Zhang, Di; Yang, Bing; Chang, Shi-Quan; Ma, Sheng-Suo; Sun, Jian-Xin; Yi, Lin; Li, Xing; Shi, Hui-Mei; Jing, Bei; Zheng, Ya-Chun; Zhang, Chun-Lan; Chen, Feng-Guo; Zhao, Guo-Ping published the artcile< Protective effect of paeoniflorin on H2O2 induced Schwann cells injury based on network pharmacology and experimental validation>, SDS of cas: 112-63-0, the main research area is paeoniflorin neuroprotectant Schwann cell cytotoxicity apoptosis neurol disorder; H(2)O(2); Hydrogen peroxide; Network pharmacology; Paeoniflorin; Schwann cells; p38MAPK.
This study was to investigate the protective effect of paeoniflorin (PF) on hydrogen peroxide-induced injury. Firstly, “”SMILES”” of PF was searched in Pubchem and further was used for reverse mol. docking in Swiss Target Prediction database to obtain potential targets. Injury-related mols. were obtained from GeenCards database, and the predicted targets of PF for injury treatment were selected by Wayne diagram. For mechanism anal., the protein-protein interactions were constructed by String, and the KEGG anal. was conducted in Webgestalt. Then, cell viability and cytotoxicity assay were established by CCK8 assay. Also, the exptl. cells were allocated to control, model (200μmol·L-1 H2O2), SB203580 10μmol·L-1 (200μmol·L-1 H2O2+ SB203580 10μmol·L-1), PF 50μmol·L-1 (200μmol·L-1 H2O2+ PF 50μmol·L-1), and PF 100μmol·L-1 (200μmol·L-1 H2O2+ PF 100μmol·L-1) groups. We measured the intracellular ROS, Hoechst 33258 staining, cell apoptosis, the levels of Bcl-xl, Bcl-2, Caspase-3, Cleaved-caspase3, Cleaved-caspase7, TRPA1, TRPV1, and the phosphorylation expression of p38MAPK. There are 96 potential targets that may be associated with PF for injury treatment. Then, we chose the “”Inflammatory mediator regulation of TRP channels”” pathway for the exptl. verification from the first 10 KEGG pathway. In exptl. verification, H2O2 decreased the cell viability moderately (P < 0.05), and 100μmol·L -1 PF increased the cell viability significantly (P < 0.05). Depending on the difference of intracellular ROS fluorescence intensity, PF inhibited H 2O2-induced reactive oxygen species production in Schwann cells. In Hoechst 33258 staining, PF reversed the condensed chromatin and apoptotic nuclei following H2O2 treatment. Moreover, Flow cytometry results showed that PF could substantially inhibit H2O2 induced apoptosis (P < 0.05). Pretreatment with PF obviously reduced the levels of Caspase3, Cleaved-caspase3, Cleaved-caspase7, TRPA1, TRPV1, and the phosphorylation expression of p38MAPK after H 2O2 treatment (P < 0.05), increased the levels of Bcl-2, and Bcl-xl ( P < 0.05). PF inhibited Schwann cell injury and apoptosis induced by hydrogen peroxide, which mechanism was linked to the inhibition of phosphorylation of p38MAPK. Chinese Journal of Natural Medicines (Amsterdam, Netherlands) published new progress about Apoptosis. 112-63-0 belongs to class esters-buliding-blocks, and the molecular formula is C19H34O2, SDS of cas: 112-63-0.
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