Jeon, Byoungjun published the artcileEnhanced predictive capacity using dual-parameter chip model that simulates physiological skin irritation, Application of Hexyl 2-hydroxybenzoate, the main research area is chip model skin irritation endothelium toxicity; Alternative to animal testing; Organ on a chip; Reconstructed human epidermis; Skin irritation; Skin on a chip; Toxicity.
Current alternatives to animal testing methods for skin irritation evaluation such as reconstructed human epidermis models are not fully representing physiol. response caused by skin irritants. Skin irritation is physiol. induced by the dilation and increased permeability of endothelial cells. Thus, our objectives were to mimic physiol. skin irritation using a skin-on-a-chip model and compare predictive capacities with a reconstructed human epidermis model to evaluate its effectiveness. To achieve our goals, the skin-on-a-chip model, consisting of three layers representing the epidermal, dermal and endothelial components, was adapted. Cell viability was measured using the OECD TG 439 protocol for test substance evaluation. The tight junctions of endothelial cells were also observed and measured to assess physiol. responses to test substances. These parameters were used to physiol. evaluate cell-to-cell interactions induced by test substances and quantify model accuracy, sensitivity, and specificity. Based on in vivo data, the classification accuracy of twenty test substances using a dual-parameter chip model was 80%, which is higher than other methods. Besides, the chip model was more suitable for simulating human skin irritation. Therefore, it is important to note that the dual-parameter chip model possesses an enhanced predictive capacity and could serve as an alternative to animal testing for skin irritation.
Toxicology In Vitro published new progress about Endothelium. 6259-76-3 belongs to class esters-buliding-blocks, name is Hexyl 2-hydroxybenzoate, and the molecular formula is C13H18O3, Application of Hexyl 2-hydroxybenzoate.
Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics