Preparation of poly(pentafluorophenyl acrylate) functionalized SiO2 beads for protein purification was written by Kim, Sura;Ku, Jayoung;Park, Jaemin;Kharbash, Raisa;Li, Sheng. And the article was included in Journal of Visualized Experiments in 2018.Application In Synthesis of Benzyl benzodithioate This article mentions the following:
We demonstrate a simple method to prepare poly(pentafluorophenyl acrylate) (poly(PFPA)) grafted silica beads for antibody immobilization and subsequent immunoprecipitation (IP) application. The poly(PFPA) grafted surface is prepared via a simple two-step process. In the first step, 3-aminopropyltriethoxysilane (APTES) is deposited as a linker mol. onto the silica surface. In the second step, poly(PFPA) homopolymer, synthesized via the reversible addition and fragmentation chain transfer (RAFT) polymerization, is grafted to the linker mol. through the exchange reaction between the pentafluorophenyl (PFP) units on the polymer and the amine groups on APTES. The deposition of APTES and poly(PFPA) on the silica particles are confirmed by XPS, as well as monitored by the particle size change measured via dynamic light scattering (DLS). To improve the surface hydrophilicity of the beads, partial substitution of poly(PFPA) with aminefunctionalized poly(ethylene glycol) (amino-PEG) is also performed. The PEG-substituted poly(PFPA) grafted silica beads are then immobilized with antibodies for IP application. For demonstration, an antibody against protein kinase RNA-activated (PKR) is employed, and IP efficiency is determined by Western blotting. The anal. results show that the antibody immobilized beads can indeed be used to enrich PKR while nonspecific protein interactions are minimal. In the experiment, the researchers used many compounds, for example, Benzyl benzodithioate (cas: 27249-90-7Application In Synthesis of Benzyl benzodithioate).
Benzyl benzodithioate (cas: 27249-90-7) belongs to esters. Volatile esters with characteristic odours are used in synthetic flavours, perfumes, and cosmetics. Certain volatile esters are used as solvents for lacquers, paints, and varnishes. Many esters have the potential for conformational isomerism, but they tend to adopt an s-cis (or Z) conformation rather than the s-trans (or E) alternative, due to a combination of hyperconjugation and dipole minimization effects. The preference for the Z conformation is influenced by the nature of the substituents and solvent, if present. Lactones with small rings are restricted to the s-trans (i.e. E) conformation due to their cyclic structure.Application In Synthesis of Benzyl benzodithioate
Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics