Watanabe, Rei; Hu, Yaping; Iio, Keita; Yoneda, Kozo; Hattori, Atsunori; Arai, Atsushi; Kigoshi, Hideo; Kita, Masaki published an article in 2018, the title of the article was Specific protein-labeling and ligand-binding position analysis with amidopyrene probes as LDI MS tags.Product Details of 79642-50-5 And the article contains the following content:
To readily analyze the binding mode of protein-ligand interactions, we developed ligand-bound-type and ligand-dissociation-type probes having 6-amidopyrene (apy) as a detecting group. Matrix- and label-assisted laser desorption/ionization mass spectrometry (MALDI and LA-LDI MS) analyses and a covalent docking simulation using these probes precisely determined the binding position of the ligand biotin on the target protein avidin (RMSD = 0.786 and 0.332 Å). Our apy-probe-labeling method may be useful for determining the unknown ligand-binding sites of various target proteins. The experimental process involved the reaction of Bis(2,5-dioxopyrrolidin-1-yl) glutarate(cas: 79642-50-5).Product Details of 79642-50-5
The Article related to protein labeling ligand interaction amidopyrene probe ldi mass spectrometry, Biochemical Methods: Synthesis and other aspects.Product Details of 79642-50-5
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