Synthesis of 1,2,4-triazole-linked urea/thiourea conjugates as cytotoxic and apoptosis inducing agents was written by Tokala, Ramya;Bale, Swarna;Janrao, Ingle Pavan;Vennela, Aluri;Kumar, Niggula Praveen;Senwar, Kishna Ram;Godugu, Chandraiah;Shankaraiah, Nagula. And the article was included in Bioorganic & Medicinal Chemistry Letters in 2018.Formula: C8H4N2O This article mentions the following:
A new series of 1,2,4-triazole-linked urea and thiourea conjugates have been synthesized and evaluated for their in vitro cytotoxicity against selected human cancer cell lines namely, breast (MCF-7, MDA-MB-231), lung (A549) prostate (DU145) and one mouse melanoma (B16-F10) cell line and compared with reference drug. The compound I showed significant cytotoxicity on MCF-7 breast cancer cell line with a IC50 value of 7.22 鍗?0.47 娓璏 among all the tested compounds Notably, induction of apoptosis by compound I on MCF-7 cells was evaluated using different staining techniques such as acridine orange/ethidium bromide (AO/EB), annexin V-FITC/PI, and DAPI. Further, clonogenic assay indicates the inhibition of colony formation on MCF-7 cells by compound I. Moreover, the flow-cytometric anal. also revealed that compound I caused the arrest of cells at G0/G1 phase of cell cycle. In addition, the compounds when tested on normal human cells (L-132) were found to be safer with low cytotoxicity profile. In the experiment, the researchers used many compounds, for example, 3-Cyanophenylisocyanate (cas: 16413-26-6Formula: C8H4N2O).
3-Cyanophenylisocyanate (cas: 16413-26-6) belongs to esters. Volatile esters with characteristic odours are used in synthetic flavours, perfumes, and cosmetics. Certain volatile esters are used as solvents for lacquers, paints, and varnishes. Acyl chlorides and acid anhydrides alcoholysis is another way to produce esters. Acyl chlorides and acid anhydrides react with alcohols to produce esters. Anydrous conditions are recommended since both acyl chlorides and acid anhydrides react with water.Formula: C8H4N2O
Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics