Solvent-driven, self-assembled acid-responsive poly(ketalized serine)/siRNA complexes for RNA interference was written by Wong, Shirley;Kemp, Jessica A.;Shim, Min Suk;Kwon, Young Jik. And the article was included in Biomaterials Science in 2020.Quality Control of Diphenyl carbonate The following contents are mentioned in the article:
Advances in bionanotechnol. aim to develop smart nucleic acid delivery carriers with stimuli-responsive features to overcome challenges such as non-biodegradability, rapid clearance, immune response, and reaching intracellular targets. Peptide-based nanomaterials have become widely used in the field of gene and drug delivery due to their structural versatility and biomimetic properties. Particularly, polypeptide gene vectors that respond to biol. stimuli, such as acidic intracellular environments, have promising applications in mediating efficient endosomal escape and drug release. Unfortunately, synthesis strategies for efficient polymerization of acid-labile peptides have been limited due to conditions that fail to preserve acid-degradable functional groups. Stable urethane derivatives of the acid-labile amino acid ketalized serine (kSer) were synthesized and polymerized to a high mol. weight under permissive conditions independent of elevated temperature, restrictive solvents, or an inert atm. A new formulation strategy utilizing solvent-driven self-assembly of poly(kSer) peptides with small interfering RNA (siRNA) was developed, and the resulting poly(kSer)/siRNA complexes were further cross-linked for reinforced stability under physiol. conditions. The complexes were highly monodisperse and precisely spherical in morphol., which has significant clin. implications in definitive biodistribution, cellular internalization, and intracellular trafficking patterns. Self-assembled, cross-linked poly(kSer)/siRNA complexes demonstrated efficient nucleic acid encapsulation, internalization, endosomal escape, and acid-triggered cargo release, tackling multiple hurdles in siRNA delivery. The acid-responsive polypeptides and solvent-driven self-assembly strategies demonstrated in this study could be applicable to developing other efficient and safe delivery systems for gene and drug delivery. This study involved multiple reactions and reactants, such as Diphenyl carbonate (cas: 102-09-0Quality Control of Diphenyl carbonate).
Diphenyl carbonate (cas: 102-09-0) belongs to esters. Esters are also usually derived from carboxylic acids. It may also be obtained by reaction of acid anhydride or acid halides with alcohols or by the reaction of salts of carboxylic acids with alkyl halides. Acyl chlorides and acid anhydrides alcoholysis is another way to produce esters. Acyl chlorides and acid anhydrides react with alcohols to produce esters. Anydrous conditions are recommended since both acyl chlorides and acid anhydrides react with water.Quality Control of Diphenyl carbonate
Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics