Chen, Cheng published the artcileAsymmetric reduction of 2-chloro-3-oxo-ester into enantiomerically high pure diltiazem precursor by a Candida ketoreductase, Application In Synthesis of 5405-41-4, the main research area is Candida ketoreductase asym reduction oxoester chiral synthon diltiazem.
Me (2R,3S)-3-(4-methoxyphenyl)glycidate [(2R,3S)-MPGM] is an advanced chiral synthon for the synthesis of the cardiovascular drug diltiazem. It can be easily accessed by cyclizing the reduction products of Me 2-chloro-3-(4-methoxyphenyl)-3-oxo-propanoate. Herein, we report an identified carbonyl reductase (CpKR) from Candida parapsilosis that displayed an excellent stereoselectivity toward the keto substituent at the C3-position of the 2-chloro-3-oxo-ester. The engineered Escherichia coli cells harboring CpKR gene were directly applied for the asym. reduction of keto ester 1a with a space-time yield of 46 g L-1 d-1, which represents the highest productivity in bio-reduction reported so far. The isolated chiral alc. products were then applied to the chem. synthesis of (2R,3S)-MPGM in 99% ee and a total yield of 76% in the two-step chemo-enzymic reactions, which far exceeded the maximum theor. yield (50%) of the existing industrial process based on a lipase-catalyzed resolution of racemic MPGM. This work provides a promising eco-friendly and cost-effective route toward industrial synthesis of pharmaceutically relevant diltiazem.
Molecular Catalysis published new progress about Candida parapsilosis. 5405-41-4 belongs to class esters-buliding-blocks, name is Ethyl 3-hydroxybutanoate, and the molecular formula is C6H12O3, Application In Synthesis of 5405-41-4.
Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics