Pyser, Joshua B.;Baker Dockrey, Summer A.;Benitez, Attabey Rodriguez;Joyce, Leo A.;Wiscons, Ren A.;Smith, Janet L.;Narayan, Alison R. H. published 《Stereodivergent, Chemoenzymatic Synthesis of Azaphilone Natural Products》 in 2019. The article was appeared in 《Journal of the American Chemical Society》. They have made some progress in their research.Reference of Methyl 2,4-dihydroxy-3,6-dimethylbenzoate The article mentions the following:
Selective access to a targeted isomer is often critical in the synthesis of biol. active mols. Whereas small-mol. reagents and catalysts often act with anticipated site- and stereoselectivity, this predictability does not extend to enzymes. Further, the lack of access to catalysts that provide complementary selectivity creates a challenge in the application of biocatalysis in synthesis. Here, we report an approach for accessing biocatalysts with complementary selectivity that is orthogonal to protein engineering. Through the use of a sequence similarity network (SSN), a number of sequences were selected, and the corresponding biocatalysts were evaluated for reactivity and selectivity. With a number of biocatalysts identified that operate with complementary site- and stereoselectivity, these catalysts were employed in the stereodivergent, chemoenzymic synthesis of azaphilone natural products. Specifically, the first syntheses of trichoflectin, deflectin-1a, and lunatoic acid A were achieved. In addition, chemoenzymic syntheses of these azaphilones supplied enantioenriched material for reassignment of the absolute configuration of trichoflectin and deflectin-1a based on optical rotation, CD spectra, and X-ray crystallog. To complete the study, the researchers used Methyl 2,4-dihydroxy-3,6-dimethylbenzoate (cas: 4707-47-5) .
Methyl 2,4-dihydroxy-3,6-dimethylbenzoate(4707-47-5) is used in biological study as role of androgens in inducing distinct response of epithelial-mesenchymal transition factors in human prostate cancer cells.Reference of Methyl 2,4-dihydroxy-3,6-dimethylbenzoate
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