Category: 6217-68-1

Baum, H. published the artcileSulfatases. XXI. The anomalous kinetics of arylsulfatase A of human tissues: the anomalies, Synthetic Route of 6217-68-1, the publication is Biochemical Journal (1958), 567-72, database is CAplus and MEDLINE.

cf. C.A. 52, 14725h. Preparations of human arylsulfatase A exhibit anomalous time-activity curves when incubated with di-K 2-hydroxy-5-nitrophenylsulfate (I), K p-acetylphenylsulfate, and K p-nitrophenyl sulfate. The rate of the reaction increases with time, at a rate which varies with the enzyme concentration The rate is proportional to some value greater than the 1st power of the enzyme concentration, depending upon the length of incubation time. Increasing substrate concentration during the initial period of incubation results in an increase in the rate to a maximum, whereas with prolonged incubation with the substrate the rate passes through a maximum and falls at higher substrate concentrations During the early incubation period there are optima at pH 4.4 and 5.0, whereas as the period of incubation is extended the 2 peaks merge at pH 4.4, and then shift to 4.7. The time-activity curves obtained at 20.5 and 30.5° cross those obtained at 37.5 and 50.5°; this indicates that enzyme destruction is not an important factor. The anomalous kinetics appear to be a feature peculiar to mammalian arylsulfatase A and are not shown by human arylsulfatase B or the arylsulfatases of limpets (Patella vulgata), Alcaligenes metalcaligenes, Taka-Diastase, and Helix pomatia.

Biochemical Journal published new progress about 6217-68-1. 6217-68-1 belongs to esters-buliding-blocks, auxiliary class Salt,Nitro Compound,Sulfonate,Benzene, name is Potassium 4-nitrophenyl sulfate, and the molecular formula is C6H4KNO6S, Synthetic Route of 6217-68-1.

Referemce:
https://en.wikipedia.org/wiki/Ester,
Ester – an overview | ScienceDirect Topics

Gautom, Trishnamoni published the artcileStructural basis of terephthalate recognition by solute binding protein TphC, Name: Potassium 4-nitrophenyl sulfate, the publication is Nature Communications (2021), 12(1), 6244, database is CAplus and MEDLINE.

Biol. degradation of Polyethylene terephthalate (PET) plastic and assimilation of the corresponding monomers ethylene glycol and terephthalate (TPA) into central metabolism offers an attractive route for bio-based mol. recycling and bioremediation applications. A key step is the cellular uptake of the non-permeable TPA into bacterial cells which has been shown to be dependent upon the presence of the key tphC gene. However, little is known from a biochem. and structural perspective about the encoded solute binding protein, TphC. Here, we report the biochem. and structural characterization of TphC in both open and TPA-bound closed conformations. This anal. demonstrates the narrow ligand specificity of TphC towards aromatic para-substituted dicarboxylates, such as TPA and closely related analogs. Further phylogenetic and genomic context anal. of the tph genes reveals homologous operons as a genetic resource for future biotechnol. and metabolic engineering efforts towards circular plastic bio-economy solutions

Nature Communications published new progress about 6217-68-1. 6217-68-1 belongs to esters-buliding-blocks, auxiliary class Salt,Nitro Compound,Sulfonate,Benzene, name is Potassium 4-nitrophenyl sulfate, and the molecular formula is C6H4KNO6S, Name: Potassium 4-nitrophenyl sulfate.

Referemce:
https://en.wikipedia.org/wiki/Ester,
Ester – an overview | ScienceDirect Topics

Fitzgerald, J. W. published the artcileThe occurrence of an inducible arylsulfatase in Comamonas terrigena, Name: Potassium 4-nitrophenyl sulfate, the publication is FEMS Microbiology Letters (1977), 2(4), 221-4, database is CAplus.

When C. terrigena was cultured in a synthetic mineral medium containing p-nitrophenyl sulfate (NPS) as S source, growth occurred after a 35-h lag and was accompanied by the increased ability of cell extracts to hydrolyze NPS and by the release from intact cells of p-nitrophenol. A S-mediated derepression mechanism for the pos. control of arylsulfatase is apparently absent in C. terrigena since the cells exhibited low activity after growth without added S or in the presence of Na₂SO₄ or methionine. NPS produced ∼2000-fold increase in arylsulfatase activity, indicating that the enzyme is substrate-inducible. NPS was the most effective inducer and substrate for arylsulfatase compared with p-nitrocatechol sulfate, p-acetylphenyl sulfate, and L-tyrosine-O-sulfate.

FEMS Microbiology Letters published new progress about 6217-68-1. 6217-68-1 belongs to esters-buliding-blocks, auxiliary class Salt,Nitro Compound,Sulfonate,Benzene, name is Potassium 4-nitrophenyl sulfate, and the molecular formula is C6H4KNO6S, Name: Potassium 4-nitrophenyl sulfate.

Referemce:
https://en.wikipedia.org/wiki/Ester,
Ester – an overview | ScienceDirect Topics

Rai, Kamal published the artcileEnhanced anticoagulant activity of hirudin-i analogue co-expressed with arylsulfotransferase in periplasm of E. coli BL21(DE3), Quality Control of 6217-68-1, the publication is Journal of Biotechnology (2020), 107-112, database is CAplus and MEDLINE.

Hirudin, a blood anticoagulant, is the most potent natural thrombin inhibitor of leech origin. Its application is limited because it is difficult to obtain abundant natural hirudin directly from the leech. Although some bioengineering methods can significantly increase the production of hirudin, the reduced efficacy of recombinant hirudin (rH) remains a critical shortcoming. The lack of sulfation of tyrosine 63 in rH is an important cause of its inadequate performance. This article is the first report of periplasmic co-expression of an rH-I analog with arylsulfotransferase (ASST) in E. coli BL21(DE3). Co-expressed rH-I analog with sulfate donor substrate (p-nitrophenyl sulfate potassium) showed anticoagulant (rabbit and goat serum) activity twice more than rH-I analog expressed without ASST, indicating its potential periplasmic sulfation. Moreover, purified rH-I analog showed above 4.5 times higher anticoagulant activity compared to therapeutic anti-thrombotic heparin (HE). At the same time, pH-dependent differential solubility was employed to purify rH analogs from fermentation broth, which is a simple, fast and inexpensive purification technol., and can potentially be used for larger scale purification This will also greatly improve the application of rH in clin. treatment.

Journal of Biotechnology published new progress about 6217-68-1. 6217-68-1 belongs to esters-buliding-blocks, auxiliary class Salt,Nitro Compound,Sulfonate,Benzene, name is Potassium 4-nitrophenyl sulfate, and the molecular formula is C6H4KNO6S, Quality Control of 6217-68-1.

Referemce:
https://en.wikipedia.org/wiki/Ester,
Ester – an overview | ScienceDirect Topics

Burehardt, George Norman published the artcileArylsulfuric acids, Recommanded Product: Potassium 4-nitrophenyl sulfate, the publication is Journal of the Chemical Society (1926), 684-90, database is CAplus.

CISO₂H (34 g.) run into a mixture of 100 g. PhNEt₂ (or PhNMe₂) in 100 cc. CS₂ at not over 10° and then treated with 25 g. PhOH in 50 cc. CS₂ gives, after treatment with a mixture of NaOH and Ba(OH)₂, 90% of NaPhSO₄, the PhNEt₂ being readily recovered. If the base and PhOH are mixed before the ClSO₂H is added the yield never exceeds 60%. If 19 g. SO₃ in 80 cc. liquid SO₂ is added to 20 g. PhOH and 60 g. PhNEt₂ with cooling, there results 60% of NaPhSO₄. p-O₂NC₆H₄OH (30 g.) added to 25 g. SO₂ and 29 g. C₅H₅N in 200 cc. boiling C₆H₆ gives 90-4% K p-nitrophenyl sulfate. Eugenol gives 70% of the sulfate. o-O₂NC₆H₄OH reacts less readily and gives 25% of the sulfate with an equivalent amount of reagent, 56% with twice the theory. PhOH and PhNEt₂ give 80-5% KPhSO₄ with 1.5 mols. K₂S₂O₇, 90% with 2 mols. The alkali salts of arylsulfuric acids are stable at 100° in neutral aqueous solution and require heating to boiling before rapid hydrolysis sets in in the presence of mineral acids. The Ba salt is decomposed by heating to 100° in H₂O for 1.5 hrs., after which hydrolysis proceeds rapidly to completion in 2 hrs. The NH₄ and Mg salts are of intermediate stability. All the salts are stable at 100° in aqueous AcONa, even in the presence of a considerable amount of AcOH. Hydrolysis of the K salt is also brought about by heating at 150° with strong alkali or with half concentrated NH₄OH at 190-220° for 4 hrs. K phenyl sulfate, rhombohedral or 6-sided plates with apex angle 95°, solubility in EtOH at 17° 0.7%, in H₂O 14%. Na salt, rhombic plates with 3H₂O, solubility in EtOH at 17°, 7%, in H₂O 31%. Ba salt, fine granules, solubility in EtOH 15%. The solubility of K o-tolyl sulfate in H₂O at 17° is 22%, of the m-derivative, 9%; of the p-derivative, 10%. p-Toluidine tolyl sulfates are characteristic. The o-cresol salt, m. 125-7° (decomposition), the p-cresol salt, 149-51°. Oxidation of K m-tolyl sulfate with KMnO₄ gives 80% of K m-carboxyphenyl sulfate, plates, 3.2 parts dissolving in 100 parts H₂O at 17°. K α-naphthyl sulfate, long needles, soluble in 40 parts. H₂O at 17°; the β-salt crystallines with 4H₂O and dissolves in 70 parts H₂O at 17°. Their oxidation was studied but definite products were not isolated.

Journal of the Chemical Society published new progress about 6217-68-1. 6217-68-1 belongs to esters-buliding-blocks, auxiliary class Salt,Nitro Compound,Sulfonate,Benzene, name is Potassium 4-nitrophenyl sulfate, and the molecular formula is C6H4KNO6S, Recommanded Product: Potassium 4-nitrophenyl sulfate.

Referemce:
https://en.wikipedia.org/wiki/Ester,
Ester – an overview | ScienceDirect Topics

Conway, Walter D. published the artcileSolvent selection for countercurrent chromatography by rapid estimation of partition coefficients and application to polar conjugates of p-nitrophenol, Computed Properties of 6217-68-1, the publication is Journal of Liquid Chromatography (1984), 7(2), 275-89, database is CAplus.

A rapid and moderately precise technique to measure partition coefficients of UV-absorbing solutes in solvent systems for countercurrent chromatog. is described and applied to p-nitrophenol and its conjugates with glucose, H₂SO₄ and glucuronic acid. It involves equilibration of 1 mL of each phase with solute in a narrow test tube, removal of the entire lower phase, dilution with MeOH and calculation of the partition coefficient as the ratio of the absorbance values of each dilution at any suitable wavelength. The polar conjugates of p-nitrophenol can be separated by countercurrent chromatog. by using EtOAc as mobile phase and aqueous KH₂PO₄ as stationary phase.

Journal of Liquid Chromatography published new progress about 6217-68-1. 6217-68-1 belongs to esters-buliding-blocks, auxiliary class Salt,Nitro Compound,Sulfonate,Benzene, name is Potassium 4-nitrophenyl sulfate, and the molecular formula is C6H4KNO6S, Computed Properties of 6217-68-1.

Referemce:
https://en.wikipedia.org/wiki/Ester,
Ester – an overview | ScienceDirect Topics

Gemborys, Mark W. published the artcileSynthesis of N-hydroxyacetaminophen, a postulated toxic metabolite of acetaminophen, and its phenolic sulfate conjugate, Quality Control of 6217-68-1, the publication is Journal of Medicinal Chemistry (1978), 21(7), 649-52, database is CAplus and MEDLINE.

The synthesis of N-hydroxyacetaminophen (I) [63975-21-3], a postulated toxic metabolite of acetaminophen, and its phenolic sulfate conjugate II [66682-85-7] is described. K p-nitrophenyl sulfate [6217-68-1] was reduced, acetylated, and treated with sulfatase to give I. I was moderately unstable at physiol. pH and temperature, but II was stable.

Journal of Medicinal Chemistry published new progress about 6217-68-1. 6217-68-1 belongs to esters-buliding-blocks, auxiliary class Salt,Nitro Compound,Sulfonate,Benzene, name is Potassium 4-nitrophenyl sulfate, and the molecular formula is C6H4KNO6S, Quality Control of 6217-68-1.

Referemce:
https://en.wikipedia.org/wiki/Ester,
Ester – an overview | ScienceDirect Topics

Kreutz, Jennifer Anna published the artcileA 1-year study of the activities of seven hydrolases in a communal wastewater treatment plant: trends and correlations, Related Products of esters-buliding-blocks, the publication is Applied Microbiology and Biotechnology (2016), 100(15), 6903-6915, database is CAplus and MEDLINE.

The activities of seven hydrolytic enzymes (L-alanine aminopeptidase, esterase, α-and β-glucosidase, phosphomonoesterase, phosphodiesterase, sulfatase) were monitored during 1 yr in parallel and serial treatment units of the biol. stage of a communal wastewater treatment plant. The spatial homogeneity of enzyme activities was high (coefficients of variation <10% for the entire treatment stage). A significant difference between aerated and stirred tanks was not observed Temperature seemed not to exert a direct influence. Long periods with comparably constant activities were interrupted by a few strong, short-time rises. The mean enzyme activities followed the sequence sulfatase < α-glucosidase < phosphodiesterase ≈ β-glucosidase≈esterase < phosphomonoesterase < L-alanine aminopeptidase. The enzyme activities correlated among themselves at different levels. Very strong (r > 0.8) and highly significant (p < 0.01) correlations between the activities of both glucosidases, both phosphoesterases, and between phosphomonoesterase and both glucosidases were ascertained, pointing to the importance of substrate specificity and similarity of metabolic functions. Moderate and strong activity correlations with various wastewater constituents and with process parameters, e.g., concentrations, loads and eliminated amounts of phosphorous, TOC concentrations and loads of the plant effluent, dry matter content of activated sludge, and sludge volume, were found.

Applied Microbiology and Biotechnology published new progress about 6217-68-1. 6217-68-1 belongs to esters-buliding-blocks, auxiliary class Salt,Nitro Compound,Sulfonate,Benzene, name is Potassium 4-nitrophenyl sulfate, and the molecular formula is C6H4KNO6S, Related Products of esters-buliding-blocks.

Referemce:
https://en.wikipedia.org/wiki/Ester,
Ester – an overview | ScienceDirect Topics

Murray, Stephen published the artcileDirect derivatization of sulfate esters for analysis by gas chromatography mass spectrometry, Synthetic Route of 6217-68-1, the publication is Biomedical Mass Spectrometry (1979), 6(2), 82-9, database is CAplus.

Gas chromatog.-mass spectrometry was used to study the reaction between 13 aromatic, steroidal and aliphatic sulfate esters and 3 perfluoroacylating reagents, viz. (CF₃CO)₂O, (C₂F₅CO)₂O and (CF₃CF₂CF₂CO)₂O. Aromatic sulfates reacted readily with 1:1 anhydride:AcOEt to give the perfluoro ester of the parent phenol quant. Nonaromatic sulfates gave a variety of products. A mechanism is proposed for the 1-step derivatization of aromatic sulfates, and the potential of the procedure for the anal. of sulfate conjugates by mass spectrometry is discussed.

Biomedical Mass Spectrometry published new progress about 6217-68-1. 6217-68-1 belongs to esters-buliding-blocks, auxiliary class Salt,Nitro Compound,Sulfonate,Benzene, name is Potassium 4-nitrophenyl sulfate, and the molecular formula is C6H4KNO6S, Synthetic Route of 6217-68-1.

Referemce:
https://en.wikipedia.org/wiki/Ester,
Ester – an overview | ScienceDirect Topics