Construction and analysis of a food-grade Lactiplantibacillus plantarum esterase/lipase overexpression system was written by He, Xi;Liu, Peng;Tang, Xiaojian;Wang, Ting;Xu, Zhenshang;Hou, Dongdong;Wu, Dan;Han, Ning. And the article was included in LWT–Food Science and Technology in 2022.Safety of Isopentyl hexanoate The following contents are mentioned in the article:
Lactiplantibacillus plantarum (L. plantarum) is widely used in the food industry, especially in meat fermentation and dairy processing, due to its lipase and esterase activities. In this study, lp_1002 from L. plantarum WCFS1 was cloned into the food-grade gene expression vector pMG36n, with nisin used as a selective marker. Furthermore, pMG36n- lp_1002 was retransformed into L. plantarum WCFS1, and lp_1002 was overexpressed in the newly constructed L. plantarum WCFS1 strain. Moreover, recombinant Lp_1002 showed the characteristics of both lipase and esterase. The results from hydrolysis of p-nitrophenylphosphate (pNPP) and triglycerides showed that the hydrolysis activities of the recombinant Lp_1002 were 420% and 360% higher than that of the original strain, resp. In addition, the results of wine MLF fermentation suggest that the recombinant strain significantly reduced the contents of Et esters, acetate esters and other types of esters, which affected wine aroma during brewing. Therefore, the recombinant strain has great application potential in food fermentation This study involved multiple reactions and reactants, such as Isopentyl hexanoate (cas: 2198-61-0Safety of Isopentyl hexanoate).
Isopentyl hexanoate (cas: 2198-61-0) belongs to esters. Esters typically have a pleasant smell; those of low molecular weight are commonly used as fragrances and are found in essential oils and pheromones. Liquid esters of low volatility serve as softening agents for resins and plastics. Esters also include many industrially important polymers. Polymethyl methacrylate is a glass substitute sold under the names Lucite and Plexiglas; polyethylene terephthalate is used as a film (Mylar) and as textile fibres sold as Terylene, Fortrel, and Dacron.Safety of Isopentyl hexanoate
Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics