Site-directed mutagenesis identified the key active site residues of alcohol acyltransferase PpAAT1 responsible for aroma biosynthesis in peach fruits was written by Song, Zhi-zhong;Peng, Bin;Gu, Zi-xia;Tang, Mei-ling;Li, Bei;Liang, Mei-xia;Wang, Li-min;Guo, Xiao-tong;Wang, Jian-ping;Sha, Yu-fen;Zhang, Hong-xia. And the article was included in Horticulture Research in 2021.Reference of 706-14-9 This article mentions the following:
Abstract: The aroma of peach fruit is predominantly determined by the accumulation of γ-decalactone and ester compounds A previous study showed that the biosynthesis of these aroma compounds in peach fruit is catalyzed by PpAAT1, an alc. acyltransferase. In this work, we investigated the key active site residues responsible for γ-decalactone and ester biosynthesis. A total of 14 candidate amino acid residues possibly involved in internal esterification and 9 candidate amino acid residues possibly involved in esterification of PpAAT1 were assessed via site-directed mutagenesis. Analyzes of the in vitro enzyme activities of PpAAT1 and its site-directed mutant proteins (PpAAT1-SMs) with different amino acid residue mutations as well as the contents of γ-decalactone in transgenic tobacco leaves and peach fruits transiently expressing PpAAT1 and PpAAT1-SMs revealed that site-directed mutation of H165 in the conserved HxxxD motif led to lost enzymic activity of PpAAT1 in both internal esterification and its reactions, whereas mutation of the key amino acid residue D376 led to the total loss of γ-decalactone biosynthesis activity of PpAAT1. Mutations of 9 and 7 other amino acid residues also dramatically affected the enzymic activity of PpAAT1 in the internal esterification and esterification reactions, resp. Our findings provide a biochem. foundation for the mech. biosynthesis of γ-decalactone and ester compounds catalyzed by PpAAT1 in peach fruits, which could be used to guide the mol. breeding of new peach species with more favorable aromas for consumers. In the experiment, the researchers used many compounds, for example, 5-Hexyldihydrofuran-2(3H)-one (cas: 706-14-9Reference of 706-14-9).
5-Hexyldihydrofuran-2(3H)-one (cas: 706-14-9) belongs to esters. Esters perform as high-grade solvents for a broad array of plastics, plasticizers, resins, and lacquers, and are one of the largest classes of synthetic lubricants on the commercial market. Polyesters are important plastics, with monomers linked by ester moieties. Esterification is the general name for a chemical reaction in which two reactants (typically an alcohol and an acid) form an ester as the reaction product. Esters are common in organic chemistry and biological materials.Reference of 706-14-9
Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics