Liu, Yiliu; Turunen, Petri; de Waal, Bas F. M.; Blank, Kerstin G.; Rowan, Alan E.; Palmans, Anja R. A.; Meijer, E. W. published the artcile< Catalytic single-chain polymeric nanoparticles at work: from ensemble towards single-particle kinetics>, Computed Properties of 71195-85-2, the main research area is catalytic single chain polymeric nanoparticle design preparation.
Folding a single polymer chain around catalytically active sites to construct catalytic single chain polymeric nanoparticles (SCPNs) is a novel approach to mimic the activity and selectivity of enzymes. In order to relate the efficiency of SCPNs to their three-dimensional structure, a better understanding of their catalytic activity at an individual level, rather than at an ensemble level, is highly desirable. In this work, we present the design and preparation of catalytic SCPNs and a family of fluorogenic substrates, their characterization at the ensemble level as well as our progress toward analyzing individual SCPNs with single-mol. fluorescence microscopy. Firstly, organocopper-based SCPNs together with rhodamine-based fluorogenic substrates were designed and synthesized. The SCPNs catalyze the carbamate cleavage reaction of mono-protected rhodamines, with the dimethylpropargyloxycarbonyl protecting group being cleaved most efficiently. A systematic study focusing on the conditions during catalysis revealed that the ligand acceleration effect as well as the accumulation of substrates and catalytically active sites in SCPNs significantly promote their catalytic performance. Secondly, a streptavidin-biotin based strategy was developed to immobilize the catalytic SCPNs on the surface of glass coverslips. Fluorescence correlation spectroscopy experiments confirmed that the SCPNs remained catalytically active after surface immobilization. Finally, single-SCPN activity measurements were performed. The results qual. indicated that fluorescent product mols. were formed as a result of the catalytic reaction and that individual fluorescent product mols. could be detected. So far, no evidence for strongly different behaviors has been observed when comparing individual SCPNs.
Molecular Systems Design & Engineering published new progress about Catalysts. 71195-85-2 belongs to class esters-buliding-blocks, and the molecular formula is C9H3F5O2, Computed Properties of 71195-85-2.
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