Distinct lipid membrane interaction and uptake of differentially charged nanoplastics in bacteria was written by Dai, Shang;Ye, Rui;Huang, Jianxiang;Wang, Binqiang;Xie, Zhenming;Ou, Xinwen;Yu, Ning;Huang, Cheng;Hua, Yuejin;Zhou, Ruhong;Tian, Bing. And the article was included in Journal of Nanobiotechnology in 2022.Recommanded Product: 26662-94-2 The following contents are mentioned in the article:
Nanoplastics have been recently found widely distributed in our natural environment where ubiquitously bacteria are major participants in various material cycles. Understanding how nanoplastics interact with bacterial cell membrane is critical to grasp their uptake processes as well as to analyze their associated risks in ecosystems and human microflora. However, little is known about the detailed interaction of differentially charged nanoplastics with bacteria. The present work exptl. and theor. demonstrated that nanoplastics enter into bacteria depending on the surface charges and cell envelope structural features, and proved the shielding role of membrane lipids against nanoplastics. Pos. charged polystyrene nanoplastics (PS-NH2, 80 nm) can efficiently translocate across cell membranes, while neg. charged PS (PS-COOH) and neutral PS show almost no or much less efficacy in translocation. Mol. dynamics simulations revealed that the PS-NH2 displayed more favorable electrostatic interactions with bacterial membranes and was subjected to internalisation through membrane penetration. The pos. charged nanoplastics destroy cell envelope of Gram-pos. B. subtilis by forming membrane pore, while enter into the Gram-neg. E. coli with a relatively intact envelope. The accumulated pos. charged nanoplastics conveyed more cell stress by inducing a higher level of reactive oxygen species (ROS). However, the subsequently released membrane lipid-coated nanoplastics were nearly nontoxic to cells, and like wise, stealthy bacteria wrapped up with artificial lipid layers became less sensitive to the pos. charged nanoplastics, thereby illustrating that the membrane lipid can shield the strong interaction between the pos. charged nanoplastics and cells. Our findings elucidated the mol. mechanism of nanoplastics interaction and accumulation within bacteria, and implied the shielding and internalization effect of membrane lipid on toxic nanoplastics could promote bacteria for potential plastic bioremediation. This study involved multiple reactions and reactants, such as (2R,9Z)-1-(((2-Aminoethoxy)(hydroxy)phosphoryl)oxy)-3-(palmitoyloxy)propan-2-yl oleate (cas: 26662-94-2Recommanded Product: 26662-94-2).
(2R,9Z)-1-(((2-Aminoethoxy)(hydroxy)phosphoryl)oxy)-3-(palmitoyloxy)propan-2-yl oleate (cas: 26662-94-2) belongs to esters. Esters are widespread in nature and are widely used in industry. In nature, fats are in general triesters derived from glycerol and fatty acids. Esters are responsible for the aroma of many fruits. Esters contain a carbonyl center, which gives rise to 120掳 C鈥揅鈥揙 and O鈥揅鈥揙 angles. Unlike amides, esters are structurally flexible functional groups because rotation about the C鈥揙鈥揅 bonds has a low barrier. Their flexibility and low polarity is manifested in their physical properties; they tend to be less rigid (lower melting point) and more volatile (lower boiling point) than the corresponding amides. Recommanded Product: 26662-94-2
Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics