Ren, Sumei et al. published their research in Journal of Labelled Compounds and Radiopharmaceuticals in 2009 |CAS: 220851-46-7

The Article related to monoclonal antibody 35s radiolabeled preparation, radiolabel smsb msappa preparation acylation reductive alkylation lysine protein, Amino Acids, Peptides, and Proteins: Protein Synthesis and other aspects.Category: esters-buliding-blocks

On June 30, 2009, Ren, Sumei; McNamara, Paul; Koharski, David; Hesk, David; Borges, Scott published an article.Category: esters-buliding-blocks The title of the article was Design and synthesis of sulfur-35 agents and their applications for protein labeling. And the article contained the following:

Two new 35S reagents were developed to radiolabel proteins. The first reagent, SMSB (I), acylates the ε-amino group of lysine residues in proteins. The second reagent, MSAPPA (II), labels lysine residues via reductive alkylation. Comparing the two methods, the reductive alkylation method labeled proteins over a broader pH range with higher overall radiochem. yield. More than ten monoclonal antibodies (mAbs) have been labeled with these 35S labeling reagents, the biol. activity of the mAbs was unchanged. The experimental process involved the reaction of tert-Butyl 4-((((benzyloxy)carbonyl)amino)methyl)benzoate(cas: 220851-46-7).Category: esters-buliding-blocks

The Article related to monoclonal antibody 35s radiolabeled preparation, radiolabel smsb msappa preparation acylation reductive alkylation lysine protein, Amino Acids, Peptides, and Proteins: Protein Synthesis and other aspects.Category: esters-buliding-blocks

Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics

Kawata, Mitsuyasu et al. published their patent in 1999 |CAS: 220851-46-7

The Article related to oxamyl immunoassay antibody production hapten preparation, hybridoma monoclonal antibody oxamyl immunoassay, Agrochemical Bioregulators: Methods (Including Analysis) and other aspects.Category: esters-buliding-blocks

On February 9, 1999, Kawata, Mitsuyasu; Morimune, Kosuke; Takewaki, Shunichi; Miyake, Shirou; Yamaguchi, Yuki; Beppu, Yoshinori published a patent.Category: esters-buliding-blocks The title of the patent was Immunoassay of oxamyl, and haptens, their conjugates, and hybridomas for production of antibodies to oxamyl. And the patent contained the following:

Me2NCOC(SMe):NOCONH(CH2)lCO2H (I; l = 6-15), Me2NCOC(SMe):NOCONHR1CO2H [R1 = CH2A (A = cyclohexyl), cyclohexylene, CH2C6H4, C6H4, which may be substituted with 1-2 halo, C1-3 alkyl], Me2NCOC(SMe):NOR2CO2H [II; R2 = (CH2)m (m = 1-3), CH2CONH(CH2)n (n = 1-5), CO(CH2)o (o = 1-5)], or Me3CC(CH2SR3):NOCONH(CH2)pCO2H (R3 = C1-6 alkyl; p = 1-10) are useful as haptens to produce antibodies or their fragments specific to oxamyl (III). The antibodies, their fragments, and conjugates of the haptens with macromols. or labeling agents are useful for immunoassay of III. Hybridomas producing monoclonal antibodies are also claimed. Mouse was immunized with I (l = 5) (preparation given) conjugated with keyhole limpet hemocyanin by the conventional method. Splenic cells of the mouse was fused with myeloma cells to give hybridomas producing a monoclonal antibody OXM6-8. ELISA for III was performed using a microtiter plate coated with OXM6-8 and HRP-labeled II [R2 = CO(CH2)4]. The system was highly specific to III. The experimental process involved the reaction of tert-Butyl 4-((((benzyloxy)carbonyl)amino)methyl)benzoate(cas: 220851-46-7).Category: esters-buliding-blocks

The Article related to oxamyl immunoassay antibody production hapten preparation, hybridoma monoclonal antibody oxamyl immunoassay, Agrochemical Bioregulators: Methods (Including Analysis) and other aspects.Category: esters-buliding-blocks

Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics

Ren, Sumei et al. published their research in Journal of Labelled Compounds and Radiopharmaceuticals in 2007 |CAS: 220851-46-7

The Article related to protein labeling sulfur 35 reagent benzoate phenylpropanal preparation, Benzene, Its Derivatives, and Condensed Benzenoid Compounds: Esters, Acyl Peroxides, Acyl Halides and other aspects.Computed Properties of 220851-46-7

On May 31, 2007, Ren, Sumei; Mcnamara, Paul; Koharski, David; Hesk, David; Borges, Scott published an article.Computed Properties of 220851-46-7 The title of the article was Synthesis of sulfur-35 reagents for protein labeling. And the article contained the following:

Two 35S reagents were developed to radiolabel proteins. The first reagent, Me35SO2NHCH2C6H4CO2H-p N-hydroxysuccinimide (NHS) ester (SMSB), acylates the ε-amino group of lysine residues in proteins. The second reagent, aldehyde Me35SO2NHCH2C6H4CH2CH2CHO-p (MSAPPA), labels lysine residues via reductive alkylation. Comparing the two methods, the reductive alkylation method labeled proteins over a broader pH range with higher overall radiochem. yield. The biol. activity of the proteins did not change after labeling with these 35S reagents. The experimental process involved the reaction of tert-Butyl 4-((((benzyloxy)carbonyl)amino)methyl)benzoate(cas: 220851-46-7).Computed Properties of 220851-46-7

The Article related to protein labeling sulfur 35 reagent benzoate phenylpropanal preparation, Benzene, Its Derivatives, and Condensed Benzenoid Compounds: Esters, Acyl Peroxides, Acyl Halides and other aspects.Computed Properties of 220851-46-7

Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics

Pitts, William J. et al. published their patent in 1999 |CAS: 220851-46-7

The Article related to pyrimidinylalkylphenylcarbonylaminopropanoate preparation integrin antagonist, quinazolinylaminomethylphenylcarbonylaminopropanoate preparation integrin antagonist, cell adhesion inhibitor pyrimidine quinazoline triazine preparation and other aspects.Name: tert-Butyl 4-((((benzyloxy)carbonyl)amino)methyl)benzoate

On October 7, 1999, Pitts, William J.; Jadhav, Prabhakar K. published a patent.Name: tert-Butyl 4-((((benzyloxy)carbonyl)amino)methyl)benzoate The title of the patent was Preparation of pyrimidinylalkylphenylcarbonylaminopropanoates and related compounds as integrin antagonists. And the patent contained the following:

GT (T = integrin antagonist template; G = specified guanidine mimic), were prepared as antagonists of the αvβ3 integrin, the α2bβ3 integrin, and related cell surface adhesive protein receptors for the inhibition of cell adhesion, treatment of angiogenic disorders, inflammation, bone degradation, cancer metastasis, diabetic retinopathy, thrombosis, restenosis, macular degeneration, and other conditions mediated by cell adhesion and/or cell migration and/or angiogenesis. Thus, 2-[(S)-2,4,6-trimethylphenylsulfonylamino]-3-[4-[2-(2,4-diaminopyrimidin-6-yl)ethyl]phenylcarbonylamino]propionic acid trifluoroacetate was prepared in several steps from L-asparagine. In the αvβ3-vitronectin assay, tested title compounds showed IC50≤10 μM. The experimental process involved the reaction of tert-Butyl 4-((((benzyloxy)carbonyl)amino)methyl)benzoate(cas: 220851-46-7).Name: tert-Butyl 4-((((benzyloxy)carbonyl)amino)methyl)benzoate

The Article related to pyrimidinylalkylphenylcarbonylaminopropanoate preparation integrin antagonist, quinazolinylaminomethylphenylcarbonylaminopropanoate preparation integrin antagonist, cell adhesion inhibitor pyrimidine quinazoline triazine preparation and other aspects.Name: tert-Butyl 4-((((benzyloxy)carbonyl)amino)methyl)benzoate

Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics