Wei, H.-s.; Qin, J.-h.; Cao, Y.-z.; Li, K.-b.; Yin, J. published their research in Insect Molecular Biology in 2021. The article was titled ãTwo classic OBPs modulate the responses of female Holotrichia oblita to three major ester host plant volatilesã?Quality Control of Methyl Salicylate The article contains the following contents:
Insects possess a fairly sophisticated olfactory system in their antennae to detect odorants essential for their survival and reproduction Among them, insect first perceives odor sources by odorant-binding proteins (OBPs) to locate host-plants. Me salicylate, (Z)-3-hexenyl acetate and di-Bu phthalate are major volatile components of Ulmus pumila and Ricinus communis and elicit strong responses of the scarab beetle Holotrichia oblita adults. However, olfactory perception of the scarab beetle to these odorant compounds is unclear. In the current study, we cloned the OBP6 and OBP7 of H. oblita. The expression pattern shows that the two genes were highly expressed in the antennae of female beetles. Binding assays verified that the HoblOBP6 had a better binding affinity to Me salicylate, and so did HoblOBP7 to (Z)-3-hexenyl acetate and di-Bu phthalate. The effect on the responses of female beetles to the three compounds was decreased significantly after these two genes were silenced by RNA interference. These results indicate that HoblOBP6 and HoblOBP7 are essential for female H. oblita perception of Me salicylate, (Z)-3-hexenyl acetate and di-Bu phthalate. Our study provides important insights into the olfactory mechanism of female H. oblita to ester plant volatiles and could facilitate the development of potential pest control strategies in the field. In the experiment, the researchers used many compounds, for example, Methyl Salicylate(cas: 119-36-8Quality Control of Methyl Salicylate)
Methyl Salicylate(cas: 119-36-8) has been used: as a component of clarifying solution for treating Mongolian gerbil cochlea intact for immunofluorescence analysis, as a plant elicitor to test its effect on reducing the whitefly population from tomato plants.Quality Control of Methyl Salicylate
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