Category: 79642-50-5

Kim, Jee Young; Sung, Gun Yong; Park, Min published an article in 2020, the title of the article was Efficient portable urea biosensor based on urease immobilized membrane for monitoring of physiological fluids.Recommanded Product: Bis(2,5-dioxopyrrolidin-1-yl) glutarate And the article contains the following content:

Herein, a portable urea biosensor was developed for the real-time monitoring of the flow of physiol. fluids; this was achieved by using disuccinimidyl cross-linker-based urease immobilization. Urease was immobilized on a porous polytetrafluoroethylene (PTFE) solid support using different disuccinimidyl cross-linkers, namely disuccinimidyl glutarate (DSG), disuccinimidyl suberate (DSS) and bis-N-succinimidyl-(pentaethylene glycol) ester (BS(PEG)5). A urease activity test revealed that DSS exhibited the highest urease immobilizing efficiency, whereas FT-IR anal. confirmed that urease was immobilized on the PTFE membrane via DSS crosslinking. The membrane was inserted in a polydimethylsiloxane (PDMS) fluidic chamber that generated an electrochem. signal in the presence of a flowing fluid containing urea. Urea samples were allowed to flow into the urea biosensor (1.0 mL/min) and the signal was measured using chronoamperometry. The sensitivity of the DSS urea biosensor was the highest of all the trialed biosensors and was found to be superior to the more commonly used GA cross-linker. To simulate real-time monitoring in a human patient, flowing urea-spiked human serum was measured and the effective urease immobilization of the DSS urea biosensor was confirmed. The repeatability and interference of the urea biosensor were suitable for monitoring urea concentration typically found in human patients. The experimental process involved the reaction of Bis(2,5-dioxopyrrolidin-1-yl) glutarate(cas: 79642-50-5).Recommanded Product: Bis(2,5-dioxopyrrolidin-1-yl) glutarate

The Article related to body fluid urease urea biosensor, disuccinimidyl cross-linker, flow system, real-time monitoring, urea biosensor, urease immobilization, Biochemical Methods: Biological and other aspects.Recommanded Product: Bis(2,5-dioxopyrrolidin-1-yl) glutarate

Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics

Beneke, Sascha published an article in 2015, the title of the article was Improving Chromatin Immunoprecipitation (ChIP) by Suppression of Method-Induced DNA-Damage Signaling.Category: esters-buliding-blocks And the article contains the following content:

Genomic DNA is always associated with proteins that modulate the accessibility of the genetic information. This chromatin is the essential structure in which all nuclear activity from regulation to replication, transcription, and repair takes place. This dynamic structure can be most efficiently analyzed by using the method of chromatin immunoprecipitation (ChIP), where application of cell-permeable cross-linkers to living cells induces covalent bridging between proteins and adjacent DNA in the nucleus. After fragmentation of the DNA, the complexed proteins are isolated by binding to specific antibodies. The attached DNA is isolated and can be analyzed. This method has been improved multiple times and adjusted to different exptl. needs. This chapter describes a further advance based on the observation that the current standard method itself induces alterations in the chromatin. The experimental process involved the reaction of Bis(2,5-dioxopyrrolidin-1-yl) glutarate(cas: 79642-50-5).Category: esters-buliding-blocks

The Article related to chromatin immunoprecipitation dna damage signaling, Biochemical Methods: Immunological and other aspects.Category: esters-buliding-blocks

Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics

On December 19, 2017, Je, Goun; Croop, Benjamin; Basu, Sambuddha; Tang, Jialei; Han, Kyu Young; Kim, Yoon-Seong published an article.Quality Control of Bis(2,5-dioxopyrrolidin-1-yl) glutarate The title of the article was Endogenous Alpha-Synuclein Protein Analysis from Human Brain Tissues Using Single-Molecule Pull-Down Assay. And the article contained the following:

Alpha-synuclein (α-SYN) is a central mol. in Parkinson’s disease pathogenesis. Despite several studies, the mol. nature of endogenous α-SYN especially in human brain samples is still not well understood due to the lack of reliable methods and the limited amount of biospecimens. Here, we introduce α-SYN single-mol. pull-down (α-SYN SiMPull) assay combined with in vivo protein crosslinking to count individual α-SYN protein and assess its native oligomerization states from biol. samples including human postmortem brains. This powerful single-mol. assay can be highly useful in diagnostic applications using various specimens for neurodegenerative diseases including Alzheimer’s disease and Parkinson’s disease. The experimental process involved the reaction of Bis(2,5-dioxopyrrolidin-1-yl) glutarate(cas: 79642-50-5).Quality Control of Bis(2,5-dioxopyrrolidin-1-yl) glutarate

The Article related to single mol pull down assay alpha synuclein brain human, Biochemical Methods: Immunological and other aspects.Quality Control of Bis(2,5-dioxopyrrolidin-1-yl) glutarate

Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics

Natrajan, Anand; Sharpe, David; Wen, David published an article in 2012, the title of the article was Zwitterionic reagents for labeling, cross-linking and improving the performance of chemiluminescent immunoassays.COA of Formula: C13H14N2O8 And the article contains the following content:

Improving reagent performance in immunoassays both to enhance assay sensitivity and to minimize interference are ongoing challenges in clin. diagnostics. The authors describe herein the syntheses of a new class of hydrophilic reagents containing sulfobetaine zwitterions and their applications. These zwitterionic reagents are potentially useful for improving the properties of immunoassay reagents. The authors demonstrate for the first time that zwitterion labeling is a general and viable strategy for reducing the nonspecific binding of proteins to microparticles and, to improve the aqueous solubility of hydrophobic peptides. The authors also describe the synthesis of zwitterionic crosslinking reagents and demonstrate their utility for peptide conjugation. In automated, chemiluminescent immunoassays, improved assay performance was observed for a hydrophobic, small analyte (theophylline) using an acridinium ester conjugate with a zwitterionic sulfobetaine linker compared to a hexa(ethylene)glycol linker. Sandwich assay performance for a large analyte (TSH) was similar for the two acridinium ester labels. These results indicate that zwitterions are complementary to poly(ethylene)glycol in improving the aqueous solubility and reducing the nonspecific binding of chemiluminescent acridinium ester conjugates. The experimental process involved the reaction of Bis(2,5-dioxopyrrolidin-1-yl) glutarate(cas: 79642-50-5).COA of Formula: C13H14N2O8

The Article related to zwitterionic reagent labeling crosslinking performance chemiluminescent immunoassay, Biochemical Methods: Immunological and other aspects.COA of Formula: C13H14N2O8

Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics

On June 14, 2012, Richards, David N.; Zemlyanov, Dmitry Y.; Ivanisevic, Albena published an article.Electric Literature of 79642-50-5 The title of the article was Kelvin Probe Force Microscopy Analysis of the Covalent Functionalization and DNA Modification of Gallium Phosphide Nanorods. And the article contained the following:

The growth, covalent functionalization, and subsequent DNA modification of Ga phosphide (GaP) nanorods is presented. Anal. of the nanorods by SEM, TEM, and XRD revealed important information regarding their phys. properties such as the presence of twinning defects. The nanorods were deposited onto glass substrates for further functionalization and biomol. immobilization. Plasma cleaning was employed to remove the surfactant present on the nanorods’ surfaces. Kelvin probe force microscopy (KPFM) was used to analyze the extent of plasma cleaning and how it affected the functionalization that employed thiol chem. KFPM anal. of the subsequent modification of functionalized nanorods with single-stranded DNA (ssDNA) revealed that immobilization was dependent on the amount of plasma cleaning to which the nanorods had been exposed. Nanorods were then exposed to the cDNA strand and KPFM was again used to detect successful hybridization. The experimental process involved the reaction of Bis(2,5-dioxopyrrolidin-1-yl) glutarate(cas: 79642-50-5).Electric Literature of 79642-50-5

The Article related to kelvin probe microscopy dna modification gallium phosphide nanorod, Surface Chemistry and Colloids: Other and other aspects.Electric Literature of 79642-50-5

Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics

On December 31, 2019, Sachs, Parysatis; Ding, Dong; Bergmaier, Philipp; Lamp, Boris; Schlagheck, Christina; Finkernagel, Florian; Nist, Andrea; Stiewe, Thorsten; Mermoud, Jacqueline E. published an article.Recommanded Product: Bis(2,5-dioxopyrrolidin-1-yl) glutarate The title of the article was SMARCAD1 ATPase activity is required to silence endogenous retroviruses in embryonic stem cells. And the article contained the following:

Endogenous retroviruses (ERVs) can confer benefits to their host but present a threat to genome integrity if not regulated correctly. Here we identify the SWI/SNF-like remodeler SMARCAD1 as a key factor in the control of ERVs in embryonic stem cells. SMARCAD1 is enriched at ERV subfamilies class I and II, particularly at active intracisternal A-type particles (IAPs), where it preserves repressive histone methylation marks. Depletion of SMARCAD1 results in de-repression of IAPs and adjacent genes. Recruitment of SMARCAD1 to ERVs is dependent on KAP1, a central component of the silencing machinery. SMARCAD1 and KAP1 occupancy at ERVs is co-dependent and requires the ATPase function of SMARCAD1. Our findings uncover a role for the enzymic activity of SMARCAD1 in cooperating with KAP1 to silence ERVs. This reveals ATP-dependent chromatin remodeling as an integral step in retrotransposon regulation in stem cells and advances our understanding of the mechanisms driving heterochromatin establishment. The experimental process involved the reaction of Bis(2,5-dioxopyrrolidin-1-yl) glutarate(cas: 79642-50-5).Recommanded Product: Bis(2,5-dioxopyrrolidin-1-yl) glutarate

The Article related to retrovirus embryonic stem cell smarcad1, Biochemical Genetics: Genomic Processes and other aspects.Recommanded Product: Bis(2,5-dioxopyrrolidin-1-yl) glutarate

Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics

Romano, Simona; D′Arrigo, Paolo; Tufano, Martina; Staibano, Stefania; Rea, Anna; Merolla, Francesco; Ilardi, Gennaro; Petrella, Antonello; Romano, Maria F. published an article in 2019, the title of the article was TRAF2 and FKBP51 as possible markers for identification of suitable melanoma tumors for tumor necrosis factor-α inhibition.Formula: C13H14N2O8 And the article contains the following content:

Tumor necrosis factor-α (TNF-α) is a pleiotropic cytokine, whose role in melanoma is controversial. Although high-dose TNF-α is approved for the treatment of patients with in transit-metastatic melanoma confined to the limb, diverse preclin. models of melanoma have shown that TNF-α can induce cell invasion. Biomarkers that can differentiate between the dual role of TNF-α are needed. TRAF2 is critical to TNF receptor-induced activation of nuclear factor-κB (NF-κB), allowing shifting from death to survival-signaling cascades. The large immunophilin FKBP51 acts as a scaffold and catalyst in the IκB kinase complex assembly and activation. Here, using microscopy and an electrophoretic mobility-shift assay, we provide further evidence in support of the essential role of FKBP51 in sustaining the TNF-α NF-κB signaling in melanoma. Through the crosslinking reaction with the chem. linker disuccinimidyl glutarate, we show that a direct interaction occurs between FKBP51 and TRAF2 in melanoma cells. Immunohistochem. of tumor samples from 24 patients with cutaneous melanomas showed a correlation between the expressions of the two proteins. Given the association of FKBP51 and TRAF2 with TNF-α-induced NF-κB signaling and their correlation in tumor samples, we propose that the two proteins can be exploited as useful markers for the identification of those melanoma tumors that can benefit from TNF-α inhibition. Future studies will address this hypothesis. The experimental process involved the reaction of Bis(2,5-dioxopyrrolidin-1-yl) glutarate(cas: 79642-50-5).Formula: C13H14N2O8

The Article related to melanoma tumor traf2 fkbp51 biomarker tnfa, Placeholder for records without volume info and other aspects.Formula: C13H14N2O8

Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics

On September 30, 2022, Liao, Jun; Pan, Bo; Zhuo, Xiaobin; Liao, Guochao; Gao, Yun; Yao, Zhenzhen; Wang, Lianghua; Wu, Qiuye; Pan, Weihua; Jiao, Binghua; Zhao, Qingjie published an article.Computed Properties of 79642-50-5 The title of the article was β-1,2-Mannan-based glycoconjugates as potential antifungal vaccines. And the article contained the following:

Phosphorylated di-, tri- and tetra-saccharides of β-1,2-mannan antigen derived from Candida albicans (C. albicans) cell wall were synthesized and covalently conjugated with keyhole limpet hemocyanin (KLH) and human serum albumin (HSA) via a bifunctional linker under mild conditions. The semi-synthetic β-1,2-mannoside-KLH conjugates were evaluated for the immunization of BALB/c mice. The ELISA results revealed that all three conjugates could elicit high levels of specific IgG antibodies and the acquired antisera could effectively identify the β-1,2-mannan epitope. Furthermore, the immunofluorescence and flow cytometry assays also uncovered that the induced antibodies, especially that obtained from immunization with β-1,2-mannotriose-KLH conjugate (1b), could bind well to fungi cell. Eventually, the structure-immunogenicity relationship anal. of β-mannan showed that the length of oligo-β-mannoses had a big impact on their immunogenicity and β-1,2-mannotriose showed the strongest immunogenicity. The results suggested the great potential of β-1,2-mannotriose-KLH conjugate as an antifungal vaccine candidate. The experimental process involved the reaction of Bis(2,5-dioxopyrrolidin-1-yl) glutarate(cas: 79642-50-5).Computed Properties of 79642-50-5

The Article related to candida beta mannan glycoconjugate antifungal vaccine, Placeholder for records without volume info and other aspects.Computed Properties of 79642-50-5

Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics

Parrish, Emmabeth; Rose, Katie A.; Cargnello, Matteo; Murray, Christopher B.; Lee, Daeyeon; Composto, Russell J. published an article in 2020, the title of the article was Nanoparticle diffusion during gelation of tetra poly(ethylene glycol) provides insight into nanoscale structural evolution.Category: esters-buliding-blocks And the article contains the following content:

Single particle tracking (SPT) of PEG grafted nanoparticles (NPs) was used to examine the gelation of tetra poly(ethylene glycol) (TPEG) succinimidyl glutarate (TPEG-SG) and amine (TPEG-A) terminated 4-armed stars. As concentration was decreased from 40 to 20 mg mL-1, the onset of network formation, tgel, determined from rheometry increased from less than 2 to 44 min. NP mobility increased as polymer concentration decreased in the sol state, but remained diffusive at times past the tgel determined from rheometry. Once in the gel state, NP mobility decreased, became sub-diffusive, and eventually localized in all concentrations The NP displacement distributions were investigated to gain insight into the nanoscale environment. In these relatively homogeneous gels, the onset of sub-diffusivity was marked by a rapid increase in dynamic heterogeneity followed by a decrease consistent with a homogeneous network. We propose a gelation mechanism in which clusters initially form a heterogeneous structure which fills in to form a fully gelled relatively homogenous network. This work aims to examine the kinetics of TPEG gelation and the homogeneity of these novel gels on the nanometer scale, which will aid in the implementation of these gels in biomedical or filtration applications. The experimental process involved the reaction of Bis(2,5-dioxopyrrolidin-1-yl) glutarate(cas: 79642-50-5).Category: esters-buliding-blocks

The Article related to tetra polyethylene glycol nanoparticle diffusion gelation property, Placeholder for records without volume info and other aspects.Category: esters-buliding-blocks

Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics

On December 31, 2022, Ma, Shenghong; Tang, Tracy; Probst, Gary; Konradi, Andrei; Jin, Chunyu; Li, Fulong; Silvio Gutkind, J.; Fu, Xiang-Dong; Guan, Kun-Liang published an article.Quality Control of Bis(2,5-dioxopyrrolidin-1-yl) glutarate The title of the article was Transcriptional repression of estrogen receptor alpha by YAP reveals the Hippo pathway as therapeutic target for ER+ breast cancer. And the article contained the following:

Extensive knowledge has been gained on the transcription network controlled by ERα, however, the mechanism underlying ESR1 (encoding ERα) expression is less understood. We recently discovered that the Hippo pathway is required for the proper expression of ESR1. YAP/TAZ are transcription coactivators that are phosphorylated and inhibited by the Hippo pathway kinase LATS. Here we delineated the mol. mechanisms underlying ESR1 transcription repression by the Hippo pathway. Mechanistically, YAP binds to TEAD to increase local chromatin accessibility to stimulate transcription of nearby genes. Among the YAP target genes, Vestigial-Like Protein 3 (VGLL3) competes with YAP/TAZ for binding to TEAD transcription factor and recruits the NCOR2/SMRT repressor to the super-enhancer of ESR1 gene, leading to epigenetic alteration and transcriptional silencing. We developed a potent LATS inhibitor VT02956. Targeting the Hippo pathway by VT02956 represses ESR1 expression and inhibits the growth of ER+ breast cancer cells as well as patient-derived tumor organoids. Moreover, histone deacetylase inhibitors, such as Entinostat, induce VGLL3 expression to inhibit ER+ breast cancer cells. Our study suggests LATS as unexpected cancer therapeutic targets, especially for endocrine-resistant breast cancers. The experimental process involved the reaction of Bis(2,5-dioxopyrrolidin-1-yl) glutarate(cas: 79642-50-5).Quality Control of Bis(2,5-dioxopyrrolidin-1-yl) glutarate

The Article related to esr alpha therapeutic target er breast cancer transcriptional repression, Placeholder for records without volume info and other aspects.Quality Control of Bis(2,5-dioxopyrrolidin-1-yl) glutarate

Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics