Formula: C19H36O2In 2022, Maidana Serpa, John Dayvan;Cavalieri de Alencar Guimaraes, Nelciele;Kioshi Yonekawa, Murilo Aquino;Pereira de Almeida, Aline;Ruller, Roberto;Augusto dos Santos Jaques, Jeandre;Santos, Edson dos Anjos dos;Masui, Douglas Chodi;Zanoelo, Fabiana Fonseca;Giannesi, Giovana Cristina published 《Sarocladium strictum lipase (LipSs) produced using crude glycerol as sole carbon source: A promising enzyme for biodiesel production》. 《Biocatalysis and Agricultural Biotechnology》published the findings. The article contains the following contents:
The production of biodiesel (fatty acid Me esters), an environmentally safe next-generation biofuel, using a lipase from Sarocladium strictum and glycerol (a biodiesel byproduct) as sole carbon source, was investigated. The fungus S. strictum showed maximum lipase production when grown in crude glycerol (3.13 U/mg protein) with 168 h of growth. Different concentrations of crude glycerol as carbon source were tested and concentrations ranging from 0.5 to 4% were completely consumed (100%) by the S. strictum. The lipase from S. strictum was purified (24.4-fold) with a specific activity of 73.2 U/mg protein showing a mol. weight of 52 kDa. The effects of pH and temperature in the lipase activity were evaluated showing optimal activity at pH 5.0 and 40°C, resp. Lipase hydrolyzed different substrates and exhibited better activity on p-nitrophenyl octanoate (30.68 U/mg protein). The detergents Triton X-100 and Tween 80 at concentration of 0.1% (volume/volume) increased the lipase activity by 10.45 and 8.55%, resp. The lipase maintained 80% residual activity in the presence of methanol (1:1, volume/volume) after 24 h of incubation. In concentrations ranging from 1 to 5% of methanol, showed activity above 90%, maintaining 70% activity at 10% concentration Gas chromatog. mass spectrometry (GC-MS) anal. confirmed biodiesel production with soybean and sunflower oil by lipase with a conversion rate of approx. 65 and 85%, resp. Therefore, the lipase was efficient in the conversion of low-cost plant oil to high-value biodiesel.Methyl oleate (cas: 112-62-9) were involved in the experimental procedure.
Methyl oleate(cas: 112-62-9) is an intermediate for detergents, emulsifiers, wetting agents, stabilizers, textile treatments, plasticizers for duplicating inks, rubbers, waxes etc.Formula: C19H36O2 It is also used in biochemical research as a chromatographic reference standard.
Reference:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics