Luganini, Anna published the artcileThe isoquinoline alkaloid berberine inhibits human cytomegalovirus replication by interfering with the viral Immediate Early-2 (IE2) protein transactivating activity., Application of 2-((5-Nitrothiazol-2-yl)carbamoyl)phenyl acetate, the main research area is HCMV virus replication IE2 isoquinoline alkaloid berberine; Berberine chloride; Human cytomegalovirus; IE2; IE3; Murine cytomegalovirus; Promoter transactivation.
The identification and validation of new small mols. able to inhibit the replication of HCMV remains a priority to develop alternatives to the currently used DNA polymerase inhibitors, which are often burdened by long-term toxicity and emergence of cross-resistance. Low micromolar concentrations of BBR were confirmed to suppress the replication of different HCMV strains, including clin. isolates and strains resistant to approved DNA polymerase inhibitors. Anal. of the HCMV replication cycle in infected cells treated with BBR then revealed that the bioactive compound compromised the progression of virus cycle at a stage prior to viral DNA replication and Early (E) genes expression, but after Immediate-Early (IE) proteins expression. Mechanistic studies in fact highlighted that BBR interferes with the transactivating functions of the viral IE2 protein, thus impairing efficient E gene expression and the progression of HCMV replication cycle. Finally, the mechanism of the antiviral activity of BBR appears to be conserved among different CMVs, since BBR suppressed MCMV replication and inhibited the transactivation of the prototypic MCMV E1 gene by the IE3 protein, the murine homolog of IE2. Together, these observations warrant for further experimentation to obtain proof of concept that BBR could represent an attractive candidate for alternative anti-HCMV therapeutic strategies.
Antiviral Research published new progress about Alkaloids Role: THU (Therapeutic Use), BIOL (Biological Study), USES (Uses). 55981-09-4 belongs to class esters-buliding-blocks, name is 2-((5-Nitrothiazol-2-yl)carbamoyl)phenyl acetate, and the molecular formula is C12H9N3O5S, Application of 2-((5-Nitrothiazol-2-yl)carbamoyl)phenyl acetate.
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