Fast filtration with a vacuum manifold system as a rapid and robust metabolome sampling method for Saccharomyces cerevisiae was written by Yun, Eun Ju;Seo, Seung-Oh;Kwak, Suryang;Oh, Eun Joong;Lee, Sun-Hee;Jin, Yong-Su;Kim, Kyoung Heon. And the article was included in Process Biochemistry (Oxford, United Kingdom) in 2021.Product Details of 31566-31-1 The following contents are mentioned in the article:
For metabolome sample preparation, rapid quenching of cellular metabolism without severe loss of intracellular metabolites is important. Here, we comparatively evaluated the two sampling methods, cold methanol quenching and fast filtration, for one of the most important industrial microorganisms, yeast Saccharomyces cerevisiae. The latter, using a vacuum manifold system capable of processing up to twenty samples simultaneously, was developed here. The results showed that the cold methanol quenching method resulted in severe losses of intracellular metabolites but that sugars and sugar phosphates were highly preserved. In comparison, the fast filtration method better preserved most metabolites with less extracellular contamination. Our results suggested that the fast filtration method with a vacuum manifold system can be widely applied to yeast metabolome anal. due to its simplicity, robustness, and lack of significant intracellular metabolite loss. This study involved multiple reactions and reactants, such as Glyceryl monostearate (cas: 31566-31-1Product Details of 31566-31-1).
Glyceryl monostearate (cas: 31566-31-1) belongs to esters. Esters are widespread in nature and are widely used in industry. In nature, fats are in general triesters derived from glycerol and fatty acids. Esters are responsible for the aroma of many fruits. Liquid esters of low volatility serve as softening agents for resins and plastics. Esters also include many industrially important polymers. Polymethyl methacrylate is a glass substitute sold under the names Lucite and Plexiglas; polyethylene terephthalate is used as a film (Mylar) and as textile fibres sold as Terylene, Fortrel, and Dacron.Product Details of 31566-31-1
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