《Aqueous Metabolite Trends for the Progression of Nonalcoholic Fatty Liver Disease in Female Bariatric Surgery Patients by Targeted 1H-NMR Metabolomics》 was published in Metabolites in 2021. These research results belong to Robinson, Emma J.; Taddeo, Matthew C.; Chu, Xin; Shi, Weixing; Wood, Craig; Still, Christopher; Rovnyak, Virginia G.; Rovnyak, David. Quality Control of Methyl 3-oxovalerate The article mentions the following:
Determining biomarkers and better characterizing the biochem. progression of nonalcoholic fatty liver disease (NAFLD) remains a clin. challenge. A targeted 1H-NMR study of serum, combined with clin. variables, detected and localized biomarkers to stages of NAFLD in morbidly obese females. Pre-surgery serum samples from 100 middle-aged, morbidly obese female subjects, grouped on gold-standard liver wedge biopsies (non-NAFLD; steatosis; and fibrosis) were collected, extracted, and analyzed in aqueous (D2O) buffer (1H, 600 MHz). Profiled concentrations were subjected to exploratory statistical anal. Metabolites varying significantly between the non-NAFLD and steatosis groups included the ketone bodies 3-hydroxybutyrate (↓; p = 0.035) and acetone (↓; p = 0.012), and also alanine (←; p = 0.004) and a putative pyruvate signal (←; p = 0.003). In contrast, the steatosis and fibrosis groups were characterized by 2-hydroxyisovalerate (←; p = 0.023), betaine (↓; p = 0.008), hypoxanthine (↓; p = 0.003), taurine (↓; p = 0.001), 2-hydroxybutyrate (←; p = 0.045), 3-hydroxyisobutyrate (←; p = 0.046), and increasing medium chain fatty acids. Exploratory classification models with and without clin. variables exhibited overall success rates ca. 75-85%. In the study conditions, inhibition of fatty acid oxidation and disruption of the hepatic urea cycle are supported as early features of NAFLD that continue in fibrosis. In fibrosis, markers support inflammation, hepatocyte damage, and decreased liver function. Complementarity of NMR concentrations and clin. information in classification models is shown. A broader hypothesis that standard-of-care sera can yield metabolomic information is supported. In the experiment, the researchers used Methyl 3-oxovalerate(cas: 30414-53-0Quality Control of Methyl 3-oxovalerate)
Methyl 3-oxovalerate(cas: 30414-53-0) belongs to ketone compounds. They are most widely used as solvents, especially in industries manufacturing explosives, lacquers, paints, and textiles. Ketones are also used in tanning, as preservatives, and in hydraulic fluids.Quality Control of Methyl 3-oxovalerate
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