Zhang, Chi published the artcileSite-Selective Cysteine-Cyclooctyne Conjugation, Computed Properties of 882847-32-7, the main research area is cysteine cyclooctyne conjugation DBCO tag peptide; bioconjugation; bioorthogonal chemistry; cysteine-cyclooctyne reaction; dibenzocyclooctyne; protein modification.
The authors report a site-selective cysteine-cyclooctyne conjugation reaction between a seven-residue peptide tag (DBCO-tag, Leu-Cys-Tyr-Pro-Trp-Val-Tyr) at the N or C terminus of a peptide or protein and various aza-dibenzocyclooctyne (DBCO) reagents. Compared to a cysteine peptide control, the DBCO-tag increases the rate of the thiol-yne reaction 220-fold, thereby enabling selective conjugation of DBCO-tag to DBCO-linked fluorescent probes, affinity tags, and cytotoxic drug mols. Fusion of DBCO-tag with the protein of interest enables regioselective cysteine modification on proteins that contain multiple endogenous cysteines; these examples include green fluorescent protein and the antibody trastuzumab. Short peptide tags can aid in accelerating bond-forming reactions that are often slow to non-existent in water.
Angewandte Chemie, International Edition published new progress about Enhanced green fluorescent proteins Role: BSU (Biological Study, Unclassified), PEP (Physical, Engineering or Chemical Process), BIOL (Biological Study), PROC (Process) (fusion protein with DCBO-tag). 882847-32-7 belongs to class esters-buliding-blocks, name is 1-(9H-Fluoren-9-yl)-3-oxo-2,7,10,13,16,19-hexaoxa-4-azadocosan-22-oic acid, and the molecular formula is C28H37NO9, Computed Properties of 882847-32-7.
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