Hwang, Jee-hyun published the artcileEmployment of cytology for in vitro skin irritation test using a reconstructed human epidermis model, Keraskin, Application In Synthesis of 6259-76-3, the main research area is skin irritation epidermis irritant model Keraskin; Cytology; Dermal toxicity; Reconstructed human epidermis model; Skin irritation.
Skin irritation tests using reconstructed human epidermis (RhE) employ viability as an endpoint, but color interference or borderline results are often problematic. We examined whether the cytol. of cells from treated RhE could determine skin irritancy. Six chems. (three irritants; DnP, 1-B, PH, three non-irritants; DP, APA, HS) were evaluated in a RhE, Keraskin. DP, HS, and PH were clearly classified with viability, but DnP, 1-B, and APA were often falsely determined, due to borderline values falling near the cutoff, 50%. In histol., the tissues treated with DnP, 1-B, and PH showed erosion of the stratum corneum, vacuolization, and necrosis in the basal layer. DP- and HS-treated tissues showed relatively normal morphol. but APA induced necrosis similar to irritants. Cytol. revealed that DnP, 1-B or PH depleted cells and induced irregular and abnormal cell shapes. In contrast, relatively regular and normal shapes and clear distinction between the nucleus and cytoplasm was observed for DP, APA and HS. To further confirm it, addnl. 10 substances, including false positives from OECD TG 439, were tested. Overall (16 substances in total), cytol.: total area predicted the skin irritancy of test chems. with the highest accuracy (87.5%) followed by cytol.: cell count (81.3%), histol. (75%) and viability (68.8%), confirming the utility of cytol. as an alternative endpoint in the skin irritation test using RhE.
Toxicology In Vitro published new progress about Cytotoxicity. 6259-76-3 belongs to class esters-buliding-blocks, name is Hexyl 2-hydroxybenzoate, and the molecular formula is C13H18O3, Application In Synthesis of 6259-76-3.
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