Solubilization of artificial mitochondrial membranes by amphiphilic copolymers of different charge was written by Janson, Kevin;Zierath, Jennifer;Kyrilis, Fotis L.;Semchonok, Dmitry A.;Hamdi, Farzad;Skalidis, Ioannis;Kopf, Adrian H.;Das, Manabendra;Kolar, Cenek;Rasche, Marie;Vargas, Carolyn;Keller, Sandro;Kastritis, Panagiotis L.;Meister, Annette. And the article was included in Biochimica et Biophysica Acta, Biomembranes in 2021.Reference of 26662-94-2 The following contents are mentioned in the article:
Certain amphiphilic copolymers form lipid-bilayer nanodiscs from artificial and natural membranes, thereby rendering incorporated membrane proteins optimal for structural anal. Recent studies have shown that the amphiphilicity of a copolymer strongly determines its solubilization efficiency. This is especially true for highly neg. charged membranes, which experience pronounced Coulombic repulsion with polyanionic polymers. Here, we present a systematic study on the solubilization of artificial multicomponent lipid vesicles that mimic inner mitochondrial membranes, which harbor essential membrane-protein complexes. In particular, we compared the lipid-solubilization efficiencies of established anionic with less densely charged or zwitterionic and even cationic copolymers in low- and high-salt concentrations The nanodiscs formed under these conditions were characterized by dynamic light scattering and neg.-stain electron microscopy, pointing to a bimodal distribution of nanodisc diameters with a considerable fraction of nanodiscs engaging in side-by-side interactions through their polymer rims. Overall, our results show that some recent, zwitterionic copolymers are best suited to solubilize neg. charged membranes at high ionic strengths even at low polymer/lipid ratios. This study involved multiple reactions and reactants, such as (2R,9Z)-1-(((2-Aminoethoxy)(hydroxy)phosphoryl)oxy)-3-(palmitoyloxy)propan-2-yl oleate (cas: 26662-94-2Reference of 26662-94-2).
(2R,9Z)-1-(((2-Aminoethoxy)(hydroxy)phosphoryl)oxy)-3-(palmitoyloxy)propan-2-yl oleate (cas: 26662-94-2) belongs to esters. Volatile esters with characteristic odours are used in synthetic flavours, perfumes, and cosmetics. Certain volatile esters are used as solvents for lacquers, paints, and varnishes. Esters contain a carbonyl center, which gives rise to 120° C–C–O and O–C–O angles. Unlike amides, esters are structurally flexible functional groups because rotation about the C–O–C bonds has a low barrier. Their flexibility and low polarity is manifested in their physical properties; they tend to be less rigid (lower melting point) and more volatile (lower boiling point) than the corresponding amides. Reference of 26662-94-2
Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics