do Nascimento, Fernando H. published the artcileFast construction of polymer monolithic columns inside fluorinated ethylene propylene (FEP) tubes for separation of proteins by reversed-phase liquid chromatography, Recommanded Product: Dodecyl 2-methylacrylate, the main research area is polymer monolithic column fluorinated ethylene propylene protein RPHPLC; Egg white; Fluoropolymer tubes; Liquid chromatography; Monolithic columns; Photografting; Urine.
This paper describes the preparation of polymer monolithic columns in the confines of fluorinated ethylene propylene (FEP) tubes. These tubes are cheap, chem. stable, and widely used in flow anal. laboratories UV-initiated grafting with 5% benzophenone in methanol for 1 h activated the internal surface walls, thus enabling the further covalent binding of ethylene glycol dimethacrylate (EDMA) from a 15% solution in methanol, also via photografting. Both steps used 254 nm radiation under a potency of 120 mJ cm2. ATR-FTIR measurements revealed carbonyl, alkyl and vinyl groups in the functionalized FEP. The d. of vinyl groups was high enough to firmly attach a poly(lauryl methacrylate-coethylene glycol dimethacrylate) monolith in 120 × 1.57 mm internal diameter tubes, prepared via photopolymerization The total preparation lasts <2-h. The columns were permeable, (1.58 ± 0.06) x 10-13 m2, providing reproducible chromatog. parameters of retention times, retention factor, selectivity, and resolution The monoliths were stable at flow rates of 500μL min-1, collapsing only at flow rates >700μL min-1, a condition that increased the backpressure over 1000 psi (experiments at the room temperature). The separation of proteins by reversed-phase liquid chromatog. demonstrated the efficiency of the columns. Determination of egg white proteins (ovalbumin and lysozyme) and myoglobin in spiked urine proved the applicability to the anal. of real samples.
Talanta published new progress about Egg white, poultry. 142-90-5 belongs to class esters-buliding-blocks, name is Dodecyl 2-methylacrylate, and the molecular formula is C16H30O2, Recommanded Product: Dodecyl 2-methylacrylate.
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