Volatolomics approach by HS-SPME-GC-MS and multivariate analysis to discriminate olive tree varieties infected by Xylella fastidiosa was written by Mentana, Annalisa;Camele, Ippolito;Mang, Stefania M.;De Benedetto, Giuseppe E.;Frisullo, Salvatore;Centonze, Diego. And the article was included in Phytochemical Analysis in 2019.Safety of Methyl heptanoate The following contents are mentioned in the article:
Xylella fastidiosa (Xf) is a pathogenic bacterium that causes diseases in olive trees. Therefore, anal. methods for both the characterization of the host/pathogen interaction and infection monitoring are needed. Volatile organic compounds (VOCs) are emitted by plants relate to their physiol. state, therefore VOCs monitoring can assist in detecting stress or infection states before visible signs are present. In this work, the headspace-solid phase microextraction-gaschromatog.-mass spectrometry (HS-SPME-GC-MS) technique was used for the first time to highlight VOCs differences between healthy and Xf-infected olive trees. VOCs from olive tree twig samples were extracted and analyzed by HS-SPME-GC-MS, and hence identified by comparing the exptl. linear retention indexes with the reference values and by MS data obtained from NIST library. Data were processed by principal component anal. (PCA) and anal. of variance (ANOVA). The HS-SPME step was optimized in terms of adsorbent phase and extraction time. HS-SPME-GC-MS technique was applied to the extraction and anal. of VOCs of healthy and Xf-infected olive trees. More than 100 compounds were identified and the differences between samples were evidenced by the multivariate anal. approach. The results showed the marked presence of Me esters in Xf-infected samples, suggesting their probable involvement in the mechanism of diffusible signal factor. The proposed approach represents an easy and solvent-free method to evaluate the presence of Xf in olive trees, and to evidence volatiles produced by host/pathogen interactions that could be involved in the defensive mechanism of the olive tree and/or in the infective action of Xf. This study involved multiple reactions and reactants, such as Methyl heptanoate (cas: 106-73-0Safety of Methyl heptanoate).
Methyl heptanoate (cas: 106-73-0) belongs to esters. Esters perform as high-grade solvents for a broad array of plastics, plasticizers, resins, and lacquers, and are one of the largest classes of synthetic lubricants on the commercial market. Liquid esters of low volatility serve as softening agents for resins and plastics. Esters also include many industrially important polymers. Polymethyl methacrylate is a glass substitute sold under the names Lucite and Plexiglas; polyethylene terephthalate is used as a film (Mylar) and as textile fibres sold as Terylene, Fortrel, and Dacron.Safety of Methyl heptanoate
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Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics