Qin, Nan; Peng, Li-Yuan; Jin, Mei-Na; Wu, Xiao-Ran; Jia, Miao; Gan, Chun-Chun; Zhu, Wen; Zhang, Pan; Liu, Xin-Qi; Duan, Hong-Quan published an article in 2022. The article was titled 《Target identification of anti-diabetic and anti-obesity flavonoid derivative (Fla-CN)》, and you may find the article in Bioorganic Chemistry.HPLC of Formula: 51644-96-3 The information in the text is summarized as follows:
Fla-CN is a flavonoid derivative with anti-diabetic and anti-obesity effects; however, its biol. targets are still unknown. In this study, we developed bifunctional affinity-based probes to identify the direct targets of Fla-CN. When using probe 3, we observed the co-location of probe 3 and mitochondria in both HepG2 and 3T3-L1 cells. The putative target proteomes were obtained using activity-based protein profiling (ABPP) and photo-affinity labeling. Pyruvate carboxylase, mitochondrial malate dehydrogenase, mitochondrial complex I, and F1FO-ATPase were validated as the direct targets of Fla-CN by surface plasmon resonance (SPR) and biochem. assays. It was elucidated that the Tyr651, Gln870 and Lys912 were the key amino acid residues near the binding site of pyruvate carboxylase with Fla-CN. The direct interaction of Fla-CN and the above four targets allowed elucidation of its complicated mol. mechanism, including the activation of adenosine 5-monophosphate (AMP)-activated protein kinase (AMPK), and the inhibition of gluconeogenesis. Further investigation for activation of AMPK in normal and insulin resistance (IR) HepG2 cells, indicated that Fla-CN could target insulin resistance tissues. In the part of experimental materials, we found many familiar compounds, such as tert-Butyl (5-aminopentyl)carbamate(cas: 51644-96-3HPLC of Formula: 51644-96-3)
Some of the reported applications of tert-Butyl (5-aminopentyl)carbamate(cas: 51644-96-3) include: synthesis of of a supermacrocycle that self-assemble to form organic nanotubes., preparation of water-soluble unsymmetrical sulforhodamine fluorophores from monobrominated sulfoxanthene dye, synthesis of functionalized porphyrins as biocompatible carrier system for photodynamic therapy (PDT).HPLC of Formula: 51644-96-3
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