Phytochemical Investigations and In Vitro Bioactivity Screening on Melia azedarach L. Leaves Extract from Nepal was written by Shrestha, Shyam Sharan;Ferrarese, Irene;Sut, Stefania;Zengin, Gokhan;Grana, Sara;Ak, Gunes;Pant, Deepak Raj;Dall’Acqua, Stefano;Rajbhandary, Sangeeta. And the article was included in Chemistry & Biodiversity in 2021.Electric Literature of C8H16O2 The following contents are mentioned in the article:
Melia azedarach is a common tree used in the traditional medicine of Nepal. In this work, leaves were considered as source of bioactive constituents and composition of methanol extract was evaluated and compared with starting plant material. Flavonoid glycosides and limonoids were identified and quantified by HPLC-DAD-MSn approaches in dried leaves and methanolic extract, while HPLC-APCI-MSn and GC/MS anal. were used to study phytosterol and lipid compositions β-Sitosterol and rutin were the most abundant constituents. HPLC-APCI-MSn and HPLC-DAD-MSn anal. revealed high levels of phytosterols and flavonoids in methanolic extract accounting 9.6 and 7.5% on the dried weight, resp. On the other hand, HPLC/MSn data revealed that limonoid constituents were in minor amount in the extract <0.1%, compared with leaves (0.7%) indicating that degradation occurred during extraction or concentration procedures. The methanol extract was subjected to different bioassays, and antioxidant activity was evaluated. Limited inhibitory activity on acetyl and butyryl cholinesterase, as well as on amylase were detected. Moreover, tyrosinase inhibition was significant resulting in 131.57±0.51 mg kojic acid equivalent/g of dried methanol extract, suggesting possible use of this M. azedarach extract in skin hyperpigmentation conditions. Moderate cytotoxic activity, with IC50 of 26.4μg/mL was observed against human ovarian cancer cell lines (2008 cells). Our findings indicate that the Nepalese M. azedarach leaves can be considered as valuable starting material for the extraction of phenolics and phytosterols, yielding extracts with possible cosmetic and pharmaceutical applications. This study involved multiple reactions and reactants, such as Methyl heptanoate (cas: 106-73-0Electric Literature of C8H16O2).
Methyl heptanoate (cas: 106-73-0) belongs to esters. Esters perform as high-grade solvents for a broad array of plastics, plasticizers, resins, and lacquers, and are one of the largest classes of synthetic lubricants on the commercial market. Esterification is the general name for a chemical reaction in which two reactants (typically an alcohol and an acid) form an ester as the reaction product. Esters are common in organic chemistry and biological materials.Electric Literature of C8H16O2
Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics