Rodenko, Boris; Toebes, Mireille; Hadrup, Sine Reker; van Esch, Wim J. E.; Molenaar, Annemieke M.; Schumacher, Ton N. M.; Ovaa, Huib published the artcile< Generation of peptide-MHC class I complexes through UV-mediated ligand exchange>, Computed Properties of 30095-98-8, the main research area is peptide complex MHC class I UV exchange.
Major histocompatibility complex (MHC) class I mols. present peptide ligands on the cell surface for recognition by appropriate cytotoxic T cells. MHC-bound peptides are critical for the stability of the MHC complex, and standard strategies for the production of recombinant MHC complexes are based on in vitro refolding reactions with specific peptides. This strategy is not amenable to high-throughput production of vast collections of MHC mols. The authors have developed conditional MHC ligands that form stable complexes with MHC mols. but can be cleaved upon UV irradiation The resulting empty, peptide-receptive MHC mols. can be charged with epitopes of choice under native conditions. Here the authors describe in-depth procedures for the high-throughput production of peptide-MHC (pMHC) complexes by MHC exchange, the anal. of peptide exchange efficiency by ELISA and the parallel production of MHC tetramers for T-cell detection. The production of the conditional pMHC complex by an in vitro refolding reaction can be achieved within 2 wk, and the actual high-throughput MHC peptide exchange and subsequent MHC tetramer formation require less than a day.
Nature Protocols published new progress about CD8-positive T cell. 30095-98-8 belongs to class esters-buliding-blocks, and the molecular formula is C9H9NO4, Computed Properties of 30095-98-8.
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