Quality Control of Methyl 4-fluoro-3-nitrobenzoateIn 2020 ,《Discovery of triazoloquinoxaline as novel STING agonists via structure-based virtual screening》 was published in Bioorganic Chemistry. The article was written by Hou, Hui; Yang, Ruirui; Liu, Xiaohong; Wu, Xiaolong; Zhang, Sulin; Chen, Kaixian; Zheng, Mingyue. The article contains the following contents:
Stimulator of interferon genes (STING) is an endoplasmic reticulum adaptor facilitating innate immune signaling. Activation of STING leads to expression of interferons (IFNs) and pro-inflammatory cytokines which is associated with antiviral and antitumor responses. It is imperative to discovery potent compounds that precisely modulate STING. Herein, we describe the discovery of triazoloquinoxaline 1a(I) as a novel STING agonist via Structure-based Virtual Screening. Specifically, biochem. and cell-based assays suggested that 1a stimulated concentration-dependently mRNA expression of IFNβ, CXCL-10 and IL-6. Furthermore, 1a significantly induced phosphorylation of STING, TANK-binding kinases 1 (TBK1) and interferon regulatory factor 3 (IRF3), suggesting the activation of STING and its downstream TBK1-IRF3 signaling axis. In addition, 1a activated secretion of secreted alk. phosphatase (SEAP) in dose-dependent manner and EC50 was 16.77 ± 3.814μM, which is comparable with EC50 of 2’3′-cGAMP (9.212 ± 2.229μM). These studies revealed that 1a is a promising STING agonist possessing the potential to be further developed for antiviral and antitumor treatment. In the experimental materials used by the author, we found Methyl 4-fluoro-3-nitrobenzoate(cas: 329-59-9Quality Control of Methyl 4-fluoro-3-nitrobenzoate)
Methyl 4-fluoro-3-nitrobenzoate(cas: 329-59-9) belongs to methyl benzoate. Methyl benzoate reacts at both the ring and the ester, depending on the substrate. Electrophiles attack the ring, illustrated by acid-catalysed nitration with nitric acid to give methyl 3-nitrobenzoate.Quality Control of Methyl 4-fluoro-3-nitrobenzoate
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