Velema, Willem A. published the artcileTrapping Transient RNA Complexes by Chemically Reversible Acylation, Synthetic Route of 51984-71-5, the main research area is Escherichia DsrA rpoS RNA complex acylation BINARI crosslinker; RNA complexes; acylation; bioorthogonal chemistry; crosslinking; nucleic acids.
RNA-RNA interactions are essential for biol., but they can be difficult to study due to their transient nature. While crosslinking strategies can in principle be used to trap such interactions, virtually all existing strategies for crosslinking are poorly reversible, chem. modifying the RNA and hindering mol. anal. We describe a soluble crosslinker design (BINARI) that reacts with RNA through acylation. We show that it efficiently crosslinks noncovalent RNA complexes with mimimal sequence bias and establish that the crosslink can be reversed by phosphine reduction of azide trigger groups, thereby liberating the individual RNA components for further anal. The utility of the new approach is demonstrated by reversible protection against nuclease degradation and trapping transient RNA complexes of E. coli DsrA-rpoS derived bulge-loop interactions, which underlines the potential of BINARI crosslinkers to probe RNA regulatory networks.
Angewandte Chemie, International Edition published new progress about Acylation. 51984-71-5 belongs to class esters-buliding-blocks, name is Ethyl 2-methyl-5-nitro-3-pyridinecarboxylate, and the molecular formula is C9H10N2O4, Synthetic Route of 51984-71-5.
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