization of the ALS-linked protein TDP-43 antagonizes its pathologic aggregation. And the article contained the following:

TDP-43 is a primarily nuclear RNA-binding protein, whose abnormal phosphorylation and cytoplasmic aggregation characterizes affected neurons in patients with amyotrophic lateral sclerosis and frontotemporal dementia. Here, we report that physiol. nuclear TDP-43 in mouse and human brain forms homo-oligomers that are resistant to cellular stress. Physiol. TDP-43 oligomerization is mediated by its N-terminal domain, which can adopt dynamic, solenoid-like structures, as revealed by a 2.1 Å crystal structure in combination with NMR spectroscopy and electron microscopy. These head-to-tail TDP-43 oligomers are unique among known RNA-binding proteins and represent the functional form of the protein in vivo, since their destabilization results in loss of alternative splicing regulation of known neuronal RNA targets. Our findings indicate that N-terminal domain-driven oligomerization spatially separates the adjoining highly aggregation-prone, C-terminal low-complexity domains of consecutive TDP-43 monomers, thereby preventing low-complexity domain inter-mol. interactions and antagonizing the formation of pathol. aggregates. The experimental process involved the reaction of Bis(2,5-dioxopyrrolidin-1-yl) glutarate(cas: 79642-50-5).Synthetic Route of 79642-50-5

The Article related to tdp43 als rna protein structure brain, General Biochemistry: Proteins and Their Constituents and other aspects.Synthetic Route of 79642-50-5

Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics

On March 1, 2013, Dettmer, Ulf; Newman, Andrew J.; Luth, Eric S.; Bartels, Tim; Selkoe, Dennis published an article.Related Products of 79642-50-5 The title of the article was In Vivo Cross-linking Reveals Principally Oligomeric Forms of α-Synuclein and β-Synuclein in Neurons and Non-neural Cells. And the article contained the following:

Aggregation of α-synuclein (αSyn) in neurons produces the hallmark cytopathol. of Parkinson disease and related synucleinopathies. Since its discovery, αSyn has been thought to exist normally in cells as an unfolded monomer. It was recently reported that αSyn can instead exist in cells as a helically folded tetramer that resists aggregation and binds lipid vesicles more avidly than unfolded recombinant monomers. However, a subsequent study again concluded that cellular αSyn is an unfolded monomer. Here a simple in vivo crosslinking method is described that reveals a major ∼60-kDa form of endogenous αSyn monomer, 14.5 kDa in intact cells and smaller amounts of ∼80- and ∼100-kDa forms with the same isoelec. point as the 60-kDa species. Controls indicate that the apparent 60-kDa tetramer exists normally and does not arise from pathol. aggregation. The pattern of a major 60-kDa and minor 80- and 100-kDa species plus variable amounts of free monomers occurs endogenously in primary neurons and erythroid cells as well as neuroblastoma cells over-expressing αSyn. A similar pattern occurs for the homolog, β-synuclein, which does not undergo pathogenic aggregation. Cell lysis destabilizes the apparent 60-kDa tetramer, leaving mostly free monomers and some 80-kDa oligomer. However, lysis at high protein concentrations allows partial recovery of the 60-kDa tetramer. Together with the prior findings, these data suggest that endogenous αSyn exists principally as a 60-kDa tetramer in living cells but is lysis-sensitive, making the study of natural αSyn challenging outside of intact cells. The experimental process involved the reaction of Bis(2,5-dioxopyrrolidin-1-yl) glutarate(cas: 79642-50-5).Related Products of 79642-50-5

The Article related to endogenous quaternary structure alpha beta synuclein oligomer crosslinking, General Biochemistry: Proteins and Their Constituents and other aspects.Related Products of 79642-50-5

Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics

On February 6, 2018, Obata, Shunsuke; Asamitsu, Sefan; Hashiya, Kaori; Bando, Toshikazu; Sugiyama, Hiroshi published an article.Formula: C13H14N2O8 The title of the article was G-Quadruplex Induction by the Hairpin Pyrrole-Imidazole Polyamide Dimer. And the article contained the following:

The G-quadruplex (G4) is one type of higher-order structure of nucleic acids and is thought to play important roles in various biol. events such as regulation of transcription and inhibition of DNA replication. Pyrrole-imidazole polyamides (PIPs) are programmable small mols. that can sequence-specifically bind with high affinity to the minor groove of double-stranded DNA (dsDNA). Herein, we designed head-to-head hairpin PIP dimers and their target dsDNA in a model G4-forming sequence. Using an electrophoresis mobility shift assay and transcription arrest assay, we found that PIP dimers could induce the structural change to G4 DNA from dsDNA through the recognition by one PIP dimer mol. of two duplex-binding sites flanking both ends of the G4-forming sequence. This induction ability was dependent on linker length. This is the first study to induce G4 formation using PIPs, which are known to be dsDNA binders. The results reported here suggest that selective G4 induction in native sequences may be achieved with PIP dimers by applying the same design strategy. The experimental process involved the reaction of Bis(2,5-dioxopyrrolidin-1-yl) glutarate(cas: 79642-50-5).Formula: C13H14N2O8

The Article related to hairpin pyrrole imidazole polyamide dimer dna transition g quadruplex, General Biochemistry: Nucleic Acids and Their Constituents and other aspects.Formula: C13H14N2O8

Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics