Development of ELISAs for the class-specific determination of organophosphorus pesticides was written by Piao, Yuan Zhe;Kim, Yoo Jung;Kim, Young Ah;Lee, Hye-Sung;Hammock, Bruce D.;Lee, Yong Tae. And the article was included in Journal of Agricultural and Food Chemistry in 2009.Application of 28478-46-8 This article mentions the following:
Enzyme-linked immunosorbent assays (ELISAs) for the class-specific determination of organophosphorus (OP) pesticides were developed from monoclonal antibodies raised against haptens with the functional group common to OP pesticides. To develop antigen-coated, indirect, competitive ELISAs, four haptens with different spacer arm structures were used to prepare antibodies, while eight haptens were tested for use as coating antigens. A total of 32 ELISAs were developed with one selected as the most suitable one based on average IC50 and % CV values. The chosen ELISA showed class-selective response to O,O-di-Et phosphorothioate and phosphorodithioate OP pesticides with negligible cross-reactivity to other types of pesticides. Average IC50 and % CV values of this ELISA for the 12 OP pesticides were 89 ng/mL and 96%, resp. Compared to ELISAs previously developed with the same objective, the current ELISA demonstrates better sensitivity based on much lower mean IC50 values in addition to improved class-selective determination based on considerably lower % CV values as well as precise discrimination against other types of pesticides. In the experiment, the researchers used many compounds, for example, Methyl 4-Hydroxy-2-methoxybenzoate (cas: 28478-46-8Application of 28478-46-8).
Methyl 4-Hydroxy-2-methoxybenzoate (cas: 28478-46-8) belongs to esters. Volatile esters with characteristic odours are used in synthetic flavours, perfumes, and cosmetics. Certain volatile esters are used as solvents for lacquers, paints, and varnishes. Esters contain a carbonyl center, which gives rise to 120掳 C鈥揅鈥揙 and O鈥揅鈥揙 angles. Unlike amides, esters are structurally flexible functional groups because rotation about the C鈥揙鈥揅 bonds has a low barrier. Their flexibility and low polarity is manifested in their physical properties; they tend to be less rigid (lower melting point) and more volatile (lower boiling point) than the corresponding amides. Application of 28478-46-8
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Ester – an overview | ScienceDirect Topics