Raja, Sebastian; Hamouda, Ahmed E. I.; de Toledo, Marcelo A. S.; Hu, Chaolei; Bernardo, Marcela P.; Schalla, Carmen; Leite, Liliane S. F.; Buhl, Eva Miriam; Dreschers, Stephan; Pich, Andrij; Zenke, Martin; Mattoso, Luiz H. C.; Sechi, Antonio published their research in Biomacromolecules in 2021. The article was titled ãFunctionalized Cellulose Nanocrystals for Cellular Labeling and Bioimagingã?Formula: C11H24N2O2 The article contains the following contents:
Cellulose nanocrystals (CNCs) are unique and promising natural nanomaterials that can be extracted from native cellulose fibers by acid hydrolysis. In this study, we developed chem. modified CNC derivatives by covalent tethering of PEGylated biotin and perylenediimide (PDI)-based near-IR organic dye and evaluated their suitability for labeling and imaging of different cell lines including J774A.1 macrophages, NIH-3T3 fibroblasts, HeLa adenocarcinoma cells, and primary murine dendritic cells. PDI-labeled CNCs showed a superior photostability compared to similar com. available dyes under long periods of constant and high-intensity illumination. All CNC derivatives displayed excellent cytocompatibility toward all cell types and efficiently labeled cells in a dose-dependent manner. Moreover, CNCs were effectively internalized and localized in the cytoplasm around perinuclear areas. Thus, our findings demonstrate the suitability of these new CNC derivatives for labeling, imaging, and long-time tracking of a variety of cell lines and primary cells. The experimental part of the paper was very detailed, including the reaction process of N-Boc-1,6-Diaminohexane(cas: 51857-17-1Formula: C11H24N2O2)
N-Boc-1,6-Diaminohexane(cas: 51857-17-1) can be used as a linear hexyl spacer (C6-spacer) to synthesize biodegradable poly(disulfide amine)s for gene delivery, a multifunctional dendrimer for theranostics and so on.Formula: C11H24N2O2
Referemce:
Ester – Wikipedia,
Ester – an overview | ScienceDirect Topics