Effects of the timing of a culture temperature reduction on the comprehensive metabolite profiles of Chlorella vulgaris was written by Ahn, Hye Min;Kim, So-Hyun;Hyun, Sun-Hee;Lim, Sa Rang;Kim, Hye-Youn;Oh, Junsang;Lee, Kyung-Min;Hong, Seong-Joo;Cho, Byung-Kwan;Lee, Hookeun;Lee, Choul-Gyun;Choi, Hyung-Kyoon. And the article was included in Journal of Applied Phycology in 2016.Application In Synthesis of Methyl nonadecanoate The following contents are mentioned in the article:
This study investigated the effects of different temperature conditions on the comprehensive metabolite profiles of Chlorella vulgaris using gas chromatog.-mass spectrometry coupled with multivariate statistical anal. C. vulgaris cells cultivated at 20 掳C were transferred to 10 掳C incubators at different time points of cultivation [days 0 (TR0D), 7 (TR7D), and 14 (TR14D)], then they were cultivated at 10 掳C until harvesting at day 21 to compare the growth and comprehensive metabolite profiles with those cultivated under a constant cultivation temperature of 20 掳C (T20). There was no significant difference in algal cell growth between cultivation under the T20 and temperature reduction (TR) conditions. Algal fatty-acid profiles under TR were different from those of the T20 condition. Specifically, the contents of octadecanoic acid (C18:0), octadecenoic acid (C18:1), hexadecadienoic acid (C16:2), and octadecadienoic acid (C18:2) increased the most under TR0D. The relative levels of metabolites such as 尾-alanine, glutamine, glycine, isoleucine, proline, valine, and myo-inositol, which act as osmolytes, and bioactive compounds such as neophytadiene and ascorbic acid were increased under TR conditions on day 21. Among the metabolites, the contents of neophytadiene and ascorbic acid were further investigated, and the content of ascorbic acid was highest on day 14 under the TR7D condition, while the content of neophytadiene was highest on day 21 under the TR0D and TR14D condition. Therefore, we suggest that a TR from 20 to 10 掳C could enhance the production in C. vulgaris cultures of bioactive fatty acids such as C18:1, C16:2, and C18:2 (TR0D), organic osmolytes such as 尾-alanine, glutamine, glycine, isoleucine, proline, valine, and myo-inositol (TR conditions), ascorbic acid (TR7D), and neophytadiene (TR0D and TR14D). This study involved multiple reactions and reactants, such as Methyl nonadecanoate (cas: 1731-94-8Application In Synthesis of Methyl nonadecanoate).
Methyl nonadecanoate (cas: 1731-94-8) belongs to esters. Esters are also usually derived from carboxylic acids. It may also be obtained by reaction of acid anhydride or acid halides with alcohols or by the reaction of salts of carboxylic acids with alkyl halides. Esters contain a carbonyl center, which gives rise to 120掳 C鈥揅鈥揙 and O鈥揅鈥揙 angles. Unlike amides, esters are structurally flexible functional groups because rotation about the C鈥揙鈥揅 bonds has a low barrier. Their flexibility and low polarity is manifested in their physical properties; they tend to be less rigid (lower melting point) and more volatile (lower boiling point) than the corresponding amides. Application In Synthesis of Methyl nonadecanoate
Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics