Sugar based cationic magnetic core-shell silica nanoparticles for nucleic acid extraction was written by Ali, Tammar Hussein;Mandal, Amar Mousa;Heidelberg, Thorsten;Hussen, Rusnah Syahila Duali. And the article was included in RSC Advances in 2022.COA of Formula: C16H22O11 The following contents are mentioned in the article:
Nucleic acid (NA) extraction is an essential step in mol. testing for a wide range of applications. Conventional extraction protocols usually suffer from time consuming removal of non-nucleic acid impurities. In this study, a new magnetic nanoparticle (MNP) is presented to simplify the NA extraction A core-shell design, comprising of a ferromagnetic core coated with mesoporous silica, forms the basis of the functional nanoparticle. Chem. functionalization of the silica coating includes a multistep synthesis, in which an activated nanoparticle is coupled with a triethylene glycol spaced glycosyl imidazole. The mol. design aims for charge interactions between the imidazolium-based pos. nanoparticle surface and nucleic acids, with specific hydrogen bonding between the surface bonded carbohydrate and nucleic acid targets to ensure nucleic acid selectivity and avoid protein contamination. Two different carbohydrates, differing in mol. size, were selected to compare the efficiency in terms of NA extraction A triethylene glycol spacer provides sufficient flexibility to remove particle surface constraints for the interaction. The Brunauer-Emmett-Teller (BET) anal. shows a significantly larger surface area for the disaccharide-based particles NpFeSiImMalt (鈭?81 m2 g-1) compared to the monosaccharide analog NpFeSiImGlc (鈭?16 m2 g-1) at small particles sizes (range 鈭?15 nm) and sufficient magnetization (29 emu g-1) for easy isolation by an external magnetic field. The particles enabled a high DNA particle loading ratio of 30-45 wt% (MNP/DNA ratio), reflecting an efficient extraction process. A high desorption rate (7 min) with more than 86% of unchanged DNA loading was recorded, indicating low damage to the target extract This study involved multiple reactions and reactants, such as (2S,3R,4S,5R,6R)-6-(Acetoxymethyl)tetrahydro-2H-pyran-2,3,4,5-tetrayl tetraacetate (cas: 604-69-3COA of Formula: C16H22O11).
(2S,3R,4S,5R,6R)-6-(Acetoxymethyl)tetrahydro-2H-pyran-2,3,4,5-tetrayl tetraacetate (cas: 604-69-3) belongs to esters. Esters perform as high-grade solvents for a broad array of plastics, plasticizers, resins, and lacquers, and are one of the largest classes of synthetic lubricants on the commercial market. Because of their lack of hydrogen-bond-donating ability, esters do not self-associate. Consequently, esters are more volatile than carboxylic acids of similar molecular weight.COA of Formula: C16H22O11
Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics