Geahlen, Robert L.; Loudon, G. Marc; Paige, Lisa A.; Lloyd, David published the artcile< A general method for preparation of peptides biotinylated at the carboxy terminus>, HPLC of Formula: 112-63-0, the main research area is biotinylated peptide C terminus; Merrifield synthesis biotinylated peptide.
A method for the preparation of a biotinylated resin that can be elongated by standard methods of solid-phase peptide synthesis to give peptides biotinylated at the carboxy terminus is described. This methodol. is particularly important for the preparation of biotinylated peptides in which a free amino terminus is required. Coupling of Nε-9-fluorenylmethoxycarbonyl(Fmoc)-Nα-tert-butyloxycarbonyl(Boc)-L-lysine to p-methylbenzhydrylamine resin, followed by removal of the Fmoc protecting group and reaction with (+)-biotin-4-nitrophenyl ester yielded Nα-Boc-biocytin-p-methylbenzhydrylamine resin. The utility of this resin was tested by the synthesis of a biotinylated peptide, Gly-Asn-Ala-Ala-Ala-Ala-Arg-Arg-biocytin-NH2, for use as an in vitro substrate for myristoyl-CoA:protein N-myristoyltransferase (NMT), the enzyme that catalyzes protein N-myristoylation. Anal. of the peptide derivative by HPLC and mass spectrometry revealed a single major product of the expected mass, indicating that the biotin group survived cleavage and deprotection with HF. The biotinylated peptide served as a substrate for NMT, and the resulting myristoylated peptide could be quant. recovered by adsorption to immobilized avidin.
Analytical Biochemistry published new progress about Peptides Role: SPN (Synthetic Preparation), PREP (Preparation). 112-63-0 belongs to class esters-buliding-blocks, and the molecular formula is C19H34O2, HPLC of Formula: 112-63-0.
Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics