Wang, Shubin published the artcileGlucose-coated berberine nanodrug for glioma therapy through mitochondrial pathway, Quality Control of 2044-85-1, the main research area is glucose berberine glioma cell mitochondria nanocarrier; berberine; glioma; glucose-nanocarrier; mitochondria; nanoparticles.
Introduction: Glioma is the primary malignant brain tumor with poor prognosis. Berberine (BBR) was the potential drug for anti-tumor in glioma cells. Based on its limitation of poor aqueous solubility and instability, little information of BBR nanoparticles is reported in glioma. Methods: Different solutions including 5% glucose, 1*PBS, ddH2O, 0.9% NaCl, cell culture medium were selected, and only 5% glucose and ddH2O exhibited BBR-related nanoparticles. After heating for a longer time or adding a higher concentration of glucose solution, BBR nanoparticles were detected by TEM anal. The uptake of BBR-Glu or BBR-Water nanoparticles were detected by immunofluorescence anal. for BBR autofluorescence. Cell viability was measured by MTT assay and Western blotting anal. Apoptosis was performed with flow cytometric anal. and was detected by cleaved caspase-3 immuno-fluorescent staining. Cell cycle was used by flow cytometric anal. Cytoskeleton was observed by confocal anal. using the neuron specific Class III β-tubulin and β-tubulin antibodies. Mitochondrial-related proteins were detected by Western blotting analyses and mito-tracker staining in live cells. Mitochondrion structures were observed by TEM anal. ROS generation and ATP production were detected by related com. kits. The tracking of BBR-Glu or BBR-Water nanoparticles into blood-brain barrier was observed in primary tumor-bearing models. The fluorescence of BBR was detected by confocal analyses in brains and gliomas.
International Journal of Nanomedicine published new progress about Apoptosis. 2044-85-1 belongs to class esters-buliding-blocks, name is 2′,7′-Dichloro-3-oxo-3H-spiro[isobenzofuran-1,9′-xanthene]-3′,6′-diyl diacetate, and the molecular formula is C24H14Cl2O7, Quality Control of 2044-85-1.
Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics