Natrajan, Anand; Sharpe, David; Wen, David published an article in 2012, the title of the article was Zwitterionic reagents for labeling, cross-linking and improving the performance of chemiluminescent immunoassays.COA of Formula: C13H14N2O8 And the article contains the following content:
Improving reagent performance in immunoassays both to enhance assay sensitivity and to minimize interference are ongoing challenges in clin. diagnostics. The authors describe herein the syntheses of a new class of hydrophilic reagents containing sulfobetaine zwitterions and their applications. These zwitterionic reagents are potentially useful for improving the properties of immunoassay reagents. The authors demonstrate for the first time that zwitterion labeling is a general and viable strategy for reducing the nonspecific binding of proteins to microparticles and, to improve the aqueous solubility of hydrophobic peptides. The authors also describe the synthesis of zwitterionic crosslinking reagents and demonstrate their utility for peptide conjugation. In automated, chemiluminescent immunoassays, improved assay performance was observed for a hydrophobic, small analyte (theophylline) using an acridinium ester conjugate with a zwitterionic sulfobetaine linker compared to a hexa(ethylene)glycol linker. Sandwich assay performance for a large analyte (TSH) was similar for the two acridinium ester labels. These results indicate that zwitterions are complementary to poly(ethylene)glycol in improving the aqueous solubility and reducing the nonspecific binding of chemiluminescent acridinium ester conjugates. The experimental process involved the reaction of Bis(2,5-dioxopyrrolidin-1-yl) glutarate(cas: 79642-50-5).COA of Formula: C13H14N2O8
The Article related to zwitterionic reagent labeling crosslinking performance chemiluminescent immunoassay, Biochemical Methods: Immunological and other aspects.COA of Formula: C13H14N2O8
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