Solid phase microextraction coupled with comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry for high-resolution metabolite profiling in apples: Implementation of structured separations for optimization of sample preparation procedure in complex samples was written by Risticevic, Sanja;DeEll, Jennifer R.;Pawliszyn, Janusz. And the article was included in Journal of Chromatography A in 2012.Related Products of 5444-75-7 The following contents are mentioned in the article:
Metabolomics currently represents one of the fastest growing high-throughput mol. anal. platforms that refer to the simultaneous and unbiased anal. of metabolite pools constituting a particular biol. system under investigation. In response to the ever increasing interest in development of reliable methods competent with obtaining a complete and accurate metabolomic snapshot for subsequent identification, quantification and profiling studies, the purpose of the current investigation is to test the feasibility of solid phase microextraction for advanced fingerprinting of volatile and semivolatile metabolites in complex samples. In particular, the current study is focussed on the development and optimization of solid phase microextraction (SPME) – comprehensive two-dimensional gas chromatog.-time-of-flight mass spectrometry (GC 脳 GC-ToFMS) methodol. for metabolite profiling of apples (Malus 脳domestica Borkh.). For the first time, GC 脳 GC attributes in terms of mol. structure-retention relationships and utilization of two-dimensional separation space on orthogonal GC 脳 GC setup were exploited in the field of SPME method optimization for complex sample anal. Anal. performance data were assessed in terms of method precision when com. coatings are employed in spiked metabolite aqueous sample anal. The optimized method consisted of the implementation of direct immersion SPME (DI-SPME) extraction mode and its application to metabolite profiling of apples, and resulted in a tentative identification of 399 metabolites and the composition of a metabolite database far more comprehensive than those obtainable with classical one-dimensional GC approaches. Considering that specific metabolome constituents were for the first time reported in the current study, a valuable approach for future advanced fingerprinting studies in the field of fruit biol. is proposed. The current study also intensifies the understanding of SPME-GC 脳 GC-ToFMS hyphenation and outlines the benefits of facilitating GC 脳 GC for SPME method optimization. The obtained results clearly illustrate that acquisition of a more complete metabolome snapshot is only attainable under optimized conditions for both techniques. This study involved multiple reactions and reactants, such as 2-Ethylhexyl benzoate (cas: 5444-75-7Related Products of 5444-75-7).
2-Ethylhexyl benzoate (cas: 5444-75-7) belongs to esters. Esters are also usually derived from carboxylic acids. It may also be obtained by reaction of acid anhydride or acid halides with alcohols or by the reaction of salts of carboxylic acids with alkyl halides. Acyl chlorides and acid anhydrides alcoholysis is another way to produce esters. Acyl chlorides and acid anhydrides react with alcohols to produce esters. Anydrous conditions are recommended since both acyl chlorides and acid anhydrides react with water.Related Products of 5444-75-7
Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics