Category: 79642-50-5

Rasmussen, Kasper D.; Helin, Kristian published an article in 2021, the title of the article was ChIP-Sequencing of TET ProteinsTET proteins.Computed Properties of 79642-50-5 And the article contains the following content:

A review. TET proteins are methylcytosine dioxygenases that interact directly with chromatin to shape the DNA methylation landscape. To increase the understanding of TET protein function in a specific cellular context, it is important to be able to map the interactions between TET proteins and DNA. This ChIP-seq protocol details our procedure to analyze TET2 bound DNA in disuccinimidyl glutarate (DSG) and formaldehyde-crosslinked chromatin but can also be adapted to study other TET enzymes. The experimental process involved the reaction of Bis(2,5-dioxopyrrolidin-1-yl) glutarate(cas: 79642-50-5).Computed Properties of 79642-50-5

The Article related to review sequencing protein disuccinimidyl glutarate formaldehyde, chip-seq, dna methylation, dsg, tet1, tet2, tet3, Biochemical Methods: Reviews and other aspects.Computed Properties of 79642-50-5

Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics

On December 31, 2016, Hsieh, Tsung-Han S.; Fudenberg, Geoffrey; Goloborodko, Anton; Rando, Oliver J. published an article.Application In Synthesis of Bis(2,5-dioxopyrrolidin-1-yl) glutarate The title of the article was Micro-C XL: assaying chromosome conformation from the nucleosome to the entire genome. And the article contained the following:

We present Micro-C XL, an improved method for anal. of chromosome folding at mononucleosome resolution Using long crosslinkers and isolation of insoluble chromatin, Micro-C XL increases signal-to-noise ratio. Micro-C XL maps of budding and fission yeast genomes capture both short-range chromosome fiber features such as chromosomally interacting domains and higher order features such as centromere clustering. Micro-C XL provides a single assay to interrogate chromosome folding at length scales from the nucleosome to the full genome. The experimental process involved the reaction of Bis(2,5-dioxopyrrolidin-1-yl) glutarate(cas: 79642-50-5).Application In Synthesis of Bis(2,5-dioxopyrrolidin-1-yl) glutarate

The Article related to chromosome folding nucleosome dna conformation micro c xl, Biochemical Genetics: Methods and other aspects.Application In Synthesis of Bis(2,5-dioxopyrrolidin-1-yl) glutarate

Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics

Bian, Shudan; He, Jiajun; Schesing, Kevin B.; Braunschweig, Adam B. published an article in 2012, the title of the article was Polymer pen lithography (PPL)-induced site-specific click chemistry for the formation of functional glycan arrays.Electric Literature of 79642-50-5 And the article contains the following content:

PPL was combined successfully with the CuAAC to form functional mol. arrays over large (> cm2) areas and with feature diameters <1 μm. The problem of the differential transport rates of the reactive components through the aqueous meniscus was solved by embedding them within a PEG matrix. Covalent immobilization as a result of the CuAAC was demonstrated by fluorescence microscopy, and electrochem. anal. confirmed that the surface reactions produce features with high coverage d. Finally, the utility of this approach to glycomics was shown by creating an active array of α-mannose that could be recognized by ConA. In combination with previously reported multiplexing strategies, the authors believe that the approach reported herein could be employed widely to create arrays of biol. probes where the activity has a critical dependence on probe orientation. The experimental process involved the reaction of Bis(2,5-dioxopyrrolidin-1-yl) glutarate(cas: 79642-50-5).Electric Literature of 79642-50-5

The Article related to polymer pen lithog site specific click chem glycan microarray, Biochemical Methods: Apparatus and other aspects.Electric Literature of 79642-50-5

Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics

Watanabe, Rei; Hu, Yaping; Iio, Keita; Yoneda, Kozo; Hattori, Atsunori; Arai, Atsushi; Kigoshi, Hideo; Kita, Masaki published an article in 2018, the title of the article was Specific protein-labeling and ligand-binding position analysis with amidopyrene probes as LDI MS tags.Product Details of 79642-50-5 And the article contains the following content:

To readily analyze the binding mode of protein-ligand interactions, we developed ligand-bound-type and ligand-dissociation-type probes having 6-amidopyrene (apy) as a detecting group. Matrix- and label-assisted laser desorption/ionization mass spectrometry (MALDI and LA-LDI MS) analyses and a covalent docking simulation using these probes precisely determined the binding position of the ligand biotin on the target protein avidin (RMSD = 0.786 and 0.332 Å). Our apy-probe-labeling method may be useful for determining the unknown ligand-binding sites of various target proteins. The experimental process involved the reaction of Bis(2,5-dioxopyrrolidin-1-yl) glutarate(cas: 79642-50-5).Product Details of 79642-50-5

The Article related to protein labeling ligand interaction amidopyrene probe ldi mass spectrometry, Biochemical Methods: Synthesis and other aspects.Product Details of 79642-50-5

Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics

Aruna, Kasoju; Munawar, T. Mohammad; Prashanthi, M. V.; Narsu, M. Lakshmi published an article in 2013, the title of the article was Optimisation studies of probe DNA concentration on the surface of silica nanobiosensor.Quality Control of Bis(2,5-dioxopyrrolidin-1-yl) glutarate And the article contains the following content:

Biosensors with immobilized probe that are selective for the identification of toxic compounds at ultra trace levels in industrial products, chem. substances, environmental samples or biol. systems (e.g., Bacteria, virus, or tissue components) for biomedical diagnosis. Nanomaterials such as silica nanoparticles works as sensor when probe hybridized with the target sequence. Due to their large surface area to volume ratio, they can achieve rapid and low cost reactions. They can conjugate with a variety of biomols. (Proteins, enzymes, peptides, and DNA) and used as fluorescence biomarkers to monitor biol. events or identify and detect biol. targets. DNA biosensors based on nucleic acid hybridization were extensively developed because of their specificity, speed, portability, and low cost. The immobilization step is essential to ensure high reactivity, orientation, accessibility, and stability of the surface-confined probe and to avoid nonspecific binding. Various studies, i.e., a synthesis of silica nanoparticles, their surface modification, probe immobilization were carried out during optimization of reaction conditions. In this present work, 3.4% more immobilization of probe DNA onto the silica nanoparticle was observed in the presence of 10% butyltrimethoxysilane solution rather than absence of BMS and the concentration of the DNA probe immobilized on silica nanoparticles was maximum at 1Picomloles/μl was noticed. The experimental process involved the reaction of Bis(2,5-dioxopyrrolidin-1-yl) glutarate(cas: 79642-50-5).Quality Control of Bis(2,5-dioxopyrrolidin-1-yl) glutarate

The Article related to silica nanoparticle dna probe immobilization butyltrimethoxysilane biosensor, Biochemical Methods: Apparatus and other aspects.Quality Control of Bis(2,5-dioxopyrrolidin-1-yl) glutarate

Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics

On March 31, 2010, Pillai, R.; Marinelli, E. R.; Fan, H.; Nanjappan, P.; Song, B.; von Wronski, M. A.; Cherkaoui, S.; Tardy, I.; Pochon, S.; Schneider, M.; Nunn, A. D.; Swenson, R. E. published an article.Application In Synthesis of Bis(2,5-dioxopyrrolidin-1-yl) glutarate The title of the article was A Phospholipid-PEG2000 Conjugate of a Vascular Endothelial Growth Factor Receptor 2 (VEGFR2)-Targeting Heterodimer Peptide for Contrast-Enhanced Ultrasound Imaging of Angiogenesis. And the article contained the following:

The transition of a targeted ultrasound contrast agent from animal imaging to testing in clin. studies requires considerable chem. development. The nature of the construct changes from an agent that is chem. attached to microbubbles to one where the targeting group is coupled to a phospholipid, for direct incorporation to the bubble surface. We provide an efficient method to attach a heterodimeric peptide to a pegylated phospholipid and show that the resulting construct retains nanomolar affinity for its target, vascular endothelial growth factor receptor 2 (VEGFR2), for both the human (kinase insert domain-containing receptor – KDR) and the mouse (fetal liver kinase 1 – Flk-1) receptors. The purified phospholipid-PEG-peptide isolated from TFA-based eluents is not stable with respect to hydrolysis of the fatty ester moieties. This leads to the time-dependent formation of the lysophospholipid and the phosphoglycerylamide derived from the degradation of the product. Purification of the product using neutral eluent systems provides a stable product. Methods to prepare the lysophospholipid (hydrolysis product) are also included. Biacore binding data demonstrated the retention of binding of the lipopeptide to the KDR receptor. The phospholipid-PEG2000-peptide is smoothly incorporated into gas-filled microbubbles and provides imaging of angiogenesis in a rat tumor model. The experimental process involved the reaction of Bis(2,5-dioxopyrrolidin-1-yl) glutarate(cas: 79642-50-5).Application In Synthesis of Bis(2,5-dioxopyrrolidin-1-yl) glutarate

The Article related to preparation phospholipid polyethylene glycol conjugate contrast ultrasound imaging angiogenesis, Pharmaceuticals: Pharmaceutics and other aspects.Application In Synthesis of Bis(2,5-dioxopyrrolidin-1-yl) glutarate

Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics

On September 8, 2017, Cai, Deqin; Xun, Chaochao; Tang, Feng; Tian, Xiaobo; Yang, Liyun; Ding, Kan; Li, Wenzhe; Le, Zhiping; Huang, Wei published an article.Safety of Bis(2,5-dioxopyrrolidin-1-yl) glutarate The title of the article was Glycoconjugate probes containing a core-fucosylated N-glycan trisaccharide for fucose lectin identification and purification. And the article contained the following:

Glyco-PAMAM dendrimers and glyco-agarose beads bearing a core-fucosylated N-glycan trisaccharide GlcNAcβ1,4(Fucα1,6)GlcNAc or a nonfucose disaccharide GlcNAcβ1,4GlcNAc were successfully synthesized and characterized by monosaccharide anal. with HPAEC-PAD technique. These glycoconjugates as fucose lectin probes were applied in fucose-specific lectin detection and purification The model fucose lectin AAL indicated binding activity with the FITC-labeled PAMAM carrying core-fucose trisaccharide. An affinity chromatog. column stuffed with the agarose beads carrying core-fucosylated trisaccharide exhibited a good specificity in purification of AAL than nonfucose disaccharide agarose beads. These novel glycoconjugates bearing the precise and simplified core-fucose N-glycan structure provided a potential application for core-fucose-specific lectin discovery. The experimental process involved the reaction of Bis(2,5-dioxopyrrolidin-1-yl) glutarate(cas: 79642-50-5).Safety of Bis(2,5-dioxopyrrolidin-1-yl) glutarate

The Article related to glycoconjugate glycan trisaccharide fucose lectin, core-fucose, fucose-specific lectin, glyco-agarose, glyco-dendrimer, glycoconjugate, Biochemical Methods: Synthesis and other aspects.Safety of Bis(2,5-dioxopyrrolidin-1-yl) glutarate

Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics

On March 31, 2021, Nott, Alexi; Schlachetzki, Johannes C. M.; Fixsen, Bethany R.; Glass, Christopher K. published an article.Category: esters-buliding-blocks The title of the article was Nuclei isolation of multiple brain cell types for omics interrogation. And the article contained the following:

We present a nuclei isolation protocol for genomic and epigenomic interrogation of multiple cell type populations in the human and rodent brain. The nuclei isolation protocol allows cell type-specific profiling of neurons, microglia, oligodendrocytes, and astrocytes, being compatible with fresh and frozen samples obtained from either resected or postmortem brain tissue. This 2-day procedure consists of tissue homogenization with fixation, nuclei extraction, and antibody staining followed by fluorescence-activated nuclei sorting (FANS) and does not require specialized skillsets. Cell type-specific nuclei populations can be used for downstream omic-scale sequencing applications with an emphasis on epigenomic interrogation such as histone modifications, transcription factor binding, chromatin accessibility, and chromosome architecture. The nuclei isolation protocol enables translational examination of archived healthy and diseased brain specimens through utilization of existing medical biorepositories. The experimental process involved the reaction of Bis(2,5-dioxopyrrolidin-1-yl) glutarate(cas: 79642-50-5).Category: esters-buliding-blocks

The Article related to multiple brain cell type nuclei isolation omic interrogation, Biochemical Methods: Biological and other aspects.Category: esters-buliding-blocks

Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics

On April 5, 2016, Killinger, Bryan A.; Moszczynska, Anna published an article.Safety of Bis(2,5-dioxopyrrolidin-1-yl) glutarate The title of the article was Characterization of α-Synuclein Multimer Stoichiometry in Complex Biological Samples by Electrophoresis. And the article contained the following:

The aberrant aggregation of α-synuclein in the brain is a hallmark of Parkinson’s disease (PD). In vivo soluble α-synuclein occurs as a monomer and several multimers, the latter of which may be important for the biol. function of α-synuclein. Currently, there is a lack of reproducible methods to compare α-synuclein multimer abundance between complex biol. samples. Here we developed a method, termed “multimer-PAGE,” that combines in-gel chem. crosslinking with several common electrophoretic techniques to measure the stoichiometry of soluble α-synuclein multimers in brain tissue lysates. Results show that soluble α-synuclein from the rat brain exists as several high mol. weight species of approx. 56 kDa (αS56), 80 kDa (αS80), and 100 kDa (αS100) that comigrate with endogenous lipids, detergents, and/or micelles during blue native gel electrophoresis (BN-PAGE). Co-extraction of endogenous lipids with α-synuclein was essential for the detection of soluble α-synuclein multimers. Homogenization of brain tissue in small buffer volumes (>50 mg tissue per 1 mL buffer) increased relative lipid extraction and subsequently resulted in abundant soluble multimer detection via multimer-PAGE. α-Synuclein multimers captured by directly crosslinking soluble lysates resembled those observed following multimer-PAGE. The ratio of multimer (αS80) to monomer (αS17) increased linearly with protein input into multimer-PAGE, suggesting to some extent, multimers were also formed during electrophoresis. Overall, soluble α-synuclein maintains lipid interactions following tissue disruption and readily forms multimers when this lipid-protein complex is preserved. Once the multimer-PAGE technique was validated, relative stoichiometric comparisons could be conducted simultaneously between 14 biol. samples. Multimer-PAGE provides a simple inexpensive biochem. technique to study the mol. factors influencing α-synuclein multimerization. The experimental process involved the reaction of Bis(2,5-dioxopyrrolidin-1-yl) glutarate(cas: 79642-50-5).Safety of Bis(2,5-dioxopyrrolidin-1-yl) glutarate

The Article related to synuclein multimer assembly stoichiometry determination electrophoresis, Biochemical Methods: Electrical and other aspects.Safety of Bis(2,5-dioxopyrrolidin-1-yl) glutarate

Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics

On February 21, 2020, Geng, Xueyun; Wang, Guirong; Guo, Zhongwu; Gu, Guofeng published an article.Recommanded Product: Bis(2,5-dioxopyrrolidin-1-yl) glutarate The title of the article was Synthesis of the Oligosaccharides of Burkholderia pseudomallei and B. mallei Capsular Polysaccharide and Preliminary Immunological Studies of Their Protein Conjugates. And the article contained the following:

Efficient strategies were developed for the synthesis of 6-deoxy-D-manno-heptopyranose and its β-(1 â†?3)-linked oligomers as fragments of the common and major capsular polysaccharide, type I O-PS, of Burkholderia pseudomallei and Burkholderia mallei. The unusual heptose was synthesized from mannose, highlighted by the facile Wittig reaction and anti-Markovnikov hydroboration of the resultant olefin. The difficult β-mannosidic linkage in the oligosaccharides was achieved in high stereoselectivity by H-bond-mediated aglycon delivery. All of the oligosaccharides were conjugated with the carrier protein CRM197. Preliminary immunol. evaluations of the resultant glycoconjugates in mice verified their efficacy to elicit high titers of IgG antibodies and robust T-cell-dependent immune responses. It was also found that the trisaccharide conjugates provoked the strongest immune responses, worthy of further in-depth study for vaccine development. The experimental process involved the reaction of Bis(2,5-dioxopyrrolidin-1-yl) glutarate(cas: 79642-50-5).Recommanded Product: Bis(2,5-dioxopyrrolidin-1-yl) glutarate

The Article related to igg igm antibody oligosaccharide preparation vaccine glycosylation immunol protein, Carbohydrates: Oligosaccharides and other aspects.Recommanded Product: Bis(2,5-dioxopyrrolidin-1-yl) glutarate

Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics