Zhou, Biting published the artcileProtective Effects of Nicotinamide Riboside on H2O2-induced Oxidative Damage in Lens Epithelial Cells, Synthetic Route of 2044-85-1, the main research area is epithelial cell oxidative damage nicotinamide riboside hydrogen peroxide; Nicotinamide riboside; apoptosis; lens epithelial cell; mitochondria; oxidative stress.
To investigate the protective effects of nicotinamide riboside (NR) on oxidative damage in hydrogen peroxide (H2O2)-exposed human lens epithelial cell lines and possible mechanisms underlying its protective effects: SRA01/04 cells were divided into three groups:control group, model group and treatment group. Superoxide dismutase,catalase and total glutathione levels were detected to evaluate oxidative damage induced by diff. concentrations of H2O2 in SRA01/04 cells. After SRA01/04 cells were treated with NR and/or H2O2, cell viability was evaluated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and Hoechst staining, cell apoptosis was analyzed using flow cytometry, reactive oxygen species were measured with DCFH-DA probe, mitochondria were stained with MitoTracker to measure mitochondrial membrane potential. In addition, western blotting was performed to detect levels of proteins associated with apoptosis and related pathways: H2O2 induced oxidative damage in SRA01/04 cells by inhibiting activity of SOD and CAT and reducing total GSH levels. Furthermore, NR significantly inhibited the activation of the MAPK pathway but promoted activation of the JAK2/Stat3 pathway compared with the model group:NR may alleviate oxidative damage by targeting MAPK and JAK2/Stat3 pathways in H2O2-treated SRA01/04 cells.
Current Eye Research published new progress about Age-related cataract. 2044-85-1 belongs to class esters-buliding-blocks, name is 2′,7′-Dichloro-3-oxo-3H-spiro[isobenzofuran-1,9′-xanthene]-3′,6′-diyl diacetate, and the molecular formula is C24H14Cl2O7, Synthetic Route of 2044-85-1.
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