Dodgson, K. S. published the artcileSulfatase. XI. The purification and properties of the arylsulfatase of Alcaligenes met-alcaligenes, Formula: C6H4KNO6S, the publication is Biochemical Journal (1955), 374-80, database is CAplus and MEDLINE.
cf. C.A. 49, 13305c. The arylsulfatase of A. met-alcaligenes was concentrated and partially separated from nucleic acid and from other enzymes by paper electrophoresis. The enzyme concentrate gave pH-activity and substrate concentration-activity curves which were very similar to those obtained previously with the stage B enzyme (loc. cit.). The ratio of the activities towards the 3 substrates under the resp. optimum conditions was 5.2 p-acetylphenyl (APS), 5.0 p-nitrophenyl (NPS), and 1.0 nitrocatechol (NCS) sulfates, calculated on a molar basis. When account was taken of the inhibition by excess of substrate and the true Vmaximum calculated the ratio became 15.1:14.4:1.0. The Michaelis constants, Km, for the 3 substrates were 9.0, 4.75, and 2.2 × 10-4M, resp. The heat of activation with NPS was 11 kcal./mol. Inhibition was obtained with the following substances at 0.025M: NaHSO3 17%, NaCN 82, NaHSO3 34, BaCl2 100, glucose 6-sulfate 17, K Et sulfate 12, Na benzenesulfonate 22, phenylhydrazine (0.0001 M) 87, hydrazine 43, semicarbazide 21, thiosemicarbazide 33, dimedone (0.05%, weight/volume) 27, Girards’ reagent P (2.5% weight/volume) 25, NH2OH 97. Inhibition by p-chloromercuribenzoic acid could not be reversed by cysteine or glutathione. 2,4-Dinitrofluorobenzene at 0.005M gave 100% inhibition. The curves obtained by plotting pKm against pH have shown the presence in the enzyme of 2 different substrate-binding groups: pK 8.2 and 9.4.
Biochemical Journal published new progress about 6217-68-1. 6217-68-1 belongs to esters-buliding-blocks, auxiliary class Salt,Nitro Compound,Sulfonate,Benzene, name is Potassium 4-nitrophenyl sulfate, and the molecular formula is C6H4KNO6S, Formula: C6H4KNO6S.
Referemce:
https://en.wikipedia.org/wiki/Ester,
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