Njateng, Guy Sedar Singor;Du, Zhizhi;Gatsing, Donatien;Donfack, Arno Rusel Nanfack;Talla, Michel Feussi;Wabo, Hippolyte Kamdem;Tane, Pierre;Mouokeu, Raymond Simplice;Luo, Xiaodong;Kuiate, Jules-Roger published 《Antifungal properties of a new terpernoid saponin and other compounds from the stem bark of Polyscias fulva Hiern (Araliaceae)》. The research results were published in《BMC Complementary and Alternative Medicine》 in 2015.Safety of Methyl 2,4-dihydroxy-3,6-dimethylbenzoate The article conveys some information:
Background: In our previous studies, it was evident that the dichloromethane-methanol (1:1 volume/volume) stem barks extract of Polyscias fulva and fractions (Et acetate, n-butanol and residue) demonstrated interesting antidermatophytic activities. So, as a continuity of that, this work aimed at identifying active principles with antifungal properties from P. fulva that could be used as markers for possible standardization of this plant as phytomedicine. Methods: The Et acetate, n-butanol and residual fractions of the dichloromethane-methanol (1:1 volume/volume) stem bark extract of Polyscias fulva were further fractionated by column chromatog. and the structures of isolated compounds elucidated based on their spectroscopic data in comparison with existing literature information. Antifungal activity was assayed by broth microdilution techniques on yeasts and dermatophytes spores. Results: The fractionation of the crude dichloromethane-methanol (1:1 volume/volume) stem bark extract of Polyscias fulva led to the isolation of 10 known compounds (1 to 10) and one new saponin (11: 3-O-[α-L-rhamnopyranosyl (1-2)-α-L-arabinopyranosyl]-28-O-[α-L-4-O-acetyl-rhamnopyranosyl (1-4)-β-D-glucopyranosyl-(1-6)-β-D-glucopyranosyl]-hederagenin). Among these compounds, 3-O-α-L- arabinopyranosyl-hederagenin and 3-O-[α-L-rhamnopyranosyl (1-2)-α-L-arabinopyranosyl]-hederagenin were the most active on the tested fungi with MIC values ranging from 0.78 to 100 μg/mL against both yeasts and dermatophytes. Conclusion: The results of this work constitute a step forward in the possible development of an antidermatophytic phytomedicine from Polyscias fulva stem bark, the isolated compounds being possible markers for the standardisation. And Methyl 2,4-dihydroxy-3,6-dimethylbenzoate (cas: 4707-47-5) was used in the research process.
Methyl 2,4-dihydroxy-3,6-dimethylbenzoate(4707-47-5) is used in biological study as role of androgens in inducing distinct response of epithelial-mesenchymal transition factors in human prostate cancer cells.Safety of Methyl 2,4-dihydroxy-3,6-dimethylbenzoate
Reference:
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