Haval, Gauri A. published the artcileExcess hydrogen peroxide inhibits head and foot regeneration in hydra by affecting DNA repair and expression of essential genes, Safety of 2′,7′-Dichloro-3-oxo-3H-spiro[isobenzofuran-1,9′-xanthene]-3′,6′-diyl diacetate, the main research area is foot hydrogen peroxide DNA repair; hydra; reactive oxygen species; regeneration.
ROS are necessary for various cellular processes. However, excess ROS cause damage to many biol. mols. and therefore must be tightly regulated in time and space. H2O2 is the most commonly used ROS as second messenger in the cell. It is a relatively long-lived freely diffusible signaling mol. during early events of injury. In the Cnidarian hydra, injury-induced ROS production is essential for regeneration to proceed. In the present study, we have examined influence of varying exposure to H2O2 on head and foot regeneration in the middlepieces of trisected hydra. We find that longer (4 h) exposure to 1 mM H2O2 inhibits both head and foot regeneration while shorter exposure (2 h) does not. Longer exposure to H2O2 resulted in extensive damage to DNA that could not be repaired, probably due to suboptimal induction of APE1, an enzyme necessary for base excision repair (BER). Concomitantly, genes involved in activation of Wnt pathway, necessary for head regeneration, were significantly downregulated. This appeared to be due to failure of both stabilization and transient nuclear localization of β-catenin. Similarly, genes involved in foot regeneration were also downregulated on longer exposure to H2O2. Thus, exposure to excess ROS inhibits regenerative processes in hydra through reduced expression of genes involved in regeneration and diminished DNA repair.
Journal of Biochemical and Molecular Toxicology published new progress about Animal gene Role: BSU (Biological Study, Unclassified), BIOL (Biological Study) (APE1). 2044-85-1 belongs to class esters-buliding-blocks, name is 2′,7′-Dichloro-3-oxo-3H-spiro[isobenzofuran-1,9′-xanthene]-3′,6′-diyl diacetate, and the molecular formula is C24H14Cl2O7, Safety of 2′,7′-Dichloro-3-oxo-3H-spiro[isobenzofuran-1,9′-xanthene]-3′,6′-diyl diacetate.
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