Elution behavior of a 3D-printed, milled and conventional resin-based occlusal splint material was written by Wedekind, Lennart;Gueth, Jan-Frederik;Schweiger, Josef;Kollmuss, Maximilian;Reichl, Franz-Xaver;Edelhoff, Daniel;Hoegg, Christof. And the article was included in Dental Materials in 2021.Application of 84-61-7 This article mentions the following:
The elution of unpolymerized (co-)monomers and additives from methacrylic resin-based materials like polymethyl methacrylate (PMMA) can cause adverse side effects, such as mutagenicity, teratogenicity, genotoxicity, cytotoxicity and estrogenic activity.The aim of this study was to quantify the release and the cytotoxicity of residual (co-)monomers and additives from PMMA-based splint materials under consideration of real splint sizes. Three different materials used for additive (3D printing), subtractive (milling) and conventional (powder and liquid) manufacturing were examinedThe splint materials SHERAprint-ortho plus (additive), SHERAeco-disk PM20 (subtractive) and SHERAORTHOMER (conventional) were analyzed. 16 (n = 4) sample disks of each material (6 mm diameter and 2 mm height) were polished on the circular and one cross-section area and then eluted in both distilled water and methanol. The disks were incubated at 37°C for 24 h or 72 h and subsequently analyzed by gas chromatog./mass spectrometry (GC/MS) for specifying and quantifying released compounds XTT-based cell viability assays with human gingival fibroblasts (HGFs) were performed for Tetrahydrofurfuryl methacrylate (THFMA), 1,4-Butylene glycol dimethacrylate (BDDMA) and Tripropylenglycol diacrylate (TPGDA). In order to project the disk size to actual splint sizes in a worst-case scenario, lower and upper jaw occlusal splints were designed and volumes and surfaces were measured.For SHERAeco-disk PM20 and for SHERAORTHOMER no elution was determined in water. SHERAprint-ortho plus eluted the highest THFMA concentration of 7.47μ mol/l ±2,77μmol/l after 72 h in water. Six (co-)monomers and five additives were detected in the methanol eluates of all three materials tested. The XTT-based cell viability assays resulted in a EC50 of 3006 ± 408μmol/l for THFMA, 2569.5 ± 308μmol/l for BDDMA and 596.7 ± 88μmol/l for TPGDA.With the solvent methanol, released components from the investigated splint materials exceeded cytotoxic concentrations in HGFs calculated for a worst-case scenario in splint size. In the water eluates only the methacrylate THFMA could be determined from SHERAprint-ortho plus in concentrations below cytotoxic levels in HGFs. In the experiment, the researchers used many compounds, for example, Dicyclohexyl phthalate (cas: 84-61-7Application of 84-61-7).
Dicyclohexyl phthalate (cas: 84-61-7) belongs to esters. Volatile esters with characteristic odours are used in synthetic flavours, perfumes, and cosmetics. Certain volatile esters are used as solvents for lacquers, paints, and varnishes. Because of their lack of hydrogen-bond-donating ability, esters do not self-associate. Consequently, esters are more volatile than carboxylic acids of similar molecular weight.Application of 84-61-7
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