In vitro anti-cancerous activity of 2-tridecanone extracted from Ruta chalepensis was written by Sathish, Vellaian;Kowsalya, Shanmugam;Umavathi, Subramaniam. And the article was included in Research Journal of Biotechnology in 2022.Recommanded Product: Octyl acetate The following contents are mentioned in the article:
Plants are rich source of therapeutic compounds that have tremendous applications in pharmaceutical industry. In recent years, cancer is a serious concern and large number of patients die due to cancer illnesses in spite of several interventions available. Development of effective and side effects lacking anticancer therapy is the trending research direction in healthcare pharmacy. The current investigation was carried out to confirm the outcomes of the methanolic extract of Ruta chalepensis leaves (Rutaceae) which inhibit the breast carcinoma cancer cell lines. R. chalepensis extract was carried out for the anti-cancerous activity for MCF-7 cells after treatment with various concentrations (0.1-1 mg/mL) of R. chalepensis extract for 48 h. The IC50 value of R. chalepensis extract on MCF-7 cells was determined by MTT assay as 493 卤 15.2 and 696.6 卤 11.5渭g/mL, resp. The morphol. changes that occurred in apoptotic cell were also perceived through Acridine Orange/ Ethidium Bromide (AO/EB) staining and this helped in deducing that the cell death observed was not due to necrosis, but due to apoptosis. The methanolic extract of R. chalepensis and 2-Tridecanone used at concentration of 0.5 mg/mL decreased the percentage of viable cells in MCF-7 cells; the percentage of apoptotic cells has increased to 42.46 and 46.68% for MCF -7 cells resp. compared to untreated cells. However, treatment of R. chalepensis and 2-Tridecanone significantly increased apoptosis as compared with control cells. The thin layer chromatog. (TLC) anal. used different concentrations of methanol and chloroform in 1:4 rations. Using the Rf formula, the present investigation on R. chalepensis was carried out and different compounds were identified such as alkaloids, flavonoids, ketones, sterols, phenols, amino acid and tannin among ketones. The FT-IR anal. obtained with the reference chart revealed the presence of ketones to identify the functional groups. In this investigation, isolation of 2-Tridecanone from R. chalepensis extracts was analyzed for GCMS anal. MS anal. revealed the presence of 2-Tridecanone compound The HPLC analyses to purified classes of increasing polarity. This study involved multiple reactions and reactants, such as Octyl acetate (cas: 112-14-1Recommanded Product: Octyl acetate).
Octyl acetate (cas: 112-14-1) belongs to esters. Carboxylic acid esters of low molecular weight are colourless, volatile liquids with pleasant odours, slightly soluble in water. Esters contain a carbonyl center, which gives rise to 120掳 C鈥揅鈥揙 and O鈥揅鈥揙 angles. Unlike amides, esters are structurally flexible functional groups because rotation about the C鈥揙鈥揅 bonds has a low barrier. Their flexibility and low polarity is manifested in their physical properties; they tend to be less rigid (lower melting point) and more volatile (lower boiling point) than the corresponding amides. Recommanded Product: Octyl acetate
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