In Vitro Tracking of Intracellular Metabolism-Derived Cancer Volatiles via Isotope Labeling was written by Lee, Dong-Kyu;Na, Euiyeon;Park, Seongoh;Park, Jeong Hill;Lim, Johan;Kwon, Sung Won. And the article was included in ACS Central Science in 2018.Synthetic Route of C15H22O2 The following contents are mentioned in the article:
Cancer detection relying on the release of volatile biomarkers has been extensively studied, but the individual biochem. processes of the cells from which biogenic volatiles originate have not been thoroughly elucidated to date. Inadequate determination of the metabolic origin of the volatile biomarkers has limited the progress of the scientific and practical applications of volatile biomarkers. To overcome the current limitations, the authors developed a metabolism tracking approach combining stable isotope labeling and flux anal. of volatiles to trace the intracellular metabolism-derived volatiles and to reveal their relation to cancer metabolic pathways. Specifically, after the 13C labeling of lung cancer cell, the isotopic ratio of whole cellular carbon was measured by nanoscale secondary ion mass spectrometry-based imaging. The kinetic modeling with the time-dependent isotopic ratio determined the period during which cancer cells reach the metabolic steady state, at which time all of the potential volatiles derived from intracellular metabolism were fully enriched isotopically. By measuring the isotopic enrichment of volatiles at the end-stage of isotopic flux, 2-pentadecanone appeared to be derived from the metabolic cascade starting from glucose to fatty acid synthesis. Furthermore, this biosynthetic pathway is distinct in cancer, as it was upregulated in colon, breast, and pancreatic cancer cells but not in normal cells. The study of the metabolic footprint of 2-pentadecanone demonstrates that the authors’ novel approach could be applied to trace the metabolic origin of biogenic volatile organic compounds This anal. strategy represents a potential cutting-edge tool in elucidating the biochem. authenticity of cancer volatiles and further expanding the authors’ understanding of the metabolic network of airborne metabolites in vitro. This study involved multiple reactions and reactants, such as 2-Ethylhexyl benzoate (cas: 5444-75-7Synthetic Route of C15H22O2).
2-Ethylhexyl benzoate (cas: 5444-75-7) belongs to esters. Esters are also usually derived from carboxylic acids. It may also be obtained by reaction of acid anhydride or acid halides with alcohols or by the reaction of salts of carboxylic acids with alkyl halides. Liquid esters of low volatility serve as softening agents for resins and plastics. Esters also include many industrially important polymers. Polymethyl methacrylate is a glass substitute sold under the names Lucite and Plexiglas; polyethylene terephthalate is used as a film (Mylar) and as textile fibres sold as Terylene, Fortrel, and Dacron.Synthetic Route of C15H22O2
Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics